NEUROD1 antibody
Principal name
NEUROD1 antibody
Alternative names for NEUROD1 antibody
NEUROD1, NEUROD, Neurogenic differentiation factor 1
Ncbi ID
9606, NP_002491, NP_002491.2, NM_002500, NP_035024.1, NM_010894, NM_019218
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C)
Background of NEUROD1 antibody
The NEUROD1 gene encodes a member of the NeuroD family of basic-loop-helix transcription factors. The NEUROD 1 was first described by Lee et al (1995) who observed that the gene was transiently expressed in subsets of neuronal cells of the central and peripheral nervous system at the time of their terminal differentiation into mature neurons. They also showed that ectopic expression of the NEUROD1 gene could convert non-neuronal cells to neuronal cells. The NEUROD1 protein forms heterodimers with other bHLH proteins and activates the transcription of a large set of genes that contain the E-box DNA sequence 5’-CANNTG’-3. NEUROD1 is involved in the regulation of several cell differentiation pathways including the development of retinal ganglion cell, inner ear sensory neurons, and endocrine islet cells. NeuroD1 regulates the expression of numerous genes including the insulin gene. Mutations in the NEUROD1 gene has been associated with type II diabetes mellitus. Defects in expression of NEUROD1 is linked with Maturity onset diabetes of the young type 6 (MODY6). The NEUROD1 protein may be localized in the cytoplasm and the nucleus. In Min 6 insulinoma cells Petersen et al (2002) were able to conclude that MEK-ERK associated phosphorylation of the serine 274 on NEUROD1 induces the translocation of the protein to the nucleus and that this may normally be associated with cell glucose concentration.
General readings
Peng,S.Y., et al., (2005) Biochim. Biophys. Acta 1731 (3), 154-159
Immunoperoxidase of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml]
Immunofluorescence of monoclonal antibody to NEUROD1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged NEUROD1 is approximately 0.03ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
Western Blot analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M02), clone 3D11.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged NEUROD1 is approximately 0.3ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-NEUROD1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; NEUROD1 antibody - N-terminal region (ARP32036_T100) in Human Jurkat cells using Western Blot
Western Blot analysis of NEUROD1 expression in transfected 293T cell line (H00004760-T01) by NEUROD1 MaxPab polyclonal antibody.Lane 1: NEUROD1 transfected lysate(39.16 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NEUROD1 polyclonal antibody ( Cat # PAB3857 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma
Western blot analysis of NEUROD1 polyclonal antibody ( Cat # PAB3858 ) in mouse lung tissue lysate. NEUROD1 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunoprecipitation of NEUROD1 transfected lysate using anti-NEUROD1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NEUROD1 MaxPab mouse polyclonal antibody (B01) (H00004760-B01).
Human HepG2; WB Suggested Anti-NEUROD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; NEUROD1 antibody - middle region (ARP38162_P050) in Human HepG2 cells using Western Blot
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody clone 3D11.
Detection limit for recombinant GST tagged NEUROD1 is approximately 0.3ng/ml as a capture antibody.
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to NEUROD1 on HeLa cell. [antibody concentration 10 µg/ml]
Western Blot analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody clone 3H8.
Detection limit for recombinant GST tagged NEUROD1 is approximately 0.03ng/ml as a capture antibody.
Figure 1. Shows Neuro2A (Mouse neuroblastoma) and SKNSH, 1691 and IMR32 (Human neuroblastoma) cells stained with anti-NEUROD1 Antibody Cat.-No AM32710PU-N (Clone BC18-1.11EG) by the Indirect Immunofluorescence method. The secondary antibody was FITC conjugated anti-Mouse Ig. Nuclear and cytoplasmic staining for NEUROD is observed in all cells. Since the cell lines tested loosely attach to surfaces of chamber slides, the cells were immobilized on adhesion slides for the staining proceedure. The cells were allowed to attach to adhesion slides, fixed in 1% paraformaldehyde, permeabilized in 0.25% Triton X 100 in PBS, blocked with 1% BSA and stained overnight with 5 µg/ml antibody diluted with 1% BSA in PBS. The secondary antibody was diluted with 1% BSA in PBS according to manufacturers instruction and incubated for 2 hours.
Figure 2. Shows K562 (chronic myelogenous leukemia), RDES (Ewings sarcoma), SKES (Ewings) sarcoma and PFSK1 (Primitive neuroectodermal tumor) cells stained with anti-NEUROD1 Antibody Cat.-No AM32710PU-N (Clone BC18-1.11EG) by the indirect immunoperoxidase method.
K562 was used as a negative control and showed no staining whereas RDES, SKES-1 and PFSK-1 cells showed weak nuclear but strong cytoplasmic staining. Since the cell lines tested loosely attach to surfaces of chamber slides, the cells were immobilized on adhesion slides for the staining proceedure. The cells were fixed in 1% paraformaldehyde, permeabilized in 0.25% Triton X 100 in PBS, blocked with 1% BSA and stained overnight with 5 µg/ml antibody diluted with 1% BSA in PBS. The peroxidase conjugated anti Mouse Ig secondary antibody was used at 1/1000 in 1% BSA in PBS. DAB substrate was used with nickle chloride enhancement to give dark purple/black staining for positive reaction. The substrate was added for 10 minutes, extensively washed, counterstained in diluted hematoxylene, dehydrated, cleared in xylene and mounted.
K562 was used as a negative control and showed no staining whereas RDES, SKES-1 and PFSK-1 cells showed weak nuclear but strong cytoplasmic staining. Since the cell lines tested loosely attach to surfaces of chamber slides, the cells were immobilized on adhesion slides for the staining proceedure. The cells were fixed in 1% paraformaldehyde, permeabilized in 0.25% Triton X 100 in PBS, blocked with 1% BSA and stained overnight with 5 µg/ml antibody diluted with 1% BSA in PBS. The peroxidase conjugated anti Mouse Ig secondary antibody was used at 1/1000 in 1% BSA in PBS. DAB substrate was used with nickle chloride enhancement to give dark purple/black staining for positive reaction. The substrate was added for 10 minutes, extensively washed, counterstained in diluted hematoxylene, dehydrated, cleared in xylene and mounted.
Figure 3. Shows reactivity of anti-NEUROD1 Antibody Cat.-No AM32710PU-N (Clone BC18-1.11EG) against SHSY5Y, Kelly and SKNSH neuroblastoma cell lysates by Western blotting. For each cell line, a band of approximately 45kD was observed. This molecular size corresponds to the expected size for NEUROD1. The antibody was used at 2 µg/ml and incubated overnight at 4°C The peroxidase conjugated anti-Mouse Ig secondary antibody was used at 1/10000 dilution and incubated for 2 hours. The blot was developed using enhanced chemiluminescence and image captured on radiographic films.
Western blot analysis of extracts from HeLa cells treated with UV using Neuro D (Phospho-Ser274) Antibody AP55826PU-N.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HeLa cells, using Neuro D (Ab-274) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of hNeuroD1-Q30 (Cat. #TA324696) in HepG2 cell line lysates (35ug/lane). NEUROD1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
ES cells were transiently transfected with flag-tagged mouse NeuroD1 (tagged on N-term).Fixed 24h post transfection.Stained for flag tag (red) to check that some cells express protein. Most protein was in nucleus but some was cytoplasmic. Stained with NeuroD1 N-term antibodies at 1:100. NeuroD1 N-term antibody showed strong and clear staining with similar pattern to the flag staining.(Supplied by Sally Lowell,Edinburgh University)
IF image of HepG2 cell stained with hNeuroD1-Q30(Cat#TA324696). HepG2 cells were incubated with hNeuroD1-Q30 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .hNeuroD1-Q30 immunoreactivity is localized to vesicles significantly.
NeuroD1 Antibody (I333) (Cat. #TA324697) western blot analysis in Y79 cell line ,mouse cerebellum and rat brain tissue lysates (35ug/lane).This demonstrates the NeuroD1 antibody detected the NeuroD1 protein (arrow).
WB Suggested Anti-NEUROD1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate
WB Suggested Anti-NEUROD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate
Western blot analysis on HeLa cell lysate using Neuro D Antibody
Western blot analysis of extracts from HeLa cells, treated with UV (15mins), using Neuro D (Phospho-Ser274) antibody.The lane on the right is treated with the synthesized peptide.
- Quick-Filters:
- Highly Validated (1)
- Overnight Delivery (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324696 | NEUROD1 (N-term) antibody |
||||||
| Rabbit polyclonal NeuroD1 Antibody (N-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | P |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
||||||
| TA312013 | NEUROD1 antibody |
||||||
| Rabbit polyclonal Neuro D (Ab-274) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA312014 | NEUROD1 antibody |
||||||
| Rabbit polyclonal Neuro D (Ser274) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324697 | NEUROD1 (C-term) antibody |
||||||
| Rabbit polyclonal NeuroD1 Antibody (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347337 | NEUROD1 antibody |
||||||
| Rabbit Polyclonal Anti-Neuro D Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM31117PU-N | NEUROD1 (201-300) antibody |
||||||
 |
Mouse | IgG | 3D11 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AM32710PU-N | NEUROD1 antibody |
||||||
 |
Mouse | IgG1 | BC18-1.11EG | Purified | Hu, Ms | C, ICC/IF, WB |
0.1 mg /
€260.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP55826PU-N | NEUROD1 pSer274 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| AP55826PU-S | NEUROD1 pSer274 antibody |
||||||
|
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
| GT15231-100 | NEUROD1 antibody |
||||||
 |
Goat | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 mg /
€450.00
|
|
| Neuromics Antibodies | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301987 | NEUROD1 |
||||
| Recombinant protein of human neurogenic differentiation 1 (NEUROD1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400891 | NEUROD1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC201987L2V | Lenti ORF particles, NEUROD1 (mGFP-tagged) - Human neurogenic differentiation 1 (NEUROD1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205460L2V | Lenti ORF particles, Neurod1 (GFP-tagged) - Mouse neurogenic differentiation 1 (Neurod1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205460L1V | Lenti ORF particles, Neurod1 (Myc-DDK-tagged) - Mouse neurogenic differentiation 1 (Neurod1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR210907L2V | Lenti ORF particles, Neurod1 (GFP-tagged ORF) - Rat neurogenic differentiation 1 (Neurod1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR210907L1V | Lenti ORF particles, Neurod1 (Myc-DDK-tagged ORF) - Rat neurogenic differentiation 1 (Neurod1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||