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NFAT5 antibody

Principal name

NFAT5 antibody

Alternative names for NFAT5 antibody

NFAT5, KIAA0827, TONEBP, Nuclear factor of activated T-cells 5

SwissProt ID

D3ZGB1, O94916 (Human), Q9WV30

Gene ID

10725 (NFAT5), 54446 (Nfat5)

Ncbi ID

9606, AAF79117, NP_006590, NP_006590.1, NM_001113178, 10090, NM_133957, NM_006599, NM_173214, NM_138714, NM_138713

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of NFAT5 antibody

NFAT5 is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with D elements.The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with D elements. Multiple transcript variants encoding different isoforms have been found for this gene.

General readings

Pan,S., et al., (2000) Biochem. Biophys. Res. Commun. 272 (3), 765-776

HT-29; . Lanes: Lane 1: 20ug HT 29 lysate blocked with 5% FBS Lane 2: 20ug HT 29 lysate blocked with no FBS Lane 3: 20ug HT-29 lyaste blocked with no PBS + 100 mM NaCl . Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-rabbit-HRP. Secondary Antibody Dilution: 1:1000. Gene Name: NFAT5. Submitted by: Manuel Fresno, CBMSO.; NFAT5 antibody - middle region (ARP37465_P050) in HT-29 cells using Western Blot

ARP37465_P050

Mouse SP2/0; WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: SP2/0 cell lysate; NFAT5 antibody - middle region (ARP37465_P050) in Mouse SP2/0 cells using Western Blot

ARP37465_P050

Western Blot analysis of NFAT5 expression in transfected 293T cell line (H00010725-T01) by NFAT5 MaxPab polyclonal antibody.Lane 1: NFAT5 transfected lysate(163.24 KDa).Lane 2: Non-transfected lysate.

H00010725-B01

Human MCF-7; WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; NFAT5 antibody - middle region (ARP38831_P050) in Human MCF-7 cells using Western Blot

ARP38831_P050

Immunofluorescence analysis of COS7 cells, using NFAT5 (Phospho-Ser1197) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells treated with forskolin 40nM 30', using NFAT5/TonEBP (Phospho-Ser155) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using NFAT5 (Ab-1197) Antibody. The picture on the right is treated with the synthesized peptide.

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver and ovarian cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using NFAT5 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200)

TA322478

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver and ovarian cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using NFAT5 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)

TA322478

Lanes:Lane 1: 20ug HT 29 lysate blocked with 5% FBS Lane 2: 20ug HT 29 lysate blocked with no FBS Lane 3: 20ug HT-29 lyaste blocked with no PBS + 100 mM NaCl; Primary Antibody Dilution:1:1000; Secondary Antibody:Goat anti-rabbit-HRP; Secondary Antibody Di

TA329984

WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: SP2/0 cell lysate

TA329984

WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysate

TA343626

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA322478	NFAT5 antibody
	Anti-NFAT5 Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image

Lysates

Catalog No.
LY426386	NFAT5 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR223442L1V	Lenti ORF particles, Nfat5 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant a, 200 uL, >10^7 TU/mL
		200 µl / €2,280.00
		OriGene Technologies, Inc.

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NFAT5 antibody

Principal name

Alternative names for NFAT5 antibody

SwissProt ID

Gene ID

Ncbi ID

Available reactivities

Available hosts

Available applications

Background of NFAT5 antibody

General readings

Primary Antibodies

NFAT5 antibody

Lysates

NFAT5 overexpression lysate

Mouse Lenti ORF Particles

Lenti ORF particles, Nfat5 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant a, 200 uL, >10^7 TU/mL

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