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NFATC2 antibody

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Principal name

NFATC2 antibody

Alternative names for NFATC2 antibody

NFATC2, NFAT-1, NFATP, Nuclear factor of activated T-cells, cytoplasmic 2,T-cell transcription factor NFAT1, NFAT pre-existing subunit

SwissProt ID

Q13469 (Human), Q60591 (Mouse)

Gene ID

4773 (NFATC2), 18019 (Nfatc2)

Ncbi ID

9606, NP_035029, NP_036472.2, NM_173091, NM_012340, NP_775114, NP_036472, NP_001129493, NP_001032254.1, BC064006, NM_001037177, NM_001037178, NM_010899, NM_001136073, NM_001136021

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Dot blot (Dot), Paraffin Sections (P)

Background of NFATC2 antibody

NFATC2(Nuclear factor of activated T-cells, cytoplasmic 2),also known as NFATP or the 'preexisting component' of NFAT, is present in the cytosolic fraction of unstimulated T cells, which is also a member of the nuclear factor of activated T cells (NFAT) family. The NFATC2 gene is mapped on 20q13.2. NFATC2 is highly homologous to NFATC1 over a limited domain which shows similarity to the Dorsal/Rel family but has a wider tissue distribution. Ectopic expression of NFATC2 inhibited the basal activity of the human CDK4 promoter. Additionally, both Calna-/- and Nfatc2 -/- mice had elevated protein levels of Cdk4, confirming a negative regulatory role for the calcineurin/NFAT pathway. NFATC2 controls myoblast fusion at a specific stage of myogenesis after the initial formation of a myotube and is necessary for further cell growth. Overexpression of NFATC2 promoted differentiation of osteoclast precursor cells into tartrate-resistant acid phosphatase-positive (TRAP-positive) multinucleated osteoclast-like cells even in the absence of RANKL

General readings

Celil Aydemir AB, Lee S, Won Kim D, Gardner TR, Prince D, Mok Ahn J, Young-In Lee F, Nuclear factor of activated T cell mediates proinflammatory gene expression in response
to mechanotransduction. Ann. N. Y. Acad. Sci. 2007 Nov 1117 : 138-42.

H00004773-M01 - NFATC2
Detection limit for recombinant GST tagged NFATC2 is approximately 0.1ng/ml as a capture antibody.
ARP37106_T100 - NFATC2
Mouse SP2/0; WB Suggested Anti-NFATC2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: SP2/0 cell lysate; NFATC2 antibody - N-terminal region (ARP37106_T100) in Mouse SP2/0 cells using Western Blot
AP12851PU-N - NFATC2
Dot blot analysis of anti-NFATC2-pSer330 Phospho-specific Pab (Cat.#AP12851PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
PAB8105 - NFATC2
Dot blot analysis of NFATC2 (phospho S330) polyclonal antibody ( Cat # PAB8105 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
ARP38988_P050 - NFATC2
Human Thymus; Immunohistochemistry with Thymus tissue at an antibody concentration of 5ug/ml using anti-NFATC2 antibody (ARP38988_P050); NFATC2 antibody - C-terminal region (ARP38988_P050) in Human Thymus cells using Immunohistochemistry
ARP38988_P050 - NFATC2
Human HeLa; WB Suggested Anti-NFATC2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; NFATC2 antibody - C-terminal region (ARP38988_P050) in Human HeLa cells using Western Blot
TP313966 - NFATC2
TP315180 - NFATC2
TP326803 - NFATC2
TA305927 - NFATC2
TA305927 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA322011 - NFATC2
Predicted cell location: Cytoplasm or Nucleus. Positive control: Human thyroid and ovarian cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using NFATC2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA322011 - NFATC2
Predicted cell location: Cytoplasm or Nucleus. Positive control: Human thyroid and ovarian cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using NFATC2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA323876 - NFATC2
Predicted band size: 98 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/650 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)
TA327519 - NFATC2
Western blot analysis of extracts of mouse fetal brain cell line, using NFATC2 antibody.
TA342283 - NFATC2
WB Suggested Anti-NFATC2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: SP2/0 cell lysate
TA343699 - NFATC2
WB Suggested Anti-NFATC2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate
TA343699 - NFATC2
Immunohistochemistry with Thymus tissue at an antibody concentration of 5 ug/ml using anti-NFATC2 antibody
TA352283 - NFATC2
Total cell lysates from Raji cells (lane 1) and mouse splenocytes (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NFATC2 (clone 16A12A31) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.

3 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFATC2 antibody
  Goat Anti-NFATC2 / NFAT1 Antibody  
TA305927 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

NFATC2 antibody
  Anti-NFATC2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm or Nucleus. Positive control: Human thyroid and ovarian cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using NFATC2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

NFATC2 antibody
  Anti-NFATC2 Rabbit Polyclonal Antibody  
Predicted band size: 98 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/650 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

3 Item(s)

per page

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Product Type
  1. Primary Antibody (3)
Clonality
  1. Polyclonal (3)
Target
  1. NFATC2 (3)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (3)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (2)
  2. Goat (1)
Images
  1. 1 image (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. IHC (1)
Manufacturer
  1. OriGene Technologies (3)
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