NFKBIL1 antibody
Principal name
NFKBIL1 antibody
Alternative names for NFKBIL1 antibody
IKBL, IkappaBL, NF-kappa-B inhibitor-like protein 1, Inhibitor of kappa B-like protein, I-kappa-B-like protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1
Ncbi ID
NM_005007, NP_001138433, ABZ92040.1, NM_001144963, NP_004998, NP_001138433.1, NP_035039.1, NM_010909, NM_212509, NM_001144961
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of NFKBIL1 antibody
- Kit Component:
- - KN310950G1, Nfkbil1 gRNA vector 1 in pCas-Guide vector
- KN310950G2, Nfkbil1 gRNA vector 2 in pCas-Guide vector
- KN310950D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Clancy, R.M. et al., Arthritis Rheum. 62(11): 3415-3424 (2010)
Bailey, S.D. et al., Diabetes Care 33(10): 2250-2253 (2010)
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10): 770-777 (2010)
Rose, J.E. et al., Mol. Med. 16 (7-8): 247-253 (2010)
Owecki, M.K. et al., Pol. Merkur. Lekarski 28(167): 366-370 (2010)
Human Stomach; WB Suggested Anti-NFKBIL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Stomach; NFKBIL1 antibody - N-terminal region (ARP38540_P050) in Human Stomach cells using Western Blot
Confocal immunofluorescent analysis of MDA-MB435 cells using NFKBIL1 antibody Cat.-No. AP52865PU-N, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in CEM cell line lysates (35ug/lane) using NFKBIL1 antibody Cat.-No. AP52865PU-N. This demonstrates this antibody detected the NFKBIL1 protein (arrow).
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell
(left histogram) using NFKBIL1 antibody Cat.-No. AP52865PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
(left histogram) using NFKBIL1 antibody Cat.-No. AP52865PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
Immunohistochemistry analysis in human brain tissue (Formalin-fixed, Paraffin-embedded) using NFKBIL1 antibody Cat.-No. AP52866PU-N (C-term), followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of MDA-MB435 cells using NFKBIL1 antibody Cat.-No. AP52866PU-N (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane) using NFKBIL1 antibody Cat.-No. AP52866PU-N (C-term). This demonstrates this antibody detected the NFKBIL1 protein (arrow).
WB Suggested Anti-NFKBIL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Stomach
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52865PU-N | NFKBIL1 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
|||||||
| AP52866PU-N | NFKBIL1 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311251 | NFKBIL1 (transcript variant 1) |
||||
| Recombinant protein of human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 (NFKBIL1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY428610 | NFKBIL1 overexpression lysate |
|
 |
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205949L2V | Lenti ORF particles, Nfkbil1 (GFP-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 (Nfkbil1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205949L1V | Lenti ORF particles, Nfkbil1 (Myc-DDK-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 (Nfkbil1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209388L2V | Lenti ORF particles, Nfkbil1 (GFP-tagged ORF) - Rat nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 (Nfkbil1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR209388L1V | Lenti ORF particles, Nfkbil1 (Myc-DDK-tagged ORF) - Rat nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 (Nfkbil1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||