NFS antibody
Principal name
NFS antibody
Alternative names for NFS antibody
Cysteine desulfurase, NIFS, HUSSY-08
Gene ID
9054 (NFS1)
Ncbi ID
NP_001185918, NP_066923, 9606, NP_066923.3, NP_001185918.1, NM_021100
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of NFS antibody
- Kit Component:
- - KN209405G1, NFS1 gRNA vector 1 in pCas-Guide vector
- KN209405G2, NFS1 gRNA vector 2 in pCas-Guide vector
- KN209405D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Naamati, A. et al., J. Biol. Chem. 284(44): 30200-30208 (2009)
Marelja, Z. et al., J. Biol. Chem. 283(37): 25178-25185 (2008)
Wu, C. et al., Proteomics 7(11): 1775-1785 (2007)
Lamesch, P. et al., Genomics 89(3): 307-315 (2007)
Biederbick, A. et al., Mol. Cell. Biol. 26(15): 5675-5687 (2006)
Western blot analysis in K562 cell line lysates (35ug/lane) using NFS antibody Cat.-No. AP52867PU-N. This demonstrates this antibody detected the NFS1 protein (arrow).
Human Jurkat; WB Suggested Anti-NFS1 Antibody Titration: 0.3125ug/ml. Positive Control: Jurkat cell lysate. NFS1 is supported by BioGPS gene expression data to be expressed in Jurkat.; NFS1 antibody - middle region (ARP33115_T100) in Human Jurkat cells using Western Blot
Western Blot analysis of NFS1 expression in transfected 293T cell line (H00009054-T01) by NFS1 MaxPab polyclonal antibody.Lane1:NFS1 transfected lysate(50.27 KDa).Lane2:Non-transfected lysate.
Western Blot analysis of NFS1 expression in transfected 293T cell line (H00009054-T01) by NFS1 MaxPab polyclonal antibody.Lane1:NFS1 transfected lysate(50.27 KDa).Lane2:Non-transfected lysate.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805491)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805491)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805491)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805491)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFS1 (RC209405, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFS1.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805820)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805820)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805820)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFS1 (RC209405, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFS1.
WB Suggested Anti-NFS1 Antibody Titration: 0.3125ug/ml; Positive Control: Jurkat cell lysateNFS1 is supported by BioGPS gene expression data to be expressed in Jurkat
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA805491 | NFS antibody |
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| NFS1 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5) | |||||||
 |
Mouse | IgG2b | OTI2H5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA805820 | NFS antibody |
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| NFS1 mouse monoclonal antibody, clone OTI5D1 (formerly 5D1) | |||||||
 |
Mouse | IgG2a | OTI5D1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| AP52867PU-N | NFS (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
