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NFYA antibody

Principal name

NFYA antibody

Alternative names for NFYA antibody

Nuclear transcription factor Y subunit alpha

SwissProt ID

P23511 (Human)

Gene ID

4800 (NFYA)

Ncbi ID

NP_002496.1, 9606, NP_002496, NM_002505

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NFYA antibody

NFY is a transcription factor composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. NFY binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit.
NFY recognizes a CCAAT motif upstream of gene promoters and is probably involved in the regulation of a variety of genes.
Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NFY in DNA binding assays. The conserved core sequences of NFYB and NFYC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.

General readings

Zhu,Q.S., et al., (2004) J.Biol.Chem.279(29),29902-29910

Human Liver; NFYA antibody - C-terminal region (ARP31263_T100) in Human Liver cells using Immunohistochemistry

ARP31263_T100

Human Jurkat; WB Suggested Anti-NFYA Antibody Titration: 5.0-8.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. NFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; NFYA antibody - C-terminal region (ARP31263_T100) in Human Jurkat cells using Western Blot

ARP31263_T100

Human Liver; NFYA antibody - C-terminal region (ARP31380_P050) in Human Liver cells using Immunohistochemistry

ARP31380_P050

Human Muscle; HumanMuscle; NFYA antibody - C-terminal region (ARP31380_P050) in Human Muscle cells using Immunohistochemistry

ARP31380_P050

Human Jurkat; WB Suggested Anti-NFYA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. NFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; NFYA antibody - C-terminal region (ARP31380_P050) in Human Jurkat cells using Western Blot

ARP31380_P050

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE)

AP07266SU-N

CHO-7 cells were cultured in the absence ( I ) or presence ( S ) of cholesterol.Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein were subjected to analysis by SDS-PAGE and immunoblot.Using NFYA polyclonal antibody ( Cat # PAB11297 ) at a 1 : 1000 dilution.

PAB11297

Western blot analysis of extracts from HepG2/293/COLO205 cells, using NFYA Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of NFYA staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of NFYA staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of NFYA expression in A431 (A); K562 (B); NIH3T3 (C) whole cell lysates.

TP760255

Western blot analysis of extracts from 293 cells, HepG2 cells and COLO205 cells, using NFYA antibody.The lane on the right is treated with the synthesized peptide.

TA314258

WB of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

TA319426

WB of Rabbit Anti-NF-Y (A Subunit) Antibody. Lane 1: CHO-7 cells were cultured in the absence (I) of cholesterol. Lane 2: CHO-7 cells were cultured in the presence (S) of cholesterol. Load: 35 µg per lane. Primary antibody: NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

TA319427

Immunohistochemistry of Rabbit anti-NF-Y (A Subunit) antibody. Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: NF-Y antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: NF-Y is nuclear. Staining: NF-Y subunit A as precipitated red signal with hematoxylin purple nuclear counterstain.

TA319427

WB Suggested Anti-NFYA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

TA329254

Human Liver

TA329254

Human Muscle

TA329254

WB Suggested Anti-NFYA Antibody Titration: 5.0-8.0ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

TA329233

Human Liver

TA329233

Lysates

Catalog No.
LY400893	NFYA overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

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