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NFYB antibody

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Principal name

NFYB antibody

Alternative names for NFYB antibody

HAP3, Nuclear transcription factor Y subunit beta

SwissProt ID

P25208 (Human), P63139 (Mouse), P63140 (Rat)

Gene ID

4801 (NFYB), 18045 (Nfyb), 25336

Ncbi ID

NP_006157.1, 9606, NP_006157, NP_035044.1, NM_010914, NM_031553, NM_006166

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of NFYB antibody

NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

General readings

Kimura,K., (2006) Genome Res. 16 (1), 55-65

H00004801-M01 - NFYB
Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
H00004801-M01 - NFYB
Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi ( Cat # H00004801-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 (Cat # H00004801-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00004801-M01 - NFYB
Detection limit for recombinant GST tagged NFYB is approximately 0.1ng/ml as a capture antibody.
H00004801-M02 - NFYB
Detection limit for recombinant GST tagged NFYB is approximately 0.1ng/ml as a capture antibody.
H00004801-M03 - NFYB
Detection limit for recombinant GST tagged NFYB is approximately 0.03ng/ml as a capture antibody.
H00004801-M06 - NFYB
Detection limit for recombinant GST tagged NFYB is approximately 0.3ng/ml as a capture antibody.
ARP38748_P050 - NFYB
Transfected 293T; WB Suggested Anti-NFYB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; NFYB antibody - N-terminal region (ARP38748_P050) in Transfected 293T cells using Western Blot
AP07863SU-N - NFYB
Figure 1. Staining NFYB in Thymus by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
ARP31262_P050 - NFYB
Transfected 293T; WB Suggested Anti-NFYB Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; NFYB antibody - middle region (ARP31262_P050) in Transfected 293T cells using Western Blot
C10477-1 - NFYB
Western blot analysis of extracts from 293 cells, using NFYB Antibody. The lane on the right is treated with the synthesized peptide.
TP302749 - NFYB
TA319442 - NFYB
Immunohistochemistry. Anti-NF-Y (B Subunit) antibody was diluted 1:500 to detect NF-YB in human thymus tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
TA324547 - NFYB
NFYB Antibody (N-term) (Cat. #TA324547) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow).
TA324547 - NFYB
IF image of Hela cell stained with NFYB Antibody (N-term)(Cat#TA324547). Hela cells were incubated with NFYB primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . NFYB immunoreactivity is localized to nucleus significantly.
TA343587 - NFYB
WB Suggested Anti-NFYB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFYB antibody
  Rabbit polyclonal anti-NF-Y antibody  
Immunohistochemistry. Anti-NF-Y (B Subunit) antibody was diluted 1:500 to detect NF-YB in human thymus tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Rabbit Serum Hu, Ms E, EMSA, IP, WB
0.1 ml / €515.00
  OriGene Technologies, Inc.

NFYB antibody
  Rabbit polyclonal NFYB Antibody (N-term)  
NFYB Antibody (N-term) (Cat. #TA324547) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow). Rabbit IgG Purified Bov, Chk, Hu, Ms, Rt ICC/IF, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

NFYB (N-term) antibody
Figure 1. Staining NFYB in Thymus by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Hu E, IP, P, WB
50 µl / €425.00
  OriGene Technologies GmbH

NFYB antibody
NFYB Rabbit Ig Fraction Hu, Ms E, EMSA, IP, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NFYB
  Recombinant protein of human nuclear transcription factor Y, beta (NFYB)  
NFYB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nfyb (GFP-tagged) - Mouse nuclear transcription factor-Y beta (Nfyb), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nfyb (GFP-tagged) - Mouse nuclear transcription factor-Y beta (Nfyb), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Nfyb (Myc-DDK-tagged) - Mouse nuclear transcription factor-Y beta (Nfyb), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nfyb (Myc-DDK-tagged) - Mouse nuclear transcription factor-Y beta (Nfyb), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nfyb (GFP-tagged ORF) - Rat nuclear transcription factor-Y beta (Nfyb), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nfyb (GFP-tagged ORF) - Rat nuclear transcription factor-Y beta (Nfyb), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Nfyb (Myc-DDK-tagged ORF) - Rat nuclear transcription factor-Y beta (Nfyb), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nfyb (Myc-DDK-tagged ORF) - Rat nuclear transcription factor-Y beta (Nfyb), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

9 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (4)
  3. Protein | Growth Factor (1)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
Target
  1. NFYB (5)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (3)
  3. Bovine (Bov) (1)
  4. Chicken (Chk) (1)
  5. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (3)
  3. Immunoprecipitation (IP) (3)
  4. Electrophoretic Mobility Shift Assay (EMSA) (2)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  6. Paraffin Sections (P) (1)
Presentation
  1. Ig Fraction (1)
  2. Purified (1)
  3. Serum (1)
Host
  1. Rabbit (4)
Images
  1. 1 image (4)
Images: Applications
  1. Immunofluorescence (IF) (1)
  2. IHC (1)
  3. Paraffin Sections (P) (1)
  4. Western blot / Immunoblot (WB) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (9)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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