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NFYC antibody

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Principal name

NFYC antibody

Alternative names for NFYC antibody

Nuclear transcription factor Y subunit gamma, Transactivator HSM-1/2

SwissProt ID

Q13952 (Human)

Gene ID

4802 (NFYC)

Ncbi ID

9606, CAI16533, NP_055038, NP_001136059.1, NP_055038.2, AAH05003.1, NM_001142587, NM_001142588, NM_001142589, NM_001142590, NP_001136061, NM_014223

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of NFYC antibody

NFYC is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. NFYC forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.

General readings

Ichikawa, S. et al., J. Bone Miner. Res. 25(8): 1821-1829 (2010)
Murai-Takeda, A. et al., J. Biol. Chem. 285(11): 8084-8093 (2010)
Ceribelli, M. et al., J. Biol. Chem. 284(49): 34189-34200 (2009)
Yamanaka, K. et al., Genomics 94(4): 219-227 (2009)
Horikawa, Y. et al., Clin. Cancer Res. 14(23): 7956-7962 (2008)

H00004802-M01 - NFYC
Immunoperoxidase of monoclonal antibody to NFYC on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
H00004802-M01 - NFYC
Western blot analysis of NFYC over-expressed 293 cell line, cotransfected with NFYC Validated Chimera RNAi ( Cat # H00004802-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYC monoclonal antibody (M01), clone 1D3 (Cat # H00004802-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00004802-M01 - NFYC
Western Blot analysis of NFYC expression in transfected 293T cell line by NFYC monoclonal antibody (M01), clone 1D3.Lane 1: NFYC transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.
P100681_P050 - NFYC
Human Lung; . Rabbit Anti-NFYC antibody. . Catalog Number:. P100681_P050 . Paraffin Embedded Tissue: Human Lung cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; NFYC antibody (P100681_P050) in Human Lung cells using Immunohistochemistry
P100681_P050 - NFYC
Human Jurkat; WB Suggested Anti-NFYC Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. NFYC is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; NFYC antibody - C-terminal region (P100681_P050) in Human Jurkat cells using Western Blot
H00004802-D01P - NFYC
Western Blot analysis of NFYC expression in transfected 293T cell line (H00004802-T01) by NFYC MaxPab polyclonal antibody.Lane 1: NFYC transfected lysate(37.20 KDa).Lane 2: Non-transfected lysate.
ARP39066_P050 - NFYC
Human Liver; NFYC antibody - C-terminal region (ARP39066_P050) in Human Liver cells using Immunohistochemistry
ARP39066_P050 - NFYC
Human 293T; WB Suggested Anti-NFYC Antibody Titration: 0.25 ug/ml. Positive Control: 293T cells lysate. There is BioGPS gene expression data showing that NFYC is expressed in HEK293T.; NFYC antibody - C-terminal region (ARP39066_P050) in Human 293T cells using Western Blot
AP20495PU-N - NFYC
Immunohistochemistry (IHC) analyzes of NF-YC antibody (Cat.-No.: AP20495PU-N) in paraffin-embedded human liver carcinoma tissue.
AP20495PU-N - NFYC
Western blot (WB) analysis of NF-YC antibody (Cat.-No.: AP20495PU-N) in extracts from HeLa.
C17136-1 - NFYC
Western blot analysis of extracts from HeLa/293 cells, using NFYC Antibody. The lane on the right is treated with the synthesized peptide.
AP52868PU-N - NFYC
Western blot analysis in HepG2 cell line lysates (35ug/lane) using NFYC antibody Cat.-No. AP52868PU-N (N-term). This demonstrates this antibody detected the NFYC protein (arrow).
CPA1811-100ul - NFYC
Immunohistochemical analysis of NFYC staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1811-100ul - NFYC
Western blot analysis of NFYC expression in K562 (A); NIH3T3 (B); HeLa (C); Jurkat (D) whole cell lysates.
TP300060 - NFYC
TP326680 - NFYC
TA314260 - NFYC
Western blot analysis of extracts from 293 cells and HeLa cells, using NFYC antibody.The lane on the right is treated with the synthesized peptide.
TA330041 - NFYC
WB Suggested Anti-NFYC Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateNFYC is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
TA330041 - NFYC
Rabbit Anti-NFYC antibody; Paraffin Embedded Tissue: Human Lung cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
TA343722 - NFYC
Host: Rabbit; Target Name: NFYC; Sample Tissue: Liver Tumor lysates; Antibody Dilution: 1.0 ug/ml
TA343723 - NFYC
WB Suggested Anti-NFYC Antibody Titration: 0.25 ug/ml; Positive Control: 293T cells lysate; There is BioGPS gene expression data showing that NFYC is expressed in HEK293T
TA343723 - NFYC
Human Liver

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFYC antibody
  Rabbit polyclonal anti-NFYC antibody  
Western blot analysis of extracts from 293 cells and HeLa cells, using NFYC antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

NFYC antibody
Immunohistochemistry (IHC) analyzes of NF-YC antibody (Cat.-No.: AP20495PU-N) in paraffin-embedded human liver carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

NFYC (N-term) antibody
Western blot analysis in HepG2 cell line lysates (35ug/lane) using NFYC antibody Cat.-No. AP52868PU-N (N-term). This demonstrates this antibody detected the NFYC protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NFYC (transcript variant 2)
  Recombinant protein of human nuclear transcription factor Y, gamma (NFYC), transcript variant 2  
NFYC Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

NFYC (transcript variant 4)
  Purified recombinant protein of Homo sapiens nuclear transcription factor Y, gamma (NFYC), transcript variant 4  
NFYC Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

NFYC overexpression lysate
NFYC overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

NFYC overexpression lysate
NFYC overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

NFYC overexpression lysate
NFYC overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

NFYC overexpression lysate
NFYC overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

9 Item(s)

per page

Available Filters

Product Type
  1. Lysate (4)
  2. Primary Antibody (3)
  3. Protein | Growth Factor (2)
Clonality
  1. Polyclonal (3)
Target
  1. NFYC (5)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (1)
Host
  1. Rabbit (3)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (2)
Source
  1. HEK293 cells (2)
Manufacturer
  1. OriGene Technologies (9)
Service
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