Nibrin antibody
Principal name
Nibrin antibody
Alternative names for Nibrin antibody
NBN, NBS, NBS1, P95, Nijmegen breakage syndrome protein 1, Cell cycle regulatory protein p95
Ncbi ID
NP_002476, 9606, NP_002476.2, NP_038780, BAG35323.1, NP_038780.3, NM_013752, NM_138873, NM_002485
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C)
Background of Nibrin antibody
NBS1 (Nijmegen breakage syndrome protein 1) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays important role in detection and sigling of D double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint sigling, and as repair-effector in two competing DSB repair pathways: homologous recombition (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintence. NBS1's FHA domain binds phospho-Thr residues in Ser-X-Thr motifs present in D damage proteins, including Mdc1 and Ctp1, whereas, BRCT domains of NBS1 bind Ser-X-Thr motifs when Ser residue is phosphorylated. These phospho-dependent interactions are important for recruiting repair and checkpoint proteins to DSB sites and NBS1 is responsible for MRN complex's nuclear translocation. NBS1 itself does not possess enzymatic activity and contributes to DSB repair primarily by mediating protein-protein interactions at D breakage sites. The central region of NBS1 1 possesses several SQ motifs that are phosphorylated by ATM kise via D damage response. NBS1's C-terminus contains a domain that interacts with ATM and recruits it to DSBs, and induces apoptosis in response to damage. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombition etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis.
General readings
1. Lee JH et al. Direct Activation of ATM by Resveratrol under Oxidizing Conditions. PLoS One9:e97969 (2014). WB ; Human .PubMed: 24933654
2. Horton JK et al. Requirement for NBS1 in the S phase checkpoint response to DNA methylation combined with PARP inhibition. DNA Repair (Amst) 10:225-34 (2011). WB ; Human .PubMed: 21130714
3. Reiman A et al. Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours. Br J Cancer105:586-91 (2011). WB ; Human .PubMed: 21792198
4. Weston VJ et al. The PARP inhibitor olaparib induces significant killing of ATM-deficient lymphoid tumor cells in vitro and in vivo. Blood 116:4578-87 (2010). WB ; Human .PubMed: 20739657
Detection of human NBS1 in nuclear extracts from HeLa cells and 293T cells by Western blotting and from HeLa cells by immunoprecipitation with Rabbit anti Human NBS1 (SP2109P) NBS1 was also immunoprecipitated with an antibody directed to an alternate regoin of the molecule
NBN monoclonal antibody (M01), clone 3E4 Western Blot analysis of NBN expression in COLO 320 HSR ( Cat # L020V1 ).
NBN monoclonal antibody (M01), clone 3E4 Western Blot analysis of NBN expression in HL-60 ( Cat # L014V1 ).
Detection limit for recombinant GST tagged NBN is approximately 3ng/ml as a capture antibody.
Western blot analysis of extracts from Hela cells untreated or treated with Hydroxyurea using p95/NBS1 (phospho-Ser343) antibody Cat.-No. AP02356PU
Figure 1. Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using p95/NBS1 antibody (AP02599PU).
Figure 2. Western blot analysis of extracts from Jurkat cells using p95/NBS1 antibody (AP02599PU: Lane 1 and 2) and p95/NBS1 pSer343 antibody (AP02356PU: Lane 3 and 4).
Immunohistochemistry (IHC) analyzes of Nibrin antibody (Cat.-No.: AP06274PU-N) on paraffin-embedded sections.
Western blot (WB) analysis of p95/NBS1 antibody (Cat.-No.: (Cat.-No.: AP06274PU-N) in extracts from 293 cells.
Immunohistochemistry (IHC) analyzes of Nibrin antibody (Cat.-No.: AP06799PU-N) in paraffin-embedded human testis tissue.
Western blot (WB) analysis of Nibrin antibody (Cat.-No.: AP06799PU-N) in extracts from A549 cells.
Immunohistochemistry analysis of p95/NBS1 pSer343 antibody Cat.-No AP01891PU-N in paraffin-embedded human tonsil tissue.
Immunohistochemistry (IHC) analyzes of p-p95/NBS1 (pSer343) antibody (Cat.-No.: AP01891PU-N) in paraffin-embedded human tonsil tissue.
Western blot analysis of p95/NBS1 pSer343 antibody (Cat.-No AP01891PU-N) in extracts from HeLa cells.
Western blot analysis of p95/NBS1 pSer343 antibody (Cat.-No AP01891PU-N) at 1/500 dilution:
Lane 1: Jurkat whole cell lysate.
Lane 2: LO2 whole cell lysate treated with UV.
Lane 3: Raw264.7 whole cell lysate treated with UV.
Lane 4: PC12 whole cell lysate tresated with UV.
Lane 1: Jurkat whole cell lysate.
Lane 2: LO2 whole cell lysate treated with UV.
Lane 3: Raw264.7 whole cell lysate treated with UV.
Lane 4: PC12 whole cell lysate tresated with UV.
Immunofluorescence analysis of A549 cells using Nibrin antibody (Cat.-No AP20504PU-N). The picture on the right is treated with the synthetized peptide.
Western blot analysis of Nibrin antibody (Cat.-No AP20504PU-N) in extracts from Jurkat cells.
Confocal immunofluorescent analysis of Nibrin Antibody (C-term) Cat.-No AP52817PU-N with Hela cell followed by Alexa Fluor®488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of Nibrin Antibody (C-term) Cat.-No AP52817PU-N in NCI-H460, NCI-H292 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells using Nibrin Antibody (C-term) Cat.-No AP52817PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence microscopy of Hela cells labeled with anti-NBS 1 (poly6138) (red). a-tubulin (clone 10D8) was also labeled (green); nuclei were stained with DAPI (blue).View Protocol
Immunohistochemical analysis of Nibrin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Nibrin expression in HeLa (A); HL60 (B) whole cell lysates.
Western blot analysis of Nibrin (pS343) expression in Jurkat (A); LO2 UV-treated (B); Raw264.7 UV-treated (C); PC12 (D) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
ELISA: NBS1 Antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801215)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801215)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801215)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801215)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801215)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801215)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801216)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801216)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801216)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801216)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801216)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801216)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801217)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801217)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801217)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801218)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801218)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801218)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801218)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801218)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801218)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801218)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801218)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801220)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801251)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801251)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801251)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801251)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801251)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801251)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801251)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801251)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801388)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801388)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801388)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801388)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801389)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801389)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801389)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801389)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801390)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801390)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801390)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801390)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801422)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801422)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801424)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801424)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801424)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801448)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801448)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801448)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801448)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801448)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801448)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801448)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801448)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801520)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801520)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801520)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801520)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801520)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801520)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801524)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801525)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801525)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801525)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801525)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801525)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801525)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801525)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801525)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801525)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801553)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NBN mouse monoclonal antibody (UUM800029). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NBN mouse monoclonal antibody (UM800030). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
Detection of human NBS1
Western blot analysis of extracts from HT-29 cells and A549 cells, using NBN antibody.The lane on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm. Positive control: Human lung and breast cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA323004 (NBN Antibody) at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA323004 (NBN Antibody) at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: ×200)
Predicted cell location: Cytoplasm. Positive control: Human lung and breast cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA323004 (NBN Antibody) at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA323004 (NBN Antibody) at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: ×200)
Predicted cell location: Cytoplasm. Positive control: Human liver and gastric cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using NBN antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human liver and gastric cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using NBN antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
Western blot analysis of p95/NBS1 expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of NIBRIN in mouse lung tissue lysate with NIBRIN antibody at 1 ug/mL.
Immunohistochemistry of NIBRIN (CT) in human lung tissue with NIBRIN (CT) antibody at 2.5 ug/mL.
Immunofluorescence of NIBRIN in human lung tissue with NIBRIN antibody at 20 ug/mL.
Western blot analysis of NIBRIN in rat lung tissue lysate with NIBRIN antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry of NIBRIN (NT) in human lung tissue with NIBRIN (NT) antibody at 2.5 ug/mL.
Immunofluorescence of NIBRIN in human lung tissue with NIBRIN antibody at 20 ug/mL.
Western Blot: NBS1 Antibody [TA336720] - NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5).
Immunohistochemistry-Paraffin: NBS1 Antibody [TA336720] - Immunohistochemical staining of paraffin-embedded mouse bone marrow.
Western Blot: NBS1 Antibody [TA336721] - Western Blot on NIH/3T3 (Lane 6) and HeLa whole cell extract (Lane 7).
Immunohistochemistry-Paraffin: NBS1 Antibody [TA336721] - Staining of paraffin-embedded mouse skin.
Western blot analysis on HuvEc cell lysate using Nibrin Antibody
Western blot analysis of HeLa (lane 1), Jurkat (lane 2), MCF-7 (lane 3) and NIH3T3 (lane 4). Anti-NBS1 antibody (clone Poly6138) was used, followed by HRP anti-Rabbit IgG antibody. Beta-actin (clone Poly6221) was used as a loading control.
Immunofluorescence microscopy of HeLa cells labeled with anti-NBS 1 (poly6138) (red). a-tubulin (clone 10D8) was also labeled (green); nuclei were stained with DAPI (blue).
Western blot analysis on HuvEc cell lysate using Phospho-Nibrin(Ser278) Antibody
Western blot analysis of p95/NBS1 phosphorylation expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA327951 | Nibrin (C-term) antibody |
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| Rabbit Polyclonal anti-NBS1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.2 ml /
€255.00
|
|
| OriGene Technologies, Inc. | |||||||
Published recently! |
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| UM800029 | Nibrin antibody |
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| NBN mouse monoclonal antibody,clone UMAB99 | |||||||
 |
Mouse | IgG1 | UMAB99 | Purified | Hu, Mky | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Highly validated antibody!
|
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| UM800030 | Nibrin antibody |
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| NBN mouse monoclonal antibody,clone UMAB100 | |||||||
 |
Mouse | IgG1 | UMAB100 | Purified | Hu, Mky | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
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| UM870029 | Nibrin antibody |
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| NBN mouse monoclonal antibody,clone UMAB99 | |||||||
|
Mouse | IgG1 | UMAB99 | Purified | Hu, Mky | P |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| UM870030 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody,clone UMAB100 | |||||||
|
Mouse | IgG1 | UMAB100 | Purified | Hu, Mky | P |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
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| TA801215 | Nibrin antibody |
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| NBN mouse monoclonal antibody, clone OTI1F11 (formerly 1F11) | |||||||
 |
Mouse | IgG1 | OTI1F11 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA801216 | Nibrin antibody |
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| NBN mouse monoclonal antibody, clone OTI2E9 (formerly 2E9) | |||||||
 |
Mouse | IgG1 | OTI2E9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA801217 | Nibrin antibody |
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| NBN mouse monoclonal antibody, clone OTI1G10 (formerly 1G10) | |||||||
 |
Mouse | IgG1 | OTI1G10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA801218 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTI3A2 (formerly 3A2) | |||||||
 |
Mouse | IgG1 | OTI3A2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA801251 | Nibrin antibody |
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| NBN (p95 NBS1 ) mouse monoclonal antibody, clone OTI3A9 (formerly 3A9) | |||||||
 |
Mouse | IgG2a | OTI3A9 | Purified | Hu | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA801388 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTI8E6 (formerly 8E6) | |||||||
 |
Mouse | IgG1 | OTI8E6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA801389 | Nibrin antibody |
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| NBN mouse monoclonal antibody, clone OTI6D10 (formerly 6D10) | |||||||
 |
Mouse | IgG1 | OTI6D10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA801390 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTI2G4 (formerly 2G4) | |||||||
 |
Mouse | IgG1 | OTI2G4 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA801410 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTIE7 (formerly E7) | |||||||
 |
Mouse | IgG1 | OTIE7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +19 additional images |
Highly validated antibody!
|
||||||
| TA801421 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) | |||||||
 |
Mouse | IgG2b | OTI2F5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
||||||
| TA801424 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTI5E11 (formerly 5E11) | |||||||
 |
Mouse | IgG1 | OTI5E11 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA801427 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTI2E10 (formerly 2E10) | |||||||
 |
Mouse | IgG1 | OTI2E10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
||||||
| TA801448 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTI3C12 (formerly 3C12) | |||||||
 |
Mouse | IgG2a | OTI3C12 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA801520 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTI9H2 (formerly 9H2) | |||||||
 |
Mouse | IgG1 | OTI9H2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA801524 | Nibrin antibody |
||||||
| NBN mouse monoclonal antibody, clone OTIH7 (formerly H7) | |||||||
 |
Mouse | IgG1 | OTIH7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +15 additional images |
Highly validated antibody!
|
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