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Nidogen-1 antibody

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Principal name

Nidogen-1 antibody

Alternative names for Nidogen-1 antibody

NID1, NID, Entactin

SwissProt ID

P08460 (Rat), P10493 (Mouse), P14543 (Human)

Gene ID

18073 (Nid1), 4811 (NID1)

Ncbi ID

NP_035047.2, 9606, NP_002499, NP_002499.2, NM_002508, NM_010917

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat)

Available hosts

Rabbit, Rat, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of Nidogen-1 antibody

Nidogen-1(NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans(heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it(along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.

AP02274SU-S - Nidogen-1
Western Blot analysis of Nidogen 1 antibody Cat.-No AP02274SU specificity. Protein extracts of Heart lung and Skeletal muscle from normal (+/+) and Nidogen 2-mutant (-/-) mice were separated by SDS-PAGE and immunoblotted with AP02274SU (1/1000). This antibody detects several Nidogen 1 specific bands (arrow heads) in normal and Nidogen 2 deficient mice Heart (H), Lung (L), Muscle (M). Schymeinsky J et al. (2002) Mol Cell Biol 22(19): 6820-6830.
AP02274SU-S - Nidogen-1
Immunofluorescence staining in Cryosection of Mouse cartilage and surounding tissue using Nidogen 1 antibody Cat.-No AP02274SU. Schymeinsky J et al. (2002) Mol Cell Biol 22(19): 6820-6830.
H00004811-M01 - Nidogen-1
Western Blot analysis of NID1 expression in transfected 293T cell line by NID1 monoclonal antibody (M01), clone 1G3.Lane 1: NID1 transfected lysate(122.1 KDa).Lane 2: Non-transfected lysate.
H00004811-B01P - Nidogen-1
NID1 MaxPab polyclonal antibody. Western Blot analysis of NID1 expression in human pancreas.
H00004811-B01P - Nidogen-1
Western Blot analysis of NID1 expression in transfected 293T cell line (H00004811-T01) by NID1 MaxPab polyclonal antibody.Lane 1: NID1 transfected lysate(122.54 KDa).Lane 2: Non-transfected lysate.
H00004811-D01P - Nidogen-1
NID1 MaxPab rabbit polyclonal antibody. Western Blot analysis of NID1 expression in mouse lung.
H00004811-D01P - Nidogen-1
NID1 MaxPab rabbit polyclonal antibody. Western Blot analysis of NID1 expression in human kidney.
H00004811-D01P - Nidogen-1
Western Blot analysis of NID1 expression in transfected 293T cell line (H00004811-T02) by NID1 MaxPab polyclonal antibody.Lane 1: NID1 transfected lysate(122.10 KDa).Lane 2: Non-transfected lysate.
C17049-1 - Nidogen-1
Western blot analysis of extracts from K562 cells, using NID1 Antibody. The lane on the right is treated with the synthesized peptide.
CEK1295 - Human Nidogen 1 ELISA Kit
Representative Standard Curve
TP313422 - Nidogen-1

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nidogen-1 antibody
Nidogen-1 Rat IgG2a ELM-1 Purified Ms C, ICC/IF, WB
0.1 mg / €550.00
  OriGene Technologies GmbH

Nidogen-1 antibody
Western Blot analysis of Nidogen 1 antibody Cat.-No AP02274SU specificity. Protein extracts of Heart lung and Skeletal muscle from normal (+/+) and Nidogen 2-mutant (-/-) mice were separated by SDS-PAGE and immunoblotted with AP02274SU (1/1000). This antibody detects several Nidogen 1 specific bands (arrow heads) in normal and Nidogen 2 deficient mice Heart (H), Lung (L), Muscle (M). Schymeinsky J et al. (2002) Mol Cell Biol 22(19): 6820-6830. Rabbit Serum Ms C, E, ICC/IF, R, WB
0.1 ml / €530.00
  OriGene Technologies GmbH
+1 additional image

Nidogen-1 antibody
Western Blot analysis of Nidogen 1 antibody Cat.-No AP02274SU specificity. Protein extracts of Heart lung and Skeletal muscle from normal (+/+) and Nidogen 2-mutant (-/-) mice were separated by SDS-PAGE and immunoblotted with AP02274SU (1/1000). This antibody detects several Nidogen 1 specific bands (arrow heads) in normal and Nidogen 2 deficient mice Heart (H), Lung (L), Muscle (M). Schymeinsky J et al. (2002) Mol Cell Biol 22(19): 6820-6830. Rabbit Serum Ms C, E, ICC/IF, R, WB
20 µl / €180.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Nidogen-1
  Recombinant protein of human nidogen 1 (NID1)  
Nidogen-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.
 

ELISA Kits

Catalog No.    

Human Nidogen-1/Entactin/NID-1 ELISA Kit
Human Nidogen-1/Entactin/NID-1 ELISA Kit  
1 kit / €500.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

NID1 overexpression lysate
NID1 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NID1 (Myc-DDK tagged) - Human nidogen 1 (NID1) , 200 uL, >10^7 TU/mL
Lenti ORF particles, NID1 (Myc-DDK tagged) - Human nidogen 1 (NID1) , 200 uL, >10^7 TU/mL  
200 µl / €2,330.00
  OriGene Technologies, Inc.
 

7 Item(s)

per page

Available Filters

Product Type
  1. ELISA Kit (1)
  2. Human Lenti ORF Particle (1)
  3. Lysate (1)
  4. Primary Antibody (3)
  5. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (2)
  2. Monoclonal (1)
Target
  1. Nidogen-1 (4)
Reactivity
  1. Mouse (Ms) (3)
  2. Human (Hu) (1)
Applications
  1. Frozen sections (C) (3)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  3. Western blot / Immunoblot (WB) (3)
  4. Enzyme Immunoassay (E) (2)
  5. Radioimmunoassay (R) (2)
Presentation
  1. Serum (2)
  2. Purified (1)
Host
  1. Rabbit (2)
  2. Rat (1)
Images
  1. 1 image (3)
Images: Applications
  1. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  2. Western blot / Immunoblot (WB) (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (7)
Service
  • OriGene Technologies GmbH
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