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NKRF antibody

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Principal name

NKRF antibody

Alternative names for NKRF antibody

ITBA4, NRF, NF-kappa-B-repressing, factorTranscription factor NRF, ITBA4 protein

SwissProt ID

O15226 (Human), Q8BY02 (Mouse)

Gene ID

55922 (NKRF), 77286 (Nkrf)

Ncbi ID

NP_060014, 9606, NP_001166958.1, NP_084167.2, NM_029891, NM_017544, NM_001173488

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of NKRF antibody

NKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. NKRF localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm.This gene encodes a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. This gene encodes a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm.

General readings

Dreikhausen,U., et al., (2005) Mol. Cell. Biol. 25 (17), 7473-7483

ARP39289_T100 - NKRF
Human HepG2; WB Suggested Anti-NKRF Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; NKRF antibody - N-terminal region (ARP39289_T100) in Human HepG2 cells using Western Blot
H00055922-M06 - NKRF
Western Blot analysis of NKRF expression in transfected 293T cell line by NKRF monoclonal antibody (M06), clone 1F6.Lane 1: NKRF transfected lysate(77.7 KDa).Lane 2: Non-transfected lysate.
H00055922-B01 - NKRF
Western Blot analysis of NKRF expression in transfected 293T cell line (H00055922-T01) by NKRF MaxPab polyclonal antibody.Lane 1: NKRF transfected lysate(75.9 KDa).Lane 2: Non-transfected lysate.
H00055922-B01P - NKRF
Western Blot analysis of NKRF expression in transfected 293T cell line (H00055922-T02) by NKRF MaxPab polyclonal antibody.Lane 1: NKRF transfected lysate(75.9 KDa).Lane 2: Non-transfected lysate.
ARP39290_P050 - NKRF
Human Breast; Immunohistochemistry with Human Breast tissue at an antibody concentration of 5.0ug/ml using anti-NKRF antibody (ARP39290_P050); NKRF antibody - middle region (ARP39290_P050) in Human Breast cells using Immunohistochemistry
ARP39290_P050 - NKRF
Human Muscle; WB Suggested Anti-NKRF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; NKRF antibody - middle region (ARP39290_P050) in Human Muscle cells using Western Blot
AP17593PU-N - NKRF
Western blot analysis of NKRF antibody (Center) (AP17593PU-N) in Jurkat cell line lysates (35ug/lane). NKRF (arrow) was detected using the purified Pab.
ARP35929_P050 - NKRF
Transfected 293T; WB Suggested Anti-NKRF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; NKRF antibody - N-terminal region (ARP35929_P050) in Transfected 293T cells using Western Blot
ARP35930_P050 - NKRF
Transfected 293T; WB Suggested Anti-NKRF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; NKRF antibody - N-terminal region (ARP35930_P050) in Transfected 293T cells using Western Blot
TA806097 - NKRF
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKRF (RC208507, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKRF.
TP308507 - NKRF
TA339496 - NKRF
WB Suggested Anti-NKRF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T
TA339497 - NKRF
WB Suggested Anti-NKRF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T
TA345220 - NKRF
WB Suggested Anti-NKRF Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate
TA345221 - NKRF
WB Suggested Anti-NKRF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle
TA345221 - NKRF
Immunohistochemistry with Human Breast tissue at an antibody concentration of 5.0 ug/ml using anti-NKRF antibody

9 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NKRF antibody
  NKRF mouse monoclonal antibody, clone OTI4C2 (formerly 4C2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKRF (RC208507, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKRF. Mouse IgG2a OTI4C2 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

NKRF (Center) antibody
Western blot analysis of NKRF antibody (Center) (AP17593PU-N) in Jurkat cell line lysates (35ug/lane). NKRF (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

CRISPR

Catalog No. Species Donor Vector  

NKRF - human gene knockout kit via CRISPR
NKRF - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NKRF - human gene knockout kit via CRISPR
NKRF - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NKRF - human gene knockout kit via CRISPR
NKRF - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NKRF - human gene knockout kit via CRISPR
NKRF - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NKRF
  Recombinant protein of human NFKB repressing factor (NKRF)  
NKRF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nkrf (GFP-tagged) - Mouse NF-kappaB repressing factor (Nkrf), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nkrf (GFP-tagged) - Mouse NF-kappaB repressing factor (Nkrf), 200 uL, >10^7 TU/mL  
200 µl / €1,450.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Nkrf (Myc-DDK-tagged) - Mouse NF-kappaB repressing factor (Nkrf), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nkrf (Myc-DDK-tagged) - Mouse NF-kappaB repressing factor (Nkrf), 200 uL, >10^7 TU/mL  
200 µl / €1,310.00
  OriGene Technologies, Inc.
 

9 Item(s)

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Available Filters

Product Type
  1. CRISPR (4)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (2)
  4. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (1)
  2. Polyclonal (1)
Target
  1. NKRF (3)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (2)
Applications
  1. Western blot / Immunoblot (WB) (2)
Presentation
  1. Purified (1)
Host
  1. Mouse (1)
  2. Rabbit (1)
Images
  1. 1 image (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (9)
Service
  • OriGene Technologies GmbH
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