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NKX2-2 antibody

Principal name

NKX2-2 antibody

Alternative names for NKX2-2 antibody

NKX2.2, NKX2B, Homeobox protein Nkx-2.2, NK2 transcription factor related, locus 2

SwissProt ID

O95096 (Human), P42586 (Mouse), P43697 (Mesau)

Gene ID

18088 (Nkx2-2), 4821 (NKX2-2)

Ncbi ID

NP_035049, 9606, NP_002500, NP_002500.1, ABZ92170.1, NM_002509, NP_035049.1, NM_010919, NM_001077632

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of NKX2-2 antibody

The NKX families of homeobox genes are known to act as intermediaries in the neural response to sonic hedgehog signaling during central nervous system development. NKX2.2 is a member of this family of transcription factors and is necessary for neuroendocrine differentiation in the central nervous system and pancreas (Sander M, 2000). This protein was recently identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It also acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors (Yoshida A, 2012). NKX2.2 preferentially binds the promoter of the αcell-specific factor Arx in βcells when the promoter is in a hypermethylated state. Disruption of the interaction between NKX2.2 and Grgs in mice leads to ectopic expression of Arx in βcells and is sufficient to cause β-to-α-cell reprogramming during embryonic development and in the adult. These combined in vitro and in vivo studies elucidate how cell-specific DNA modifications and transcriptional regulatory events come together to establish and maintain islet βcell identity (Papizan JB, 2011).

General readings

Wang, Y.C. et al., J. Surg. Res. 163(1): 47-51 (2010)
Wang, Y.C. et al., Endocr. Relat. Cancer 16(1): 267-279 (2009)
Chen, M. et al., J. Biol. Chem. 284(3): 1484-1494 (2009)
Owen, L.A. et al., PLoS ONE 3 (4): e1965 (2008)
Pauls, S. et al., Dev. Biol. 304(2): 875-890 (2007)

Mouse SP2/0; WB Suggested Anti-NKX2-2 Antibody Titration: 0.125ug/ml. ELISA Titer: 1:1562500. Positive Control: SP2/0 cell lysate; NKX2-2 antibody - N-terminal region (ARP37110_P050) in Mouse SP2/0 cells using Western Blot

ARP37110_P050

Hum. Fetal Brain; NKX2-2 antibody - N-terminal region (ARP38167_P050) in Hum. Fetal Brain cells using Western Blot

ARP38167_P050

Human Placenta; WB Suggested Anti-NKX2-2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; NKX2-2 antibody - N-terminal region (ARP38167_P050) in Human Placenta cells using Western Blot

ARP38167_P050

Human; . Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage CellsPrimary. Dilution:. IHC-PZ 1:500, IHC-P 1:1000; NKX2-2 antibody - N-terminal region (ARP32338_P050) in Human cells using Immunohistochemistry

ARP32338_P050

Human Spleen; WB Suggested Anti-NKX2-2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Spleen; NKX2-2 antibody - N-terminal region (ARP32338_P050) in Human Spleen cells using Western Blot

ARP32338_P050

Western blot analysis in 293 cell line lysates (35ug/lane) using NKX2-2 antibody Cat.-No. AP52892PU-N. This demonstrates this antibody detected the NKX2-2 protein (arrow).

AP52892PU-N

Western blot analysis using NKX2.2 antibody Cat.-No AM06771PU-N against human NKX2.2 recombinant protein (Expected MW is 22 kDa)

AM06771PU-N

AM06771PU-N

Flow cytometric analysis of MCF-7 cells using NKX2.2 antibody Cat.-No AM06771PU-N (green) and negative control (red).

AM06771PU-N

Western blot analysis in rat kidney tissue lysate with NKX2-2 antibody Cat.-No. AP55626PU-N at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

AP55626CP-N

Formalin-Fixed, Paraffin-Embedded Human Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM32850PU (Clone NX2/294).

Formalin-Fixed, Paraffin-Embedded Human Pancreas stained with NKX2.2 Antibody Cat.-No AM32850PU (Clone NX2/294).

Formalin-paraffin Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM50148PU (Clone SPM564).

Formalin-paraffin pancreas stained with NKX2.2 Antibody Cat.-No AM50148PU (Clone SPM564).

TP760219

Western blot analysis of NKX2-2 in rat kidney tissue lysate with NKX2-2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

TA319689

Immunohistochemistry of NKX2-2 in human kidney tissue with NKX2-2 antibody at 2.5 ug/mL.

TA319689

Immunofluorescence of NKX2-2 in human kidney tissue with NKX2-2 antibody at 20 ug/mL.

TA319689

Host: Rabbit; Target Name: NKX2-2; Sample Tissue: Jurkat Whole Cell lysates; Antibody Dilution: 1.0ug/ml

TA329799

WB Suggested Anti-NKX2-2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Spleen

TA330256

Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage CellsPrimary; Dilution: IHC-PZ 1:500, IHC-P 1:1000

TA330256

WB Suggested Anti-NKX2-2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Placenta

TA335820

Hum. Fetal Brain

TA335820

WB Suggested Anti-NKX2-2 Antibody Titration: 0.125 ug/ml; ELISA Titer: 1:1562500; Positive Control: SP2/0 cell lysate

TA342285

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AM50148PU-S	NKX2-2 antibody
	Mouse	IgG2b	SPM564	Purified	Chk, Hu, Ms, Rt	F, ICC/IF, P	0.1 mg / €430.00
							OriGene Technologies GmbH
+1 additional image	OriGene recommends this product for Immunohistochemistry.
AM50148PU-T	NKX2-2 antibody
	Mouse	IgG2b	SPM564	Purified	Chk, Hu, Ms, Rt	F, ICC/IF, P	20 µg / €240.00
							OriGene Technologies GmbH
+1 additional image	OriGene recommends this product for Immunohistochemistry.
TA319689	NKX2-2 (Center) antibody
	Rabbit Polyclonal NKX2-2 Antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms, Rt	E, ICC/IF, P, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
+2 additional images
AM06771PU-N	NKX2-2 antibody
	Mouse	IgG1	3E4	Purified	Hu	E, F, WB	0.1 mg / €410.00
							OriGene Technologies GmbH
+2 additional images
AP52892PU-N	NKX2-2 (Center) antibody
	Rabbit	Ig		Aff - Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP760219	NKX2-2 (full length, N-term HIS tag)
	Recombinant protein of human NK2 homeobox 2 (NKX2-2) , full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
	Human	> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY419283	NKX2 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR227494L1V	Lenti ORF particles, Nkx2 (Myc-DDK-tagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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