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NLK antibody

Principal name

NLK antibody

Alternative names for NLK antibody

LAK1, Nemo-like kinase, Serine/threonine protein kinase NLK

SwissProt ID

O54949 (Mouse), Q9UBE8 (Human)

Gene ID

51701 (NLK), 18099 (Nlk)

Ncbi ID

NP_057315.3, NM_016231, NP_057315.2, NP_057315, NP_032728.3, NM_008702, BC057667

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NLK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Detection limit for recombinant GST tagged NLK is approximately 0.03ng/ml as a capture antibody.

H00051701-M01

Formalin-fixed and paraffin-embedded human cancer tissue reacted with NLK polyclonal antibody ( Cat # PAB2944 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

PAB2944

Western blot analysis of NLK polyclonal antibody ( Cat # PAB2944 ) in A-375 cell lysate. NLK ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

PAB2944

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and NLK HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-NLK mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00051701-M02

NLK monoclonal antibody (M02), clone 2B11. Western Blot analysis of NLK expression in NIH/3T3(Cat # L018V1 ).

H00051701-M02

NLK monoclonal antibody (M02), clone 2B11 Western Blot analysis of NLK expression in HepG2 ( Cat # L019V1 ).

H00051701-M02

NLK monoclonal antibody (M02), clone 2B11. Western Blot analysis of NLK expression in PC-12(Cat # L012V1 ).

H00051701-M02

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

AP14183PU-N

Western blot analysis of anti-NLK Pab in A375 cell lysate. NLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

AP14183PU-N

Human 293T; WB Suggested Anti-NLK Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate; NLK antibody - middle region (ARP56863_P050) in Human 293T cells using Western Blot

ARP56863_P050

TP308723

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA309011 diluted at 1:1000.

TA309011

Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. NLK antibody. TA309011 diluted at 1:1000.

TA309011

NLK antibody [N3C3] detects NLK protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. NLK antibody [N3C3] (TA309011) dilution: 1:500.

TA309011

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NLK(TA309011) antibody at 1:100 dilution.

TA309011

NLK Antibody(Cat. #TA324850) western blot analysis in SiHa,Hela cell line lysates (35ug/lane).This demonstrates the NLK antibody detected the NLK protein (arrow).

TA324850

Western blot analysis of extracts of HeLa cell line, using NLK antibody.

TA332581

WB Suggested Anti-NLK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 293T cell lysate

TA345050

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA309011	NLK antibody
	Rabbit Polyclonal antibody to NLK (nemo-like kinase)
	Rabbit	IgG		Aff - Purified	Hu, Ms	ICC/IF, WB	0.1 ml / €415.00
							OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
TA324850	NLK antibody
	Mouse monoclonal NLK Antibody
	Mouse	IgG2a	1146CT24.2.1	Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies, Inc.
TA332581	NLK antibody
	Rabbit anti-NLK Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
AP14183PU-N	NLK (C-term) antibody
	Rabbit	Ig		Purified	Hu	P, WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP308723	NLK
	Recombinant protein of human nemo-like kinase (NLK)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR208263L2V	Lenti ORF particles, Nlk (GFP-tagged) - Mouse nemo like kinase (cDNA clone MGC:68238 IMAGE:4235238), 200 uL, >10^7 TU/mL
		200 µl / €1,240.00
		OriGene Technologies, Inc.

MR208261L2V	Lenti ORF particles, Nlk (GFP-tagged) - Mouse nemo like kinase (Nlk), 200 uL, >10^7 TU/mL
		200 µl / €1,220.00
		OriGene Technologies, Inc.

MR208263L1V	Lenti ORF particles, Nlk (Myc-DDK-tagged) - Mouse nemo like kinase (cDNA clone MGC:68238 IMAGE:4235238), 200 uL, >10^7 TU/mL
		200 µl / €1,110.00
		OriGene Technologies, Inc.

MR208261L1V	Lenti ORF particles, Nlk (Myc-DDK-tagged) - Mouse nemo like kinase (Nlk), 200 uL, >10^7 TU/mL
		200 µl / €1,090.00
		OriGene Technologies, Inc.

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