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NMDA Receptor 2A antibody

Principal name

NMDA Receptor 2A antibody

Alternative names for NMDA Receptor 2A antibody

NMDAR2A, GRIN2A, NR2A, Glutamate [NMDA] Receptor subunit epsilon-1

SwissProt ID

P35436 (Mouse), Q00959 (Rat), Q12879 (Human)

Gene ID

24409 (Grin2a), 2903 (GRIN2A), 14811

Ncbi ID

10116, NP_000824, NP_000824.1, NP_036705, NP_036705.3, NP_032196.2, NM_008170, NM_001134407

Available reactivities

Bov (Bovine), Ms (Mouse), Rt (Rat), Hu (Human), Bt (Bat), Can (Canine), Chk (Chicken), Hst (Hamster), Mky (Monkey), Op (Opossum), Prim (Primate), Xen (Xenopus), Tr (Turkey), Bear, Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of NMDA Receptor 2A antibody

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kaite receptors. The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functiol NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit. The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neurol depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels. Ca2+ entry through the NMDAR regulates numerous downstream sigling pathways including long term potentiation (a molecular model of memory) and syptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimerâ??s disease. NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the sypses and interlization as well as phosphorylation and interaction with other intracellular proteins.

General readings

Endele, S., et al. Nat. Genet. 42(11):1021-1026(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Saus, E., et al. J Psychiatr Res 44(14):971-978(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010)
King, J.E., et al. Am. J. Pathol. 176(6):2819-2830(2010)

Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2a subunit of the NMDA receptor. Using Grin2a polyclonal antibody ( Cat # PAB9635 ).

PAB9635

Western blot of 10 µg of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k Grin2a subunit of the NMDA receptor. Using Grin2a polyclonal antibody ( Cat # PAB9699 ).

PAB9699

Rat hippocampal; . Sample Type :. Rat Hippocampal Neurons - 14DIV. Primary Antibody Dilution :. 1:200. Secondary Antibody :. Anti-rabbit-Cy3. Secondary Antibody Dilution :. 1:500. Color/Signal Descriptions :. Green: GFP Red: NR2a Yellow: VGLUT12. Gene Name :. GRIN2A. Submitted by :. Dan Fowler - University of Oregon, Institute of Neuroscience; GRIN2A antibody - middle region (ARP35012_P050) in Rat hippocampal cells using Immunohistochemistry

ARP35012_P050

Human Muscle; WB Suggested Anti-GRIN2A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; GRIN2A antibody - middle region (ARP35012_P050) in Human Muscle cells using Western Blot

ARP35012_P050

Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR2A/B (Phospho-Tyr1246/1252) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using NMDAR2A/B (Phospho-Tyr1246/1252) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NMDAR2A/B (Ab-1246/1252) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using NMDAR2A/B (Ab-1246/1252) Antibody. The picture on the right is treated with the synthesized peptide.

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23982PU-N

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23982PU-N

Western blot of Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDAR phosphorylated at Tyr1325 (Control). The phosphospecificity of this labeling is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).

AP31638PU-N

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with NMDA Receptor 2A Antibody (Center) Cat.-No AP51952PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.

AP51952PU-N

Western blot analysis of NMDA Receptor 2A Antibody (Center) Cat.-No AP51952PU-N in mouse heart tissue lysates (35ug/lane).

AP51952PU-N

Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue reacted with NMDA Receptor 2A Antibody (C-term) Cat.-No AP51953PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.

AP51953PU-N

Western blot analysis of NMDA Receptor 2A Antibody (C-term) Cat.-No AP51953PU-N in MDA-MB435 cell line lysates (35ug/lane).

AP51953PU-N

Flow cytometric analysis of MDA-MB435 cells using NMDA Receptor 2A Antibody (C-term) Cat.-No AP51953PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AP51953PU-N

Immunostaining of rabbit retina showing NR2A in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.

AP26445PU-N

Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k NR2A subunit of the NDMA receptor.

AP26445PU-N

Adult rat hippocampus immunohistochemstry

75-288

Adult rat brain membrane immunoblot

75-288

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged NR2A, NR2B or untagged Kv2.1 plasmid and probed with N327/95 (left) and N86/38 (right) TC supe.

75-288

Adult rat hippocampus immunohistochemstry

73-288

Adult rat brain membrane immunoblot

73-288

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged NR2A, NR2B or untagged Kv2.1 plasmid and probed with N327/95 (left) and N86/38 (right) TC supe.

73-288

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged NR2A, NR2B or untagged Kv2.1 plasmid and probed with N327A/38 (left) and N86/38 (right) TC supe.

73-303

Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.

TA309187

Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.

TA309188

Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.

TA309189

Western blot analysis of rat brain lysates: 1. Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody, (1:600). 2. Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody, preincubated with the control peptide antigen.

TA328821

IHC staining of mouse brain sections using Anti-GluN2B (extracellular) antibody , (1:60) and Anti-GluN2A (extracellular) antibody, (1:200). A. Sections were incubated with Anti-GluN2A (extracellular) antibody, followed by goat anti-rabbit-Alexa-488 (green). B. The same sections were incubated with Anti-GluN2B (extracellular) antibody (red). C. Merge of A and B demonstrates the ubiquitous colocalization of the GluN2A and GluN2B subunits in cells with neuronal profiles in this nucleus.

TA328821

IHC staining of rat hippocampal dentate gyrus with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody. A. NMDA Receptor 2A (green) appears diffusely in the outer molecular layer of the dentate gyrus (Out Mol.) and in cells along the subgranular layer (arrows). B. Staining of parvalbumin (PV, red) identifies interneurons in the dentate gyrus. C. Confocal merge demonstrates localization of PV in some neurons with NMDA Receptor 2A.

TA328821

Expression of NMDA Receptor 2A in rat C6 glioma cells. Immunocytochemical staining of live intact rat C6 glioma cells. A. Cells were stained with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody, (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Cell nuclei were stained with the cell permeable dye Hoechst 33342 (blue staining). B. Live view of the same field.

TA328821

Immunoprecipitation of rat brain lysates. 1. Cell lysate. 2. Cell lysates + protein A beads + Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody. 3. Cell lysates + protein A beads + pre-immune rabbit serum. Black arrow indicates the NMDA Receptor 2A protein while the red arrow shows the IgG heavy chain. Immunoblot was performed with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody.

TA328821

WB Suggested Anti-GRIN2A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle

TA338510

Sample Type . Rat Hippocampal Neurons - 14DIVPrimary Antibody Dilution . 1:200 Secondary Antibody . Anti-rabbit-Cy3Secondary Antibody Dilution . 1:500Color/Signal Descriptions . Green: GFP Red: NR2a Yellow: VGLUT12Gene Name . GRIN2ASubmitted by . Dan Fowl

TA338510

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (GRIN2A Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA350718

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDAR phosphorylated at Tyr1325 (Control). The phosphospecificity of this labeling is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).

TA309190

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA309187	NMDA Receptor 2A (C-term) antibody
	Rabbit Anti-NMDA NR2A Subunit Antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms, Rt	C, IP, WB	10 µg / €405.00
							OriGene Technologies, Inc.
	Published recently!
TA328821	NMDA Receptor 2A antibody
	Rabbit Polyclonal Anti-NMDA Receptor 2A (GluN2A) (extracellular)
	Rabbit			Purified	Hu, Ms, Rt	WB	0.2 ml / €585.00
							OriGene Technologies, Inc.
+4 additional images	Highly validated antibody!
TA309188	NMDA Receptor 2A (C-term) antibody
	Rabbit Anti-NMDA NR2A Subunit Antibody
	Rabbit	IgG		Serum	Hu, Ms, Rt	WB	50 µl / €355.00
							OriGene Technologies, Inc.
TA309189	NMDA Receptor 2A (N-term) antibody
	Rabbit Anti-NMDA NR2A Subunit, N-terminus Antibody
	Rabbit	IgG		Aff - Purified	Bov, Ms, Rt	C, ICC/IF, WB	0.1 ml / €405.00
							OriGene Technologies, Inc.
TA309190	NMDA Receptor 2A antibody
	Rabbit Anti-NMDA NR2A Subunit (Tyr1325) Antibody (Phospho-Specific)
	Rabbit	IgG		Purified	Ms, Rt	WB	0.1 ml / €405.00
							OriGene Technologies, Inc.
TA326537	NMDA Receptor 2A (75-325, Extracell. Dom.) antibody
	Mouse Monoclonal Anti-GluN2A/NR2A Antibody
	Mouse	IgG2a	S327-95	Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 mg / €410.00
							OriGene Technologies, Inc.
TA326538	NMDA Receptor 2A antibody
	Mouse Monoclonal Anti-GluN2A/NR2A Antibody
	Mouse	IgG2b	S327A-38	Purified	Hu, Ms, Rt	WB	0.1 mg / €410.00
							OriGene Technologies, Inc.
TA350718	NMDA Receptor 2A antibody
	Rabbit Polyclonal Anti-GRIN2A Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
73-288	NMDA Receptor 2A antibody
	Mouse	IgG2a	N327/95	Supernatant	Hu, Ms, Rt	C, WB	5 ml / €490.00
							Antibodies Incorporated
+2 additional images
73-303	NMDA Receptor 2A antibody
	Mouse	IgG2b	N327A/38	Supernatant	Hu, Ms, Rt	C, WB	5 ml / €490.00
							Antibodies Incorporated

75-288	NMDA Receptor 2A antibody
	Mouse	IgG2a	N327/95	Purified	Hu, Ms, Rt	C, WB	0.1 ml / €570.00
							Antibodies Incorporated
+2 additional images
AP26445PU-N	NMDA Receptor 2A (C-term) antibody
	Rabbit	Ig		Aff - Purified	Hu, Ms, Rt	IP, WB	0.1 ml / €405.00
							OriGene Technologies GmbH
+1 additional image
AP51952PU-N	NMDA Receptor 2A (Center) antibody
	Rabbit	Ig		Aff - Purified	Hu, Ms	P, WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image
AP51953PU-N	NMDA Receptor 2A (C-term) antibody
	Rabbit	Ig		Aff - Purified	Hu	F, P, WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+2 additional images

Human Lenti ORF Particles

Catalog No.
RC226397L2V	Lenti ORF particles, GRIN2A (mGFP-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2A (GRIN2A), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €2,680.00
		OriGene Technologies, Inc.

RC226397L1V	Lenti ORF particles, GRIN2A (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2A (GRIN2A), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €2,420.00
		OriGene Technologies, Inc.

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