NNMT antibody
Principal name
NNMT antibody
Alternative names for NNMT antibody
Nicotinamide N-methyltransferase
Ncbi ID
9606, NP_006160, NP_006160.1, NP_035054.1, NM_010924, NM_006169
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of NNMT antibody
- Kit Component:
- - KN311087G1, Nnmt gRNA vector 1 in pCas-Guide vector
- KN311087G2, Nnmt gRNA vector 2 in pCas-Guide vector
- KN311087D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Parsons RB., et al. (2002). J Neuropathol Exp Neurol. 61(2):111-24.
Western Blot analysis of NNMT expression in transfected 293T cell line by NNMT monoclonal antibody (M03), clone 2F2.Lane 1: NNMT transfected lysate(29.6 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged NNMT is approximately 1ng/ml as a capture antibody.
NNMT polyclonal antibody (A01), Lot # CIL0051213QCS1 Western Blot analysis of NNMT expression in A-431 ( Cat # L015V1 ).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with NNMT polyclonal antibody ( Cat # PAB2193 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of NNMT ( arrow ) using rabbit NNMT polyclonal antibody ( Cat # PAB2193 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the NNMT gene ( Lane 2 ) ( Origene Technologies ) .
NNMT MaxPab rabbit polyclonal antibody. Western Blot analysis of NNMT expression in mouse lung.
Western Blot analysis of NNMT expression in transfected 293T cell line (H00004837-T01) by NNMT MaxPab polyclonal antibody.Lane 1: NNMT transfected lysate(29.60 KDa).Lane 2: Non-transfected lysate.
Human HepG2; WB Suggested Anti-NNMT Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; NNMT antibody - N-terminal region (ARP42281_T100) in Human HepG2 cells using Western Blot
NNMT, 1-264 aa human, His-tagged, recombinant: 15% SDS-PAGE (3 µg)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502470)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502470)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502470)
Anti-NNMT mouse monoclonal antibody (TA502470) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT(RC200641).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
HEK293T cells transfected with either RC200641 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NNMT antibody(TA502470), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502521)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502521)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
HEK293T cells transfected with either RC200641 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NNMT antibody(TA502521), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-NNMT antibody(TA502521),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-NNMT antibody(TA502521),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502522)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502522)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502522)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NNMT monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502538)
Anti-NNMT mouse monoclonal antibody (TA502538) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT(RC200641).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
Flow cytometric Analysis of Hela cells, using anti-NNMT antibody(TA502538),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC200641 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NNMT antibody(TA502538), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-NNMT antibody(TA502538),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502624)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502624)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502624)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502624)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502624)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502624)
Anti-NNMT mouse monoclonal antibody (TA502624) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT(RC200641).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NNMT monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
Flow cytometric Analysis of Jurkat cells, using anti-NNMT antibody(TA502624),(Red), compared to a nonspecific negative control antibody,(Blue).
Western blot analysis of NNMT (arrow) using rabbit polyclonal NNMT Antibody (Center)(Cat# TA302022). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NNMT gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with NNMT antibody (Center) (Cat# TA302022), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TA341383 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA341384 (0.1µg/ml) staining of A549 (A) and HeLA (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302022 | NNMT antibody |
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| Rabbit Polyclonal Antibody against NNMT (Center) | |||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA341383 | NNMT antibody |
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| Goat Anti-NNMT (aa171-182) Polyclonal Antibody | |||||||
 |
Goat | Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341384 | NNMT antibody |
||||||
| Goat Anti-NNMT Polyclonal Antibody | |||||||
 |
Goat | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||