Noelin antibody
Principal name
Noelin antibody
Alternative names for Noelin antibody
OLFM1, NOE1, NOEL1, Olfactomedin-1, Neuronal olfactomedin-related ER localized protein
Ncbi ID
9606, 10116, NP_006325, NP_006325.1, NP_001033701.1, NM_019498, NM_001038613, NM_001038614, NM_053573, NM_001038612, NM_014279
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Noelin antibody
- Kit Component:
- - KN311462G1, Olfm1 gRNA vector 1 in pCas-Guide vector
- KN311462G2, Olfm1 gRNA vector 2 in pCas-Guide vector
- KN311462D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Yokoyama M, Nishi Y, Yoshii J, Okubo K, Matsubara K (May 1997). DNA Res 3 (5): 311–20
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with OLFM1 polyclonal antibody ( Cat # PAB2558 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of OLFM1 ( arrow ) using rabbit OLFM1 polyclonal antibody ( Cat # PAB2558 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the OLFM1 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of Olfm1 ( arrow ) using rabbit Olfm1 polyclonal antibody ( Cat # PAB2559 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the Olfm1 gene ( Lane 2 ) ( Origene Technologies ) .
Adult rat whole brain immunohistochemistry
immunoblot versus lysates from adult wild-type (+/+) and Pancortin knockout (-/-) mouse cortex probed with K96/7. Data courtesy of Mark Mattson (NIA/NIH) and reproduced with permission from the Journal of Neuroscience (2007 Cheng et al, PMID 17301160).
Adult rat brain membrane immunoblot
immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K96/7 (left) orN52A/42 (right) TC supe.
Adult rat whole brain immunohistochemistry
immunoblot versus lysates from adult wild-type (+/+) and Pancortin knockout (-/-) mouse cortex probed with K96/7. Data courtesy of Mark Mattson (NIA/NIH) and reproduced with permission from the Journal of Neuroscience (2007 Cheng et al, PMID 17301160).
Adult rat brain membrane immunoblot
immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K96/7 (left) orN52A/42 (right) TC supe.
Western blot analysis of Noelin-1(Olfm1) (arrow) using rabbit polyclonal Noelin-1(Olfm1) Antibody (N-term) (Cat.#TA302007).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Olfm1 Antibody (N-term) (Cat.#TA302007), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of Noelin-1(Olfm1) (arrow) using rabbit polyclonal Noelin-1(Olfm1) Antibody (C-term) (Cat.#TA302008).
Predicted band size: 55 kDa . Positive control: Brain tissue; MCF7, A549 and SW480 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Brain tissue lysate Lane2: MCF7 cell lysate Lane3: A549 cell lysate Lane4: SW480 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Secreted. Positive control: Lung cancer tissue. Recommended dilution: 1/50-200 The image is immunohistochemistry of paraffin-embedded Lung cancer tissue using OLFM1 antibody at dilution 1/50
IHC analysis of Pancortin in frozen sections of mouse brain extract using the antibody
WB Suggested Anti-OLFM1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Brain
WB Suggested Anti-OLFM1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal kidney
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| 73-042 | Noelin antibody |
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Mouse | IgG1 | K96/7 | Supernatant | Hu, Ms, Rt | C, IP, WB |
5 ml /
€490.00
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| Antibodies Incorporated | |||||||
 +3 additional images |
Highly validated antibody!
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| 75-042 | Noelin antibody |
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Mouse | IgG1 | K96/7 | Purified | Hu, Ms, Rt | C, IP, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA302007 | Noelin (N-term) antibody |
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| Rabbit Polyclonal Antibody against OLFM1 (N-term) | |||||||
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA302008 | Noelin (C-term) antibody |
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| Rabbit Polyclonal Antibody against OLFM1 (C-term) | |||||||
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322511 | Noelin antibody |
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| Anti-OLFM1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA326496 | Noelin antibody |
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| Mouse Monoclonal Anti-Pancortin Antibody | |||||||
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Mouse | IgG1 | S96-7 | Purified | Hu, Ms, Rt | C, ICC/IF, WB |
0.1 mg /
€410.00
|
| OriGene Technologies, Inc. | |||||||
Mouse Lenti ORF Particles
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205517L2V | Lenti ORF particles, Olfm1 (GFP-tagged ORF) - Rat olfactomedin 1 (Olfm1), 200 uL, >10^7 TU/mL |
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200 µl /
€1,170.00
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|
| OriGene Technologies, Inc. | ||
| RR205517L1V | Lenti ORF particles, Olfm1 (Myc-DDK-tagged ORF) - Rat olfactomedin 1 (Olfm1), 200 uL, >10^7 TU/mL |
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200 µl /
€1,040.00
|
|
| OriGene Technologies, Inc. | ||