Noggin antibody
Principal name
Noggin antibody
Alternative names for Noggin antibody
NOG
Ncbi ID
NP_005441.1, 9606, NP_005441, NM_005450, NP_032737, NP_032737.1
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)
Background of Noggin antibody
The secreted polypeptide, encoded by this gene, binds and ictivates members of the transforming growth factor-beta (TGF-beta) superfamily sigling proteins,such as bone morphogenetic protein-4 (BMP4).By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily,this protein may have a principal role in creating morphogenic gradients.The protein appears to have pleiotropic effect,both early in development as well as in later stages.It was origilly isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment.The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes,such as neural tube fusion and joint formation. Recently,several domint human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified;both SYM1 and SYNS1 have multiple joint fusion as their principal feature,and map to the same region (17q22) as this gene.All of these mutations altered evolutiorily conserved amino acid residues.The amino acid sequence of this human gene is highly homologous to that of Xenopus,rat and mouse.[provided by RefSeq,Jul 2008]
NOG MaxPab rabbit polyclonal antibody. Western Blot analysis of NOG expression in human liver.
Western Blot analysis of NOG expression in transfected 293T cell line (H00009241-T02) by NOG MaxPab polyclonal antibody.Lane 1: NOG transfected lysate(25.80 KDa).Lane 2: Non-transfected lysate.
NOG MaxPab rabbit polyclonal antibody. Western Blot analysis of NOG expression in Jurkat.
Western blot analysis of NOG in 20 µg of human hippocampus tissue lysate ( Lanes 2 and 4 ) with NOG polyclonal antibody at 1 : 1000 dilution ( Cat# PAB12096 ). Lane1 : MW marker, Lane3 : control.
Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE)
Western Blot analysis of NOG expression in transfected 293T cell line (H00009241-T01) by NOG MaxPab polyclonal antibody.Lane 1: NOG transfected lysate(25.52 KDa).Lane 2: Non-transfected lysate.
NOG MaxPab rabbit polyclonal antibody. Western Blot analysis of NOG expression in human liver.
Western Blot analysis of NOG expression in transfected 293T cell line (H00009241-T02) by NOG MaxPab polyclonal antibody.Lane 1: NOG transfected lysate(25.80 KDa).Lane 2: Non-transfected lysate.
NOG MaxPab rabbit polyclonal antibody. Western Blot analysis of NOG expression in Jurkat.
Immunoprecipitation of NOG transfected lysate using anti-NOG MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NOG MaxPab mouse polyclonal antibody (B01) (H00009241-B01).
Human ACHN; WB Suggested Anti-NOG Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate; NOG antibody - middle region (ARP58773_P050) in Human ACHN cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Nog mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500028)
Immunofluorescent staining of A549 cells using anti-Nog mouse monoclonal antibody (TA500028).
Anti-Nog mouse monoclonal antibody (TA500028) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog(RC205020).
Noggin antibody (4C12) at 1:2000 + Recombinant human Noggin (Cat# RC205020)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Nog monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog (RC205020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Nog mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500029)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-Nog mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500029)
Anti-Nog mouse monoclonal antibody (TA500029) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog(RC205020).
Noggin antibody (1H8) at 1:40000 + Recombinant human Noggin (Cat# RC205020)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog (RC205020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Nog monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-Nog (TA500116) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-Nog (TA500116) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon using anti-Nog (TA500116) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-Nog (TA500116) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-Nog (TA500116) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-Nog(TA500116) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded pancreas within the normal limits using anti-Nog(TA500116) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded thyroid within the normal limits using anti-Nog(TA500116) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Anti-Nog mouse monoclonal antibody (TA500116) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog(RC205020).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog(RC205020) (RC205020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-Nog monoclonal antibody at 1:200 dilution.
Western Blot: Noggin Antibody [TA336497] 20ug of human hippocampus tissue lysate (lanes 2 and 4) with Noggin polyclonal at 1:1000 dilution followed by secondary at 1:5000 dilution MW marker Lane 1 peptide absorption control lane 3.
Western Blot: Noggin Antibody [TA336514] - Western blot detection of Noggin in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Noggin polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Pep
WB Suggested Anti-NOG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: ACHN cell lysate
Western blot detection of Noggin in CHO-K1 cell lysates over-expressing Noggin-PDGFR transmembrane domain fused protein using Noggin mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:37KDa.
Immunocytochemistry staining of HeLa cells surface-expressing Noggin-PDGFR transmembrane domain fused protein using Noggin mouse mAb (dilution 1:100).
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500028 | Noggin antibody |
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| Noggin (Noggin) mouse monoclonal antibody, clone OTI4C12 (formerly 4C12) | |||||||
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Mouse | IgG2b | OTI4C12 | Purified | Can, Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
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| TA500029 | Noggin antibody |
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| Noggin (Noggin) mouse monoclonal antibody, clone OTI1H8 (formerly 1H8) | |||||||
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Mouse | IgG2b | OTI1H8 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA500116 | Noggin antibody |
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| anti-Noggin (Noggin) mouse monoclonal antibody, clone OTI1C1 (formerly 1C1) | |||||||
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Mouse | IgG2b | OTI1C1 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
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| TA336497 | Noggin antibody |
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| Rabbit Polyclonal Noggin Antibody | |||||||
![Western Blot: Noggin Antibody [TA336497] 20ug of human hippocampus tissue lysate (lanes 2 and 4) with Noggin polyclonal at 1:1000 dilution followed by secondary at 1:5000 dilution MW marker Lane 1 peptide absorption control lane 3.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-93547-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA336514 | Noggin antibody |
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| Rabbit Polyclonal Noggin Antibody | |||||||
![Western Blot: Noggin Antibody [TA336514] - Western blot detection of Noggin in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Noggin polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Pep](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB110-40413-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€395.00
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| OriGene Technologies, Inc. | |||||||
| TA347010 | Noggin antibody |
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| Mouse Monoclonal Noggin Antibody | |||||||
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Mouse | IgG3 | 1D2-G8-G1 | Purified | ICC/IF, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP08597PU-N | Noggin antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| GT15159-100 | Noggin antibody |
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Goat | Aff - Purified | Ms | C, E, ICC/IF, WB |
0.1 mg /
€450.00
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| Neuromics Antibodies | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP723333 | Noggin |
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| Purified recombinant protein of Human noggin (NOG). | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€230.00
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| OriGene Technologies, Inc. | |||||
| TP723334 | Noggin |
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| Purified recombinant protein of Mouse noggin (Nog). | |||||
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Mouse | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
20 µg /
€230.00
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| OriGene Technologies, Inc. | |||||
| PR15070CF-50 | Noggin (Fc Chimera) |
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Human | Purified | > 90 % |
50 µg /
€400.00
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| Neuromics Antibodies | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401672 | NOG overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. |