NOTCH2 antibody
Principal name
NOTCH2 antibody
Alternative names for NOTCH2 antibody
Notch 2, hN2, Neurogenic locus notch homolog protein 2
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)
Background of NOTCH2 antibody
NOTCH2 is a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch sigling network is an evolutiorily conserved intercellular sigling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular sigling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. NOTCH2 is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. NOTCH2 functions as a receptor for membrane bound ligands, and may play a role in vascular, rel and hepatic development.This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch sigling network is an evolutiorily conserved intercellular sigling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular sigling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, rel and hepatic development. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.
Formalin-Fixed Paraffin-Embedded Human Kidney stained with NOTCH2 antibody Cat.-No AP07611SU-N
Western blot using anti-Notch 2 (intra) antibody shows detection of a band at ~110 kD corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100 µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2).
Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection.
Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points.
The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1/400 dilution. ECL was used as visualization method.
Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection.
Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points.
The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1/400 dilution. ECL was used as visualization method.
Figure 1. Staining NOTCH2 in Liver by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Immunohistochemical staining with NOTCH2 polyclonal antibody ( Cat # PAB10293 ) was diluted 1 : 500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Immunohistochemical staining with NOTCH2 polyclonal antibody ( Cat # PAB10294 ) was diluted 1 : 500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Western blot using NOTCH2 polyclonal antibody ( Cat # PAB10294 ) ( intra ) antibody shows detection of a band at ~110 kDa correspondingto active NOTCH2 protein ( arrowhead ).Western Blot analysis was performed for NOTCH2 expression using 100 µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active NOTCH2 ( intra cellular NOTCH2 ).Lanes 1-3 contain lysate 24 h ( 1 ), 48 h ( 2 ), and 72 h ( 3 ) post transfection.Lanes 4-6 are the corresponding control cells ( untransfected ) taken at similar timepoints.The band at about 110 kD represents active NOTCH2.This band is not seen in the control cell.The intracellular domain of NOTCH2 has a predicted band size of 110kD, corresponding to this band.Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milkand probed with NOTCH2 polyclonal antibody at a 1 : 400 dilution.ECL was used as visualization method.
Human 721_B; WB Suggested Anti-NOTCH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. There is BioGPS gene expression data showing that NOTCH2 is expressed in 721_B.; NOTCH2 antibody - middle region (ARP32723_P050) in Human 721_B cells using Western Blot
Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with NOTCH2 antibody AP22760PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Liver (formalin-fixed, paraffin-embedded) stained with NOTCH2 antibody AP22760PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with NOTCH2 antibody AP22760PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemical analysis of NOTCH2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of NOTCH2 expression in HeLa (A); MCF7 (B); rat liver (C); NIH3T3 (D) whole cell lysates.
Western blot analysis of NOTCH2 expression in HepG2 colchicine-treated (A); Raw264.7 colchicine-treated (B); rat liver (C) whole cell lysates.
Western blot analysis of extracts from 293 cells treated with TNF-a (20ng/ml, 30mins), using Notch 2 (cleaved-Asp1733) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Notch 2 (cleaved-Asp1733) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Jurkat cells, treated with etoposide (25uM, 24hours), using NOTCH2 (Cleaved-Ala1734) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Jurkat cells, treated with etoposide (25uM, 24hours), using NOTCH2 (Cleaved-Val1697) antibody.The lane on the right is treated with the synthesized peptide.
anti-NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
WB using anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein (arrowhead). WB analysis was performed for Notch 2 expression using human mesothelial SV40 cells lysate obtained fromtransfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected). Anti-Notch 2 was used at 1:400.
Anti-Notch 2 antibody was diluted 1:500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using NOTCH2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification: x200)
WB Suggested Anti-NOTCH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysateThere is BioGPS gene expression data showing that NOTCH2 is expressed in 721_B
Western blot analysis of NOTCH2 (Cleaved-Ala1734) expression in Jurkat cells, treated with etoposide
Western blot analysis of Notch 2 (Cleaved-Asp1733) expression in293 cell extract treated with TNF-alpha
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311717 | NOTCH2 antibody |
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| Rabbit polyclonal Notch 2 (Cleaved-Asp1733) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA314277 | NOTCH2 antibody |
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| Rabbit polyclonal NOTCH2 (Cleaved-Ala1734) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA314278 | NOTCH2 antibody |
||||||
| Rabbit polyclonal NOTCH2 (Cleaved-Val1697) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA319402 | NOTCH2 (N-term of NICD) antibody |
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| Rabbit polyclonal anti-NOTCH 2 antibody | |||||||
 |
Rabbit | IgG | Serum | Hu, Ms, Rt | E, P, WB |
0.2 ml /
€550.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA319403 | NOTCH2 (Intra) antibody |
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| Rabbit polyclonal anti-NOTCH 2 antibody | |||||||
 |
Rabbit | Serum | Can, Chimp, Hu, Ms, Rt | E, P, WB |
0.2 ml /
€550.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA322182 | NOTCH2 antibody |
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| Anti-NOTCH2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347941 | NOTCH2 antibody |
||||||
| Rabbit Polyclonal Anti-NOTCH2 (Cleaved-Ala1734) Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347961 | NOTCH2 antibody |
||||||
| Rabbit Polyclonal Anti-Notch 2 (Cleaved-Asp1733) Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP07611SU-N | NOTCH2 (2396-2409) antibody |
||||||
 |
Rabbit | Serum | Can, Hu | E, ICC/IF, P, WB |
50 µl /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP07864SU-N | NOTCH2 antibody |
||||||
 |
Rabbit | Supernatant | Hu | E, P |
50 µl /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| AP22760PU-N | NOTCH2 (2457-2471) antibody |
||||||
 |
Rabbit | Purified | Elephant, Hu, Mky | E, P |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| GT15124-100 | NOTCH2 antibody |
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 |
Goat | Aff - Purified | Rt | C, E, F, ICC/IF, WB |
0.1 mg /
€450.00
|
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| Neuromics Antibodies | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN215107LP | NOTCH2 - human gene knockout kit via CRISPR |
||
 |
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN215107RB | NOTCH2 - human gene knockout kit via CRISPR |
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|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN311125 | Notch2 - mouse gene knockout kit via CRISPR |
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|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN311125BN | Notch2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN311125LP | Notch2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN311125RB | Notch2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
