NOXA1 antibody
Principal name
NOXA1 antibody
Alternative names for NOXA1 antibody
P51NOX, NADPH oxidase activator, P67phox-like factor, p51-nox, NCF2-like protein, Antigen NY-CO-31
Ncbi ID
9606, NP_006638, NP_006638.1, NM_006647, NP_001157098.1, NM_172204, NM_001163626, NM_001100171
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP)
Background of NOXA1 antibody
NOXA1 (NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz (2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence (build 36.1). Banfi et al. (2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al. (2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX (CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.
General readings
Niu, X.L. et al., Circulation 121(4): 549-559 (2010)
Pacquelet, S. et al., J. Biol. Chem. 283(36): 24649-24658 (2008)
Honjo, T. et al., Endothelium 15(3): 137-141 (2008)
Kim, J.S. et al., J. Biol. Chem. 282(48): 34787-34800 (2007)
Valente, A.J. et al., Free Radic. Biol. Med. 43(3): 384-396 (2007)
Cheng, G. et al., J. Biol. Chem. 281(26): 17718-17726 (2006)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52915PU-N | NOXA1 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308357 | NOXA1 |
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| Recombinant protein of human NADPH oxidase activator 1 (NOXA1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416502 | NOXA1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207092L2V | Lenti ORF particles, Noxa1 (GFP-tagged) - Mouse NADPH oxidase activator 1 (Noxa1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,100.00
|
|
| OriGene Technologies, Inc. | ||
| MR207092L1V | Lenti ORF particles, Noxa1 (Myc-DDK-tagged) - Mouse NADPH oxidase activator 1 (Noxa1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€990.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR200990L2V | Lenti ORF particles, Noxa1 (GFP-tagged ORF) - Rat NADPH oxidase activator 1 (Noxa1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| RR200990L1V | Lenti ORF particles, Noxa1 (Myc-DDK-tagged ORF) - Rat NADPH oxidase activator 1 (Noxa1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€990.00
|
|
| OriGene Technologies, Inc. | ||