NR0B2 antibody
Principal name
NR0B2 antibody
Alternative names for NR0B2 antibody
SHP, Nuclear receptor subfamily 0 group B member 2,Orphan nuclear receptor SHP, Small heterodimer partner
Ncbi ID
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection))
Background of NR0B2 antibody
NR0B2 is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. It is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Park,W.I., (2008) Biochem. Biophys. Res. Commun. 369 (2), 437-443
NR0B2 antibody (Center) (Cat. #AP52928PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR0B2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of NR0B2 Antibody (Center)(Cat#AP52928PU-N) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
NR0B2 Antibody (Center)(PEI 1/100) (Cat. #AP52928PU-N) western blot analysis in HepG2 cell line lysates (35µg/lane).This demonstrates the NR0B2 antibody detected the NR0B2 protein (arrow).
Transfected 293T; WB Suggested Anti-NR0B2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; NR0B2 antibody - middle region (ARP36012_P050) in Transfected 293T cells using Western Blot
Transfected 293T; WB Suggested Anti-NR0B2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; NR0B2 antibody - C-terminal region (ARP36013_P050) in Transfected 293T cells using Western Blot
Transfected 293T; WB Suggested Anti-NR0B2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; NR0B2 antibody - N-terminal region (ARP45635_P050) in Transfected 293T cells using Western Blot
Human Liver; Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-NR0B2 antibody (ARP45636_P050); NR0B2 antibody - middle region (ARP45636_P050) in Human Liver cells using Immunohistochemistry
Transfected 293T; WB Suggested Anti-NR0B2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; NR0B2 antibody - middle region (ARP45636_P050) in Transfected 293T cells using Western Blot
Western Blot analysis of NR0B2 expression in transfected 293T cell line (H00008431-T01) by NR0B2 MaxPab polyclonal antibody.Lane 1: NR0B2 transfected lysate(28.27 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged NR0B2 is approximately 3ng/ml as a capture antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B2 (RC206422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR0B2.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B2 (RC206422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR0B2.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806319) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806319) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806319) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B2 (RC206422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR0B2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806321) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B2 (RC206422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR0B2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B2 (RC206422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR0B2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B2 (RC206422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR0B2.(1:2000)
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using NR0B2(TA308392) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using NR0B2(TA308392) antibody at 1:500 dilution.
WB Suggested Anti-NR0B2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T
WB Suggested Anti-NR0B2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T
Western blot analysis of extracts of various cell lines, using NR0B2 antibody.
Immunohistochemistry of paraffin-embedded rat lung using NR0B2 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded rat brain using NR0B2 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NR0B2 antibody at dilution of 1:100 (400x lens).
WB Suggested Anti-NR0B2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
WB Suggested Anti-NR0B2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0 ug/ml using anti-NR0B2 antibody
Anti-NR0B2 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332442 | NR0B2 antibody |
||||||
| Rabbit anti-NR0B2 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
||||||
| TA806319 | NR0B2 antibody |
||||||
| NR0B2 mouse monoclonal antibody, clone OTI5F10 (formerly 5F10) | |||||||
 |
Mouse | IgG2a | OTI5F10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA806321 | NR0B2 antibody |
||||||
| NR0B2 mouse monoclonal antibody, clone OTI7B5 (formerly 7B5) | |||||||
 |
Mouse | IgG2a | OTI7B5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +20 additional images |
Highly validated antibody!
|
||||||
| TA806345 | NR0B2 antibody |
||||||
| NR0B2 mouse monoclonal antibody, clone OTI8E5 (formerly 8E5) | |||||||
 |
Mouse | IgG1 | OTI8E5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
||||||
| TA308392 | NR0B2 antibody |
||||||
| Rabbit Polyclonal antibody to NR0B2 (nuclear receptor subfamily 0, group B, member 2) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA340514 | NR0B2 antibody |
||||||
| Rabbit Polyclonal Anti-NR0B2 Antibody (Ligand-binding Domain) | |||||||
 |
Rabbit | Aff - Purified | Hu | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA806268 | NR0B2 antibody |
||||||
| NR0B2 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) | |||||||
 |
Mouse | IgG1 | OTI1A2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA806318 | NR0B2 antibody |
||||||
| NR0B2 mouse monoclonal antibody, clone OTI6D8 (formerly 6D8) | |||||||
 |
Mouse | IgG2b | OTI6D8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA806398 | NR0B2 antibody |
||||||
| NR0B2 mouse monoclonal antibody, clone OTI2B6 (formerly 2B6) | |||||||
 |
Mouse | IgG2a | OTI2B6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| AP52928PU-N | NR0B2 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760144 | NR0B2 (full length, N-term HIS tag) |
||||
| Recombinant protein of human nuclear receptor subfamily 0, group B, member 2 (NR0B2), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402893 | NR0B2 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211116L2V | Lenti ORF particles, Nr0b2 (GFP-tagged ORF) - Rat nuclear receptor subfamily 0, group B, member 2 (Nr0b2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR211116L1V | Lenti ORF particles, Nr0b2 (Myc-DDK-tagged ORF) - Rat nuclear receptor subfamily 0, group B, member 2 (Nr0b2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
