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NR1D2 antibody

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Principal name

NR1D2 antibody

Alternative names for NR1D2 antibody

Rev-erb-beta, EAR-1R, Orphan nuclear hormone receptor BD73, Nuclear receptor subfamily 1 group D member 2

SwissProt ID

Q14995 (Human), Q60674 (Mouse), Q63504 (Rat)

Gene ID

9975 (NR1D2), 353187 (Nr1d2)

Ncbi ID

9606, NP_005117, NP_005117.3, NP_035714.3, NM_005126, NM_001145425

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bt (Bat), Eq (Equine), Mky (Monkey), Op (Opossum), Prim (Primate), Rb (Rabbit), Bear, Gorilla, GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of NR1D2 antibody

Rev-erbA beta, a NR1 Thyroid Hormone-Like Receptor, has been shown to affect cell proliferation, organ physiology, and myogenesis. Rev-erbA beta has been shown to be a constitutive repressor and binds as a monomer or homodimer to target DNA, such as the n-myc gene. Rev-erbA beta has also been shown to repress the human apoCIII gene, therefore underscoring a physiological role in the regulation of genes involved in triglyceride metabolism and the pathogenesis of atherosclerosis. Two isoforms of Rev-erbA beta have been isolated in rats (Rev-erbA beta 1 and Rev-erbA beta 2). Rev-erbA beta 2 is longer than Rev-erbA beta 1 by 195 amino acids.
Rev-erbA beta expression has been documented in animal brain, skeletal muscle, embryo, and spleen, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous brain, colon, eye, kidney, lung, prostate, skin, and uterus, and normal brain, colon, embryo, heart, kidney, liver, lung, nerve, pineal, placenta, skeletal muscle, testis, tonsil, and uterus.

General readings

Sauve,F., et al., (2001) Mol. Cell. Biol. 21 (1), 343-353

H00009975-M01 - NR1D2
Immunofluorescence of monoclonal antibody to NR1D2 on HeLa cell. [antibody concentration 10 ug/ml]
H00009975-M01 - NR1D2
Western blot analysis of NR1D2 over-expressed 293 cell line, cotransfected with NR1D2 Validated Chimera RNAi ( Cat # H00009975-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D2 monoclonal antibody (M01), clone 1G2 (Cat # H00009975-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00009975-M01 - NR1D2
Western Blot analysis of NR1D2 expression in transfected 293T cell line by NR1D2 monoclonal antibody (M01), clone 1G2.Lane 1: NR1D2 transfected lysate(64.7 KDa).Lane 2: Non-transfected lysate.
H00009975-M01 - NR1D2
NR1D2 monoclonal antibody (M01), clone 1G2 Western Blot analysis of NR1D2 expression in Hela ( Cat # L013V1 ).
ARP38568_T100 - NR1D2
Human Jurkat; WB Suggested Anti-NR1D2 Antibody Titration: 0.5ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; NR1D2 antibody - C-terminal region (ARP38568_T100) in Human Jurkat cells using Western Blot
H00009975-B01 - NR1D2
Western Blot analysis of NR1D2 expression in transfected 293T cell line (H00009975-T01) by NR1D2 MaxPab polyclonal antibody.Lane1:NR1D2 transfected lysate(63.69 KDa).Lane2:Non-transfected lysate.
H00009975-D01P - NR1D2
NR1D2 MaxPab rabbit polyclonal antibody. Western Blot analysis of NR1D2 expression in mouse liver.
H00009975-D01P - NR1D2
Western Blot analysis of NR1D2 expression in transfected 293T cell line (H00009975-T02) by NR1D2 MaxPab polyclonal antibody.Lane 1: NR1D2 transfected lysate(64.70 KDa).Lane 2: Non-transfected lysate.
H00009975-D01P - NR1D2
NR1D2 MaxPab rabbit polyclonal antibody. Western Blot analysis of NR1D2 expression in HeLa.
ARP38567_P050 - NR1D2
Human Jurkat; WB Suggested Anti-NR1D2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; NR1D2 antibody - N-terminal region (ARP38567_P050) in Human Jurkat cells using Western Blot
ARP33671_P050 - NR1D2
Human THP-1; WB Suggested Anti-NR1D2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; NR1D2 antibody - middle region (ARP33671_P050) in Human THP-1 cells using Western Blot
TA806365 - NR1D2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2.
TA806366 - NR1D2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2.
TA806369 - NR1D2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2.
TA806588 - NR1D2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2.
TP306338 - NR1D2
TA317086 - NR1D2
Anti-NR1D2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA317086 - NR1D2
Anti-NR1D2 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA317087 - NR1D2
Anti-NR1D2 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA317087 - NR1D2
Anti-NR1D2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA317088 - NR1D2
Anti-NR1D2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA317089 - NR1D2
Anti-NR1D2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA317089 - NR1D2
Anti-NR1D2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA324572 - NR1D2
NR1D2 Antibody (Center) (Cat. #TA324572) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NR1D2 antibody detected the NR1D2 protein (arrow).
TA324572 - NR1D2
NR1D2 Antibody (Center) (TA324572)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR1D2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
TA334217 - NR1D2
WB Suggested Anti-NR1D2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: THP-1 cell lysate
TA343507 - NR1D2
WB Suggested Anti-NR1D2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
TA343508 - NR1D2
WB Suggested Anti-NR1D2 Antibody Titration: 0.5 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NR1D2 (ligand bindig dom.) antibody
  NR1D2 Rabbit Polyclonal (Ligand-binding Domain) Antibody  
NR1D2 Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hu, Mky, Ms, Op, Platypus, Prim, Rb, Rt P
50 µg / €425.00
  OriGene Technologies, Inc.

NR1D2 (C-term) antibody
  NR1D2 Rabbit Polyclonal (Internal) Antibody  
NR1D2 Rabbit IgG Aff - Purified Hu, Ms, Rt P
50 µg / €425.00
  OriGene Technologies, Inc.

NR1D2 antibody
  NR1D2 Rabbit Polyclonal (Ligand-binding Domain) Antibody  
Anti-NR1D2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Gorilla, Hu, Mky
50 µg / €425.00
  OriGene Technologies, Inc.

NR1D2 antibody
  NR1D2 Rabbit Polyclonal (N-Terminus) Antibody  
Anti-NR1D2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Can, Hu, Mky
50 µg / €425.00
  OriGene Technologies, Inc.
+1 additional image

NR1D2 (Center) antibody
  Rabbit polyclonal NR1D2 Antibody (Center)  
NR1D2 Antibody (Center) (Cat. #TA324572) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NR1D2 antibody detected the NR1D2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

NR1D2 antibody
  NR1D2 mouse monoclonal antibody, clone OTI5E10 (formerly 5E10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2. Mouse IgG2a OTI5E10 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

NR1D2 antibody
  NR1D2 mouse monoclonal antibody, clone OTI4G1 (formerly 4G1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2. Mouse IgG1 OTI4G1 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

NR1D2 antibody
  NR1D2 mouse monoclonal antibody, clone OTI5C6 (formerly 5C6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2. Mouse IgG1 OTI5C6 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

NR1D2 antibody
  NR1D2 mouse monoclonal antibody, clone OTI3D4 (formerly 3D4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2. Mouse IgG2a OTI3D4 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NR1D2 (transcript variant 1)
  Recombinant protein of human nuclear receptor subfamily 1, group D, member 2 (NR1D2), transcript variant 1  
NR1D2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

10 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (9)
  2. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (5)
  2. Monoclonal (4)
Target
  1. NR1D2 (10)
Property
  1. C-term (1)
  2. Center (1)
  3. ligand bindig dom. (1)
Reactivity
  1. Human (Hu) (9)
  2. Monkey (Mky) (3)
  3. Canine (Can) (2)
  4. Mouse (Ms) (2)
  5. Rat (Rt) (2)
  6. Bear (1)
  7. Bovine (Bov) (1)
  8. Bat (Bt) (1)
  9. Chicken (Chk) (1)
  10. Elephant (1)
  11. Equine (Eq) (1)
  12. Gorilla (1)
  13. Opossum (Op) (1)
  14. Platypus (1)
  15. Primate (Prim) (1)
  16. Rabbit (Rb) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (2)
Presentation
  1. Purified (6)
  2. Aff - Purified (3)
Host
  1. Rabbit (5)
  2. Mouse (4)
Images
  1. 1 image (8)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. IHC (3)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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