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NR2F6 antibody

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Principal name

NR2F6 antibody

Alternative names for NR2F6 antibody

EAR2, ERBAL2, Nuclear receptor subfamily 2 group F member 6,Orphan nuclear receptor EAR-2, V-erbA-related protein EAR-2

SwissProt ID

O09017 (Rat), P10588 (Human), P43136 (Mouse)

Gene ID

2063 (NR2F6), 245980 (Nr2f6)

Ncbi ID

NP_005225.2, NP_005225, 9606, NM_139113

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Ze (Zebrafish), African clawed frog, Bov (Bovine), Por (Porcine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Background of NR2F6 antibody

Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).

General readings

1. Weatherford ET  et al. Regulation of renin expression by the orphan nuclear receptors Nr2f2 and Nr2f6. Am J Physiol Renal Physiol 302:F1025-33 (2012). WB .PubMed: 22278040
2. Modica S  et al. The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors. Gastroenterology 138:636-48, 648.e1-12 (2010). IHC-P ; Human .PubMed: 19818784

AP06664PU-N - NR2F6
Immunohistochemistry (IHC) analyzes of EAR2 antibody (Cat.-No.: AP06664PU-N) in paraffin-embedded human cervix carcinoma tissue.
AP06664PU-N - NR2F6
Western blot (WB) analysis of EAR2 antibody (Cat.-No.: AP06664PU-N) in extracts from HeLa cells.
AP52933PU-N - NR2F6
NR2F6 Antibody (N-term) (Cat. #AP52933PU-N) western blot analysis in MCF-7 cell line lysates (35µg/lane).This demonstrates the NR2F6 antibody detected the NR2F6 protein (arrow).
ARP32256_P050 - NR2F6
Human Jurkat; WB Suggested Anti-NR2F6 Antibody. . Titration: 0.2 ug/ml. . Positive Control: Jurkat Whole Cell; NR2F6 antibody - N-terminal region (ARP32256_P050) in Human Jurkat cells using Western Blot
ARP32256_T100 - NR2F6
Human HepG2; WB Suggested Anti-NR2F6 Antibody. . Titration: 5 ug/ml. . Positive Control: HepG2 Whole Cell. NR2F6 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; NR2F6 antibody - N-terminal region (ARP32256_T100) in Human HepG2 cells using Western Blot
ARP32995_T100 - NR2F6
Human HepG2; WB Suggested Anti-NR2F6 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate. NR2F6 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; NR2F6 antibody - middle region (ARP32995_T100) in Human HepG2 cells using Western Blot
ARP45627_P050 - NR2F6
Human HepG2; WB Suggested Anti-NR2F6 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. NR2F6 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; NR2F6 antibody - N-terminal region (ARP45627_P050) in Human HepG2 cells using Western Blot
C10336-1 - NR2F6
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using NR2F6 Antibody. The picture on the right is treated with the synthesized peptide.
C10336-1 - NR2F6
Immunofluorescence analysis of HepG2 cells, using NR2F6 Antibody. The picture on the right is treated with the synthesized peptide.
C10336-1 - NR2F6
Western blot analysis of extracts from HeLa/HuvEc/293 cells, using NR2F6 Antibody. The lane on the right is treated with the synthesized peptide.
CPA1395-100ul - NR2F6
Immunohistochemical analysis of NR2F6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1395-100ul - NR2F6
Immunofluorescent analysis of NR2F6 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA1395-100ul - NR2F6
Western blot analysis of NR2F6 expression in HepG2 (A); rat liver (B) whole cell lysates.
TA806769 - NR2F6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2F6 (RC201336, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR2F6.
TA806787 - NR2F6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2F6 (RC201336, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR2F6.
TP301336 - NR2F6
TA311673 - NR2F6
Western blot analysis of extracts from HeLa cells, 293 cells and HUVEC cells, using NR2F6 antibody.The lane on the right is treated with the synthesized peptide.
TA311673 - NR2F6
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue using NR2F6 antibody.The picture on the right is treated with the synthesized peptide.
TA311673 - NR2F6
Immunofluorescence analysis of HepG2 cells, using NR2F6 antibody.The picture on the right is treated with the synthesized peptide.
TA330216 - NR2F6
WB Suggested Anti-NR2F6 Antibody; Titration: 0.2 ug/ml; Positive Control: Jurkat Whole Cell
TA330217 - NR2F6
WB Suggested Anti-NR2F6 Antibody; Titration: 5 ug/ml; Positive Control: HepG2 Whole Cell NR2F6 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
TA332066 - NR2F6
WB Suggested Anti-NR2F6 Antibody Titration: 5.0ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysateNR2F6 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
TA340518 - NR2F6
Anti-EAR2 / NR2F6 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA340518 - NR2F6
Anti-NR2F6 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA340518 - NR2F6
Anti-EAR2 / NR2F6 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA340519 - NR2F6
Anti-EAR2 / NR2F6 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA340519 - NR2F6
Anti-NR2F6 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA338164 - NR2F6
WB Suggested Anti-NR2F6 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate. NR2F6 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
TA338164 - NR2F6
Human liver

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NR2F6 antibody
  Rabbit polyclonal anti-NR2F6 antibody  
Western blot analysis of extracts from HeLa cells, 293 cells and HUVEC cells, using NR2F6 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

NR2F6 (Ligand-Binding Dom.) antibody
  Rabbit Polyclonal Anti-NR2F6 Antibody (Ligand-binding Domain)  
NR2F6 Rabbit Aff - Purified Hu P
50 µg / €425.00
  OriGene Technologies, Inc.

NR2F6 (N-term) antibody
  Rabbit Polyclonal Anti-NR2F6 Antibody (N-Terminus)  
NR2F6 Rabbit Aff - Purified Hu P
50 µg / €425.00
  OriGene Technologies, Inc.

NR2F6 antibody
Immunohistochemistry (IHC) analyzes of EAR2 antibody (Cat.-No.: AP06664PU-N) in paraffin-embedded human cervix carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

NR2F6 (N-term) antibody
NR2F6 Antibody (N-term) (Cat. #AP52933PU-N) western blot analysis in MCF-7 cell line lysates (35µg/lane).This demonstrates the NR2F6 antibody detected the NR2F6 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

5 Item(s)

per page

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  1. Primary Antibody (5)
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  1. NR2F6 (5)
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  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
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  2. Western blot / Immunoblot (WB) (3)
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  2. 3+ images (1)
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