NR4A3 antibody
Principal name
NR4A3 antibody
Alternative names for NR4A3 antibody
CHN, CSMF, MINOR, NOR1, TEC, Nuclear hormone receptor NOR-1, Neuron-derived orphan receptor 1, Mitogen-induced nuclear orphan receptor
Ncbi ID
9606, AAB02581, NP_008912.2, NM_015743, NP_775292.1, NP_775292, NM_031628, NM_006981, NM_173198
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of NR4A3 antibody
NOR-1, a NR4 nerve growth factor-like receptor, has been shown to affect neuronal development, T-cell apoptosis, and cancer development. NOR-1 binds to NBRE (NGFI-B response element) and regulates the pro-opiomelanocortin (POMC) gene. NOR-1 is induced rapidly but transiently by a variety of stimuli. Downregulation of NOR-1 and other NGFI-B family members is correlated with drug addiction-prone behavior of Lewis rats. Three human protein isoforms have been documented: NOR-1, NOR-1beta, and NOR-1 3' variant.
NOR-1 expression has been documented in human brain, heart, placenta, kidney, skeletal muscle, fetal brain and fetal lung. ESTs have been isolated from human tissue libraries, including cancerous head/neck and skin, and normal prostate, skin, liver, testis, skeletal muscle, blood and B-cell/lung/testis. The symbol NOR-1 is also used to describe a nitric oxide-donor reagent. Tec is also the name of a kinase on chromosome 4.
Ligand: 6-mercaptopurine Abayratna
Immunofluorescence of monoclonal antibody to NR4A3 on HeLa cell. [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged NR4A3 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to NR4A3 on HeLa cell. [antibody concentration 10 ug/ml]
NR4A3 monoclonal antibody (M02), clone 1E9 Western Blot analysis of NR4A3 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged NR4A3 is approximately 0.3ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-NR4A3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; NR4A3 antibody - middle region (ARP45646_P050) in Human HepG2 cells using Western Blot
Mouse Brain; WB Suggested Anti-Nr4a3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Brain; Nr4a3 antibody - C-terminal region (ARP37214_P050) in Mouse Brain cells using Western Blot
NR4A3 Antibody (C-term) (Cat. #AP52934PU-N) western blot analysis in HepG2 cell line lysates (35µg/lane).This demonstrates the NR4A3 antibody detected the NR4A3 protein (arrow).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804854)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804854)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804854)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804854)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804854)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3 (RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804855)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804855)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804855)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804855)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3 (RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804872)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804872)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804872)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804872)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3 (RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3 (RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3 (RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA804893) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NR4A3 Mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA804893) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA804893) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA804893) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA804893) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA804893) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA804893) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3 (RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3 (RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3 (RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3.
Western blot analysis of extracts from COLO cells, using NR4A3 antibody.The lane on the right is treated with the synthesized peptide.
Figure from citation: Co-IP assay was performed to show the interaction between NOR1 (also known as NR4A3) and PARP-1 after immunoprecipitation of cardiomyocyte nuclear extracts with anti-NR4A3 antibody. Dilution: 1:300 View Citation
Anti-Nor-1 / NR4A3 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Nor-1 / NR4A3 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR4A3 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Nor-1 / NR4A3 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Nor-1 / NR4A3 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR4A3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR4A3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Nor-1 / NR4A3 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR4A3 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
WB Suggested Anti-Nr4a3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Brain
Western blot analysis on COLO205 cell lysate using NR4A3 Antibody
- Quick-Filters:
- Highly Validated (5)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA340876 | NR4A3 antibody |
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| Rabbit Polyclonal Anti-NR4A3 Antibody (Internal) | |||||||
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Rabbit | Purified | Bt, Chk, Hst, Hu, Mky, Ms, Rt |
50 µg /
€425.00
|
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA804854 | NR4A3 antibody |
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| NR4A3 mouse monoclonal antibody, clone OTI2B11 (formerly 2B11) | |||||||
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Mouse | IgG1 | OTI2B11 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA804855 | NR4A3 antibody |
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| NR4A3 mouse monoclonal antibody, clone OTI2A6 (formerly 2A6) | |||||||
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Mouse | IgG1 | OTI2A6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA804872 | NR4A3 antibody |
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| NR4A3 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1) | |||||||
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Mouse | IgG1 | OTI2E1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA804893 | NR4A3 antibody |
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| NR4A3 mouse monoclonal antibody, clone OTI5C2 (formerly 5C2) | |||||||
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Mouse | IgG1 | OTI5C2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA314712 | NR4A3 antibody |
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| Rabbit polyclonal anti-NR4A3 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA340875 | NR4A3 (modulation domain) antibody |
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| Rabbit Polyclonal Anti-NR4A3 Antibody (Modulating Domain) | |||||||
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Rabbit | Aff - Purified | Hu, Mky, Prim | P |
50 µg /
€425.00
|
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| OriGene Technologies, Inc. | |||||||
| TA340877 | NR4A3 antibody |
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| Rabbit Polyclonal Anti-NR4A3 Antibody (C-Terminus) | |||||||
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Rabbit | Purified | Bov, Hst, Hu, Mky, Ms, Por, Rb, Rt |
50 µg /
€425.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA347578 | NR4A3 antibody |
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| Rabbit Polyclonal Anti-NR4A3 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA804891 | NR4A3 antibody |
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| NR4A3 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1) | |||||||
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Mouse | IgG1 | OTI1C1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804892 | NR4A3 antibody |
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| NR4A3 mouse monoclonal antibody, clone OTI3H1 (formerly 3H1) | |||||||
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Mouse | IgG1 | OTI3H1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA804894 | NR4A3 antibody |
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| NR4A3 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5) | |||||||
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Mouse | IgG1 | OTI1C5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA804895 | NR4A3 antibody |
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| NR4A3 mouse monoclonal antibody, clone OTI4D1 (formerly 4D1) | |||||||
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Mouse | IgG1 | OTI4D1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| AP52934PU-N | NR4A3 (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP323629 | NR4A3 (transcript variant 3) |
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| Recombinant protein of human nuclear receptor subfamily 4, group A, member 3 (NR4A3), transcript variant 3 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR200342L2V | Lenti ORF particles, Nr4a3 (GFP-tagged ORF) - Rat nuclear receptor subfamily 4, group A, member 3 (Nr4a3), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,370.00
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| OriGene Technologies, Inc. | ||
| RR200342L1V | Lenti ORF particles, Nr4a3 (Myc-DDK-tagged ORF) - Rat nuclear receptor subfamily 4, group A, member 3 (Nr4a3), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,230.00
|
|
| OriGene Technologies, Inc. | ||
