Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » NRAS antibody

NRAS antibody

(reset)

Principal name

NRAS antibody

Alternative names for NRAS antibody

GTPase NRas, N-Ras, HRAS1, ALPS4, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog

SwissProt ID

P01111 (Human), P08556 (Mouse), P12825 (Cavpo), P79737 (Danre), Q04970 (Rat), Q2MJK3 (Pig), Q5F352 (Chick), Q91806 (Xenla)

Gene ID

4893 (NRAS), 24605 (Nras)

Ncbi ID

9606, NP_002515.1, NP_203524, NP_002515, NM_080766, NM_002524

Available reactivities

Hu (Human), Rt (Rat), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Ms (Mouse), Op (Opossum), Por (Porcine), Prim (Primate), Bear, Dros (Drosophila), African clawed frog, Chk (Chicken)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of NRAS antibody

Kit Component:
- KN202681G1, NRAS gRNA vector 1 in pCas-Guide vector
- KN202681G2, NRAS gRNA vector 2 in pCas-Guide vector
- KN202681D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Thomas NE, Edmiston SN, Alexander A, Millikan RC, Groben PA, Hao H, Tolbert D, Berwick M, Busam K, Begg CB, Mattingly D, Ollila DW, Tse CK, Hummer A, Lee-Taylor J, Conway K. Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma. Cancer Epidemiol Biomarkers Prev. 2007 May;16(5):991-7.
PMID: 17507627

H00004893-M01 - NRAS
Detection limit for recombinant GST tagged NRAS is approximately 0.1ng/ml as a capture antibody.
PAB3461 - NRAS
Western blot analysis of NRAS ( arrow ) using rabbit NRAS polyclonal antibody ( Cat # PAB3461 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the NRAS gene ( Lane 2 ) ( Origene Technologies ) .
PAB3461 - NRAS
Western blot analysis of NRAS polyclonal antibody ( Cat # PAB3461 ) in CEM cell line lysates ( 35 µg/lane ) . NRAS ( arrow ) was detected using the purified polyclonal antibody.
PAB7450 - NRAS
NRAS polyclonal antibody ( Cat # PAB7450 ) ( 2.5 µg/ml ) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
PAB7450 - NRAS
293 overexpressing NRAS and probed with NRAS polyclonal antibody ( Cat # PAB7450 ) ( mock transfection in first lane), tested by Origene.
PAB7450 - NRAS
NRAS polyclonal antibody ( Cat # PAB7450 ) ( 0.01 µg/ml ) staining of A-431 lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
AP08843PU-N - NRAS
IHC Image: Small intestine
AP08843PU-N - NRAS
Antibody (0.01 µg/ml) staining of A431 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ARP55408_P050 - NRAS
candida albicans Ras1P; . Lanes: Lane 1: 250ng candida albicans Ras1P protein. Primary Antibody Dilution: 1:5000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:10,000. Gene Name: KRAS. Submitted by: Dr. Alexandra Oliveira, Instituto de Biologia Molecular e Celular.; KRAS antibody - N-terminal region (ARP55408_P050) in candida albicans Ras1P cells using Western Blot
ARP55408_P050 - NRAS
Human Heart; WB Suggested Anti-KRAS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; KRAS antibody - N-terminal region (ARP55408_P050) in Human Heart cells using Western Blot
AR09391PU-L - NRAS
AR09391PU-L - NRAS
Recombinant human NRAS, 1-186 aa: 15% SDS-PAGE (3 µg)
AM09391PU-N - RAS
ICC/IF analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human RAS antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green).
AM09391PU-N - RAS
Flow cytometry analysis of RAS in HeLa cell line, staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
AM09391PU-N - RAS
Western blot analysis: The extracts of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The cross-reacting of anti-human RAS (AT2G9) was analyzed by Western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS.
AM32436PU-N - NRAS
SK-MEL-2 cells stained with anti-N-Ras (Q61R) antibody
AM32436PU-N - NRAS
Western Blot analysis of SK-MEL-2 cell lysate with N-Ras (Q61R) antibody
CPA1549-100ul - NRAS/HRAS/KRAS
Western blot analysis of NRAS/HRAS/KRAS expression in HEK293T (A); HeLa (B); mouse brain (C); rat heart (D) whole cell lysates.
TA505835 - NRAS
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505835)
TA505835 - NRAS
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRAS (RC202681, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRAS.
TA590133 - NRAS
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NRAS Rabbit Polyclonal antibody. (TA590133)
TA590133 - NRAS
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRAS (RC202681, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRAS.
TA590133 - NRAS
ELISA: NRAS Antibody
TP302681 - NRAS
TP701004 - NRAS
TA302554 - NRAS
TA302554 (0.01µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA324982 - NRAS
Western blot analysis of anti-NRAS Antibody (N-term)(Cat.#TA324982) in CEM cell line lysates (35ug/lane). NRAS(arrow) was detected using the purified Pab.
TA324982 - NRAS
Western blot analysis of NRAS (arrow) using rabbit polyclonal NRAS Antibody (N-term) (Cat.#TA324982). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NRAS gene (Lane 2) (Origene Technologies).
TA324982 - NRAS
Confocal immunofluorescent analysis of NRAS Antibody (N-term)(Cat#TA324982) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
TA324982 - NRAS
NRAS Antibody (N-term) (Cat. #TA324982) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA324983 - NRAS
NRAS Antibody (C-term) (Cat. #TA324983) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow).
TA324983 - NRAS
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with NRAS antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TA324983 - NRAS
NRAS Antibody (C-term) (Cat. #TA324983) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA326638 - NRAS
Western blot analysis of N-RAS in 293 cell lysate with N-RAS antibody at (A) 1 and (B) 2 ug/mL.
TA328115 - NRAS
NRAS Antibody (N-term) (Cat. #TA328115) western blot analysis in A431 and rat C6 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow).
TA347465 - NRAS
Western blot analysis on HeLa cell lysate using RASH/RASK/RASN Antibody
TA351463 - NRAS
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue, Jurkat cells, Primary antibody: (NRAS Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
TA351463 - NRAS
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (NRAS Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NRAS antibody
  Goat Polyclonal Antibody against NRAS  
TA302554 (0.01µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

NRAS (N-term) antibody
  Rabbit polyclonal NRAS Antibody (N-term)  
Western blot analysis of anti-NRAS Antibody (N-term)(Cat.#TA324982) in CEM cell line lysates (35ug/lane). NRAS(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, ICC/IF, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+3 additional images

NRAS (C-term) antibody
  Rabbit polyclonal NRAS Antibody (C-term)  
NRAS Antibody (C-term) (Cat. #TA324983) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow). Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

NRAS antibody
  Rabbit Polyclonal Anti-Ras Antibody  
NRAS Rabbit Purified Bov, Hu, Ms, Rt WB
0.1 mg / €365.00
  OriGene Technologies, Inc.

NRAS antibody
  Rabbit Polyclonal N-RAS Antibody  
Western blot analysis of N-RAS in 293 cell lysate with N-RAS antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €415.00
  OriGene Technologies, Inc.

NRAS antibody
  Mouse monoclonal anti-NRAS antibody (N-term)  
NRAS Antibody (N-term) (Cat. #TA328115) western blot analysis in A431 and rat C6 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow). Mouse IgG1 822CT17.5.1 Purified Chk, Hu, Ms, Por, Rt WB
0.4 ml / €415.00
  OriGene Technologies, Inc.

NRAS antibody
  Rabbit Polyclonal Anti-RASH/RASK/RASN Antibody  
Western blot analysis on HeLa cell lysate using RASH/RASK/RASN Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

NRAS antibody
  Rabbit Polyclonal Anti-NRAS Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue, Jurkat cells, Primary antibody: (NRAS Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

NRAS antibody
  NRAS mouse monoclonal antibody, clone OTI5G7 (formerly 5G7)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505835) Mouse IgG1 OTI5G7 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

NRAS antibody
  Rabbit Polyclonal NRAS Antibody  
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NRAS Rabbit Polyclonal antibody. (TA590133) Rabbit IgG Purified Hu E, P, WB
50 µg / €409.00
  OriGene Technologies, Inc.
+2 additional images

NRAS (167-179) antibody
IHC Image: Small intestine Goat IgG Aff - Purified Bov, Bt, Can, Elephant, Eq, Hu, Ms, Por E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+1 additional image

RAS (1-186) antibody
ICC/IF analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human RAS antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG2a AT2G9 Purified Hu E, F, ICC/IF, WB
0.1 ml / €330.00
  OriGene Technologies GmbH
+2 additional images

RAS (1-186) antibody
ICC/IF analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human RAS antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG2a AT2G9 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  OriGene Technologies GmbH
+2 additional images

CRISPR

Catalog No. Species Donor Vector  

NRAS - human gene knockout kit via CRISPR
NRAS - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NRAS - human gene knockout kit via CRISPR
NRAS - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NRAS - human gene knockout kit via CRISPR
NRAS - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NRAS
  Recombinant protein of human neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS)  
NRAS Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

NRAS
  Purified recombinant protein of Homo sapiens neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS), mutant (Q61K), expressed in HEK293 cells, 20ug  
NRAS Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nras (GFP-tagged ORF) - Rat neuroblastoma ras oncogene (Nras), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nras (GFP-tagged ORF) - Rat neuroblastoma ras oncogene (Nras), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

19 Item(s)

per page
19 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (3)
  2. Primary Antibody (13)
  3. Protein | Growth Factor (2)
  4. Rat Lenti ORF Particle (1)
Clonality
  1. Polyclonal (9)
  2. Monoclonal (4)
Target
  1. NRAS (13)
  2. RAS (2)
Property
  1. 1-186 (2)
  2. 167-179 (1)
  3. C-term (1)
  4. N-term (1)
Reactivity
  1. Human (Hu) (13)
  2. Mouse (Ms) (7)
  3. Rat (Rt) (6)
  4. Bovine (Bov) (2)
  5. Canine (Can) (2)
  6. Porcine (Por) (2)
  7. Bat (Bt) (1)
  8. Chicken (Chk) (1)
  9. Elephant (1)
  10. Equine (Eq) (1)
Applications
  1. Western blot / Immunoblot (WB) (13)
  2. Paraffin Sections (P) (6)
  3. Enzyme Immunoassay (E) (5)
  4. Flow cytometry (F) (3)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
Presentation
  1. Purified (11)
  2. Aff - Purified (2)
Host
  1. Rabbit (7)
  2. Mouse (4)
  3. Goat (2)
Images
  1. 1 image (14)
  2. 3+ images (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (12)
  2. Paraffin Sections (P) (3)
  3. Flow cytometry (F) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. IHC (2)
  6. Enzyme Immunoassay (E) (1)
  7. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (2)
Source
  1. HEK293 cells (2)
Species (CRISPR)
  1. Human (3)
Donor Vector
  1. Luciferase-Puro (1)
  2. RFP-BSD (1)
  3. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (19)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn