NRF1 antibody
Principal name
NRF1 antibody
Alternative names for NRF1 antibody
NRF-1, Alpha-pal, Nuclear respiratory factor 1, Alpha palindromic-binding protein
Gene ID
4899 (NRF1)
Ncbi ID
9606, NP_001035199.1, NP_005002, NP_001035199, 10090, NM_001040110, NM_005011
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of NRF1 antibody
NRF1 is a phosphorylated nuclear protein with a bZIP domain. This protein homodimerizes and functions as a transcription factor that activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial D transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial D transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alterte transcriptiol splice variants, which encode the same protein, have been characterized. Additiol variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for 'nuclear factor (erythroid-derived 2)-like 1' which has an official symbol of NFE2L1.
General readings
Chang,W.T., (2005) Biochem. Biophys. Res. Commun. 334 (1), 199-206
Immunoperoxidase of monoclonal antibody to NRF1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Western blot analysis of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi ( Cat # H00004899-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NRF1 monoclonal antibody (M01), clone 2F9 (Cat # H00004899-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate(55.7 KDa).Lane 2: Non-transfected lysate.
NRF1 monoclonal antibody (M01), clone 2F9. Western Blot analysis of NRF1 expression in human Skeletal muscle.
Detection limit for recombinant GST tagged NRF1 is approximately 0.03ng/ml as a capture antibody.
NRF1 polyclonal antibody (A01), Lot # ABNOVA060630QCS1 Western Blot analysis of NRF1 expression in Hela NE ( Cat # L013V3 ).
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Testis.
NRF1 MaxPab rabbit polyclonal antibody. Western Blot analysis of NRF1 expression in mouse kidney.
Western Blot analysis of NRF1 expression in transfected 293T cell line (H00004899-T01) by NRF1 MaxPab polyclonal antibody.Lane 1: NRF1 transfected lysate(55.70 KDa).Lane 2: Non-transfected lysate.
Human Lung; NRF1 antibody - C-terminal region (ARP38543_T100) in Human Lung cells using Immunohistochemistry
Transfected 293T; WB Suggested Anti-NRF1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; NRF1 antibody - C-terminal region (ARP38543_T100) in Transfected 293T cells using Western Blot
Immunohistochemistry of Nrf1 polyclonal antibody ( Cat # PAB10295 ) was used at a 5 µg/ml to detect NRF1 in a variety of tissues.This image shows Nrf1 staining of human testis.The antibody shows nuclear staining in lymphocytes.Tissue was formalin-fixed andparaffin embedded.Personal Communication, Tina Roush,Life Span Biosciences, Seattle, WA.
Western blot using Nrf1 polyclonal antibody ( Cat # PAB10295 ) shows detection of a 67 kDa band corresponding to human Nrf1 in a ( lane 1 ) HeLa nuclear extract and ( lane 2 ) whole cell lysate ( molecular weight marker not shown ).Approximately 10 µg of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose.The blot was incubated with a 1 : 500 dilution of the antibody at room temperature for 1 h followed by detection using IRDyeTM700 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 2,500.IRDyeTM700 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is atrademark of LI-COR, Inc.
Human Prostate; NRF1 Antibody - middle region (ARP34523_P050) in Human Prostate cells using Immunohistochemistry
Human 293T; Human 293T. There is BioGPS gene expression data showing that NRF1 is expressed in HEK293T.; NRF1 Antibody - middle region (ARP34523_P050) in Human 293T cells using Western Blot
Human Hela; Human Hela. There is BioGPS gene expression data showing that NRF1 is expressed in HeLa.; NRF1 Antibody - middle region (ARP34523_P050) in Human Hela cells using Western Blot
Human Jurkat; Human Jurkat. There is BioGPS gene expression data showing that NRF1 is expressed in Jurkat.; NRF1 Antibody - middle region (ARP34523_P050) in Human Jurkat cells using Western Blot
Transfected 293T; WB Suggested Anti-NRF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; NRF1 Antibody - middle region (ARP34523_P050) in Transfected 293T cells using Western Blot
Hum. Fetal Liver; NRF1 antibody - N-terminal region (ARP38542_P050) in Hum. Fetal Liver cells using Western Blot
Mouse endothelial; NRF1 antibody - N-terminal region (ARP38542_P050) validated by WB using Mouse endothelial cells at 1:500.; NRF1 antibody - N-terminal region (ARP38542_P050) in Mouse endothelial cells using Western Blot
Human 293T; WB Suggested Anti-NRF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T. There is BioGPS gene expression data showing that NRF1 is expressed in HEK293T.; NRF1 antibody - N-terminal region (ARP38542_P050) in Human 293T cells using Western Blot
Western blot analysis of extracts from 3T3 cells, using NRF1 antibody.The lane on the right is treated with the synthesized peptide.
WB using anti-NRF1 antibody shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 µg of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at RT for 1 h followed by detection using IRDye™700 labeled Goat-a-Rabbit IgG [H&L] (611-130-122) diluted 1:2,500.
Affinity Purified anti-NRF1antibody was used at a 5 µg/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Western blot analysis of extracts of various cell lines, using NRF1 antibody.
WB Suggested Anti-NRF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T
Human 293TThere is BioGPS gene expression data showing that NRF1 is expressed in HEK293T
Human HelaThere is BioGPS gene expression data showing that NRF1 is expressed in HeLa
Human JurkatThere is BioGPS gene expression data showing that NRF1 is expressed in Jurkat
Human Prostate
NRF1 antibody - N-terminal region validated by WB using Mouse endothelial cells at 1: 500.
Hum. Fetal Liver
WB Suggested Anti-NRF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T; There is BioGPS gene expression data showing that NRF1 is expressed in HEK293T
WB Suggested Anti-NRF1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T
Human Lung
Western blot detection of TCF11/NRF1 in Hela,K562,CEM and U2OS cell lysates using TCF11/NRF1 mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:72KDa.
Gel: 8%SDS-PAGE, Lysate: 30 ug, Lane: Hela cells, Primary antibody: (NRF1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319375 | NRF1 (1-534) antibody |
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| Rabbit polyclonal anti-NRF1 antibody | |||||||
![WB using anti-NRF1 antibody shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 µg of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at RT for 1 h followed by detection using IRDye™700 labeled Goat-a-Rabbit IgG [H&L] (611-130-122) diluted 1:2,500.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/200-401-869-1-W.jpg) |
Rabbit | IgG | Aff - Purified | Chimp, Chk, Frog, Hu, Ms, Rt, Ze | E, P, WB |
0.5 mg /
€590.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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