NSUN2 antibody
Principal name
NSUN2 antibody
Alternative names for NSUN2 antibody
SAKI, TRM4, tRNA (cytosine-5-)-methyltransferase NSUN2, NOL1/NOP2/Sun domain family member 2, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, Substrate of AIM1/Aurora kinase B
Ncbi ID
NP_060225, NP_001180384, NP_663329.3, NM_017755, NP_001180384.1, NM_145354
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of NSUN2 antibody
Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].
General readings
Huttenhofer,A., (2007) Mol. Biol. Cell 18 (3), 1107-1117
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52942PU-N | NSUN2 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP55409PU-N | NSUN2 antibody |
||||||
 |
Rabbit | Purified | Ms | C, E, WB |
0.1 mg /
€450.00
|
||
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN214459 | NSUN2 - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN214459BN | NSUN2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN214459LP | NSUN2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN214459RB | NSUN2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP314459 | NSUN2 |
||||
| Recombinant protein of human NOL1/NOP2/Sun domain family, member 2 (NSUN2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402617 | NSUN2 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220146L2V | Lenti ORF particles, Nsun2 (GFP-tagged) - Mouse NOL1/NOP2/Sun domain family member 2 (Nsun2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,550.00
|
|
| OriGene Technologies, Inc. | ||
| MR220146L1V | Lenti ORF particles, Nsun2 (Myc-DDK-tagged) - Mouse NOL1/NOP2/Sun domain family member 2 (Nsun2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,390.00
|
|
| OriGene Technologies, Inc. | ||