Nucleobindin-1 antibody
Principal name
Nucleobindin-1 antibody
Alternative names for Nucleobindin-1 antibody
NUCB1, NUC
Ncbi ID
9606, NP_006175, NP_445915, NP_006175.2, NP_445915.1, NP_001157134.1, NM_001163662, NM_008749, NM_053463, NM_006184
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)
Background of Nucleobindin-1 antibody
- Kit Component:
- - KN311307G1, Nucb1 gRNA vector 1 in pCas-Guide vector
- KN311307G2, Nucb1 gRNA vector 2 in pCas-Guide vector
- KN311307D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Huaxi Xu, et al., (2007) Journal of Neurochemistry 100:1505
Immunoperoxidase of monoclonal antibody to NUCB1 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 3ug/ml]
Immunofluorescence of monoclonal antibody to NUCB1 on HeLa cell. [antibody concentration 10 ug/ml]
NUCB1 monoclonal antibody (M01), clone 1H8-2C11 Western Blot analysis of NUCB1 expression in HepG2 ( Cat # L019V1 ).
Detection limit for recombinant GST tagged NUCB1 is approximately 0.1ng/ml as a capture antibody.
HepG2; Host:. Rabbit. Target Name:. NUCB1. Sample Tissue:. HepG2 lysates. Antibody Dilution: 1.0ug/ml; NUCB1 antibody - C-terminal region (P102230_T100) in HepG2 cells using Western Blot
Rat NRK; NUCB1 antibody - C-terminal region (P101699_T100) in Rat NRK cells using Immunohistochemistry
Human HeLa; WB Suggested Anti-NUCB1 Antibody Titration: 5.0ug/ml. Positive Control: Hela cell lysate; NUCB1 antibody - C-terminal region (P101699_T100) in Human HeLa cells using Western Blot
Human HepG2; WB Suggested Anti-NUCB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; NUCB1 antibody - C-terminal region (P102230_P050) in Human HepG2 cells using Western Blot
Western Blot analysis of NUCB1 expression in transfected 293T cell line (H00004924-T01) by NUCB1 MaxPab polyclonal antibody.Lane 1: NUCB1 transfected lysate(53.90 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503868)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503868)
Anti-NUCB1 mouse monoclonal antibody (TA503868) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1(RC201786).
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.
HEK293T cells transfected with either RC201786 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUCB1 antibody(TA503868), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NUCB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.
HEK293T cells transfected with either RC201786 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUCB1 antibody(TA503917), and then analyzed by flow cytometry.
Anti-NUCB1 mouse monoclonal antibody (TA503930) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1(RC201786).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504035)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504035)
Anti-NUCB1 mouse monoclonal antibody (TA504035) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1(RC201786).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.
WB Suggested Anti-NUCB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate
Host: Rabbit; Target Name: NUCB1; Sample Tissue:HepG2 lysates; Antibody Dilution: 1.0ug/ml
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503868 | Nucleobindin-1 antibody |
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| NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI1A5 (formerly 1A5) | |||||||
 |
Mouse | IgG1 | OTI1A5 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
||||||
| TA504035 | Nucleobindin-1 antibody |
||||||
| NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI1G3 (formerly 1G3) | |||||||
 |
Mouse | IgG1 | OTI1G3 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA503917 | Nucleobindin-1 antibody |
||||||
| NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI1A3 (formerly 1A3) | |||||||
 |
Mouse | IgG1 | OTI1A3 | Purified | Hu | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503930 | Nucleobindin-1 antibody |
||||||
| NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) | |||||||
 |
Mouse | IgG1 | OTI2A5 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301786 | Nucleobindin-1 |
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| Recombinant protein of human nucleobindin 1 (NUCB1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207335L2V | Lenti ORF particles, Nucb1 (GFP-tagged) - Mouse nucleobindin 1 (Nucb1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR216878L2V | Lenti ORF particles, Nucb1 (GFP-tagged) - Mouse nucleobindin 1 (Nucb1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||
| MR207335L1V | Lenti ORF particles, Nucb1 (Myc-DDK-tagged) - Mouse nucleobindin 1 (Nucb1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR216878L1V | Lenti ORF particles, Nucb1 (Myc-DDK-tagged) - Mouse nucleobindin 1 (Nucb1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,010.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201710L2V | Lenti ORF particles, Nucb1 (GFP-tagged ORF) - Rat nucleobindin 1 (Nucb1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||
| RR201710L1V | Lenti ORF particles, Nucb1 (Myc-DDK-tagged ORF) - Rat nucleobindin 1 (Nucb1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,010.00
|
|
| OriGene Technologies, Inc. | ||