Nucleostemin antibody
Principal name
Nucleostemin antibody
Alternative names for Nucleostemin antibody
GNL3, E2IG3, NS, Guanine nucleotide-binding protein-like 3, Nucleolar GTP-binding protein 3, Novel nucleolar protein 47, E2-induced gene 3 protein
Ncbi ID
NP_055181, 9606, NP_996561, NM_206826, NM_014366, NP_055181.3, NP_705775.2, NM_175580, NM_206825
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of Nucleostemin antibody
- Kit Component:
- - KN307095G1, Gnl3 gRNA vector 1 in pCas-Guide vector
- KN307095G2, Gnl3 gRNA vector 2 in pCas-Guide vector
- KN307095D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 polyclonal antibody ( Cat # PAB2481 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of GNL3 polyclonal antibody ( Cat # PAB2482 ) in HL-60 cell line lysate ( 35 µg/lane ) . GNL3 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody (C-term) (cat.#AP11354PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-GNL3 Antibody (Center) (Cat#AP11355PU-N) in HL60 cell line lysates (35ug/lane). GNL3 (arrow) was detected using the purified Pab (1:60 dilution).
Immunofluorescence of purified MaxPab antibody to GNL3 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of GNL3 expression in transfected 293T cell line (H00026354-T01) by GNL3 MaxPab polyclonal antibody.Lane 1: GNL3 transfected lysate(60.39 KDa).Lane 2: Non-transfected lysate.
GNL3 MaxPab polyclonal antibody. Western Blot analysis of GNL3 expression in LNCaP.
GNL3 MaxPab polyclonal antibody. Western Blot analysis of GNL3 expression in HeLa.
Human 721_B; WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. GNL3 is supported by BioGPS gene expression data to be expressed in 721_B.; GNL3 antibody - middle region (ARP58827_P050) in Human 721_B cells using Western Blot
Human Stomach; WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Stomach; GNL3 antibody - N-terminal region (ARP58826_P050) in Human Stomach cells using Western Blot
TA334188 (0.03µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysate. GNL3 is supported by BioGPS gene expression data to be expressed in 721_B
WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Stomach
TA348931 (2 ug/ml) staining of HeLa (A) HepG2 (B) and K562 (C) nuclear lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: K562 cells, Hela cells, LoVo cells, 293T cells, Raji cells, Primary antibody: (GNL3 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (GNL3 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (GNL3 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA334188 | Nucleostemin (115-126) antibody |
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| Goat Anti-GNL3 (aa115-126) Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA348931 | Nucleostemin antibody |
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| Goat Polyclonal Anti-GNL3 Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350242 | Nucleostemin antibody |
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| Rabbit Polyclonal Anti-GNL3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP11354PU-N | Nucleostemin (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP11355PU-N | Nucleostemin antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| GT15050-100 | Nucleostemin antibody |
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 |
Goat | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€450.00
|
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| Neuromics Antibodies | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN200066 | GNL3 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200066BN | GNL3 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200066LP | GNL3 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200066RB | GNL3 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760526 | Nucleostemin (full length, N-term HIS tag, transcript variant 1) |
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| Purified recombinant protein of Human guanine nucleotide binding protein-like 3 (nucleolar) (GNL3), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY404228 | GNL3 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY415330 | GNL3 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY430949 | GNL3 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR210460L2V | Lenti ORF particles, Gnl3 (GFP-tagged ORF) - Rat guanine nucleotide binding protein-like 3 (nucleolar) (Gnl3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| RR210460L1V | Lenti ORF particles, Gnl3 (Myc-DDK-tagged ORF) - Rat guanine nucleotide binding protein-like 3 (nucleolar) (Gnl3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
