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NUP160 antibody

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Principal name

NUP160 antibody

Alternative names for NUP160 antibody

KIAA0197, NUP120, Nucleoporin Nup160, Nuclear pore complex protein Nup160, 160 kDa nucleoporin

SwissProt ID

P83722 (Xenla), Q12769 (Human), Q9Z0W3 (Mouse)

Gene ID

23279 (NUP160), 59015 (Nup160)

Ncbi ID

NP_056046.1, 9606, NP_056046, NM_015231, NP_067487.1, NM_021512

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NUP160 antibody

The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-160 is part of the hetero-oligomeric complex that also contains NUP107, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP160 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP160 may be an important drug target in HIV treatment. Multiple isoforms of NUP160 are known to exist.

General readings

Mishra, R.K., et al. Nat. Cell Biol. 12(2):164-169(2010)
Platani, M., et al. Mol. Biol. Cell 20(24):5260-5275(2009)
Leksa, N.C., et al. Structure 17(8):1082-1091(2009)
Zuccolo, M., et al. EMBO J. 26(7):1853-1864(2007)
Glavy, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 104(10):3811-3816(2007)

AP01491PU-N - NUP160
Western blot (WB) analysis of Nup160 antibody (Cat.-No.: AP01491PU-N) in extracts from HepG2 cells.
PAB13052 - NUP160
Western blot analysis of NUP160 in rat brain tissue lysate with NUP160 polyclonal antibody ( Cat # PAB13052 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
C17093-1 - NUP160
Western blot analysis of extracts from NIH-3T3/A549/HepG2 cells, using NUP160 Antibody. The lane on the right is treated with the synthesized peptide.
AP52971PU-N - NUP160
Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue reacted with NUP160 Antibody (Center) Cat.-No AP52971PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
AP52971PU-N - NUP160
Western blot analysis of NUP160 Antibody (Center) Cat.-No AP52971PU-N in mouse NIH-3T3 cell line lysates (35ug/lane). This demonstrates the NUP160 antibody detected the NUP160 protein (arrow).
CPA2571-100ul - NUP160
Immunofluorescent analysis of NUP160 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA2571-100ul - NUP160
Western blot analysis of NUP160 expression in HeLa (A); NIH3T3 (B) whole cell lysates.
TA306614 - NUP160
Western blot analysis of NUP160 in rat brain tissue lysate with NUP160 antibody at (A) 0.5 and (B) 1 ug/ml.
TA306614 - NUP160
Immunohistochemistry of NUP160 in human brain tissue with NUP160 antibody at 2.5 ug/ml.
TA306614 - NUP160
Immunofluorescence of NUP160 in Human Brain cells with NUP160 antibody at 20 ug/mL.
TA312716 - NUP160
Western blot analysis of extracts from HepG2 cells, 3T3 cells and A549 cells, using NUP160 antibody.The lane on the right is treated with the synthesized peptide.

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NUP160 (C-term) antibody
  Rabbit Polyclonal NUP160 Antibody  
Western blot analysis of NUP160 in rat brain tissue lysate with NUP160 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

NUP160 antibody
  Rabbit polyclonal anti-NUP160 antibody  
Western blot analysis of extracts from HepG2 cells, 3T3 cells and A549 cells, using NUP160 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

NUP160 antibody
Western blot (WB) analysis of Nup160 antibody (Cat.-No.: AP01491PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €370.00
  OriGene Technologies GmbH

NUP160 (Center) antibody
Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue reacted with NUP160 Antibody (Center) Cat.-No AP52971PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.    

NUP160 overexpression lysate
NUP160 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nup160 (GFP-tagged) - Mouse nucleoporin 160 (Nup160), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nup160 (GFP-tagged) - Mouse nucleoporin 160 (Nup160), 200 uL, >10^7 TU/mL  
200 µl / €2,450.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Nup160 (Myc-DDK-tagged) - Mouse nucleoporin 160 (Nup160), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nup160 (Myc-DDK-tagged) - Mouse nucleoporin 160 (Nup160), 200 uL, >10^7 TU/mL  
200 µl / €2,210.00
  OriGene Technologies, Inc.
 

7 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (4)
Clonality
  1. Polyclonal (4)
Target
  1. NUP160 (4)
Property
  1. C-term (1)
  2. Center (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (2)
  3. Paraffin Sections (P) (2)
Presentation
  1. Aff - Purified (3)
  2. Purified (1)
Host
  1. Rabbit (4)
Images
  1. 1 image (4)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunofluorescence (IF) (1)
  3. IHC (1)
  4. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (7)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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