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NUP50 antibody

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Principal name

NUP50 antibody

Alternative names for NUP50 antibody

Nucleoporin 50 kDa, NPAP60L, Nuclear pore-associated protein 60 kDa-like

SwissProt ID

O08587 (Rat), Q9JIH2 (Mouse), Q9UKX7 (Human)

Gene ID

10762 (NUP50), 25497 (Npap60)

Ncbi ID

9606, NP_009103, NP_009103.2, NM_153645, NP_705931, NP_037123.1, NM_012991, NM_007172

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of NUP50 antibody

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Ogawa, Y., et al. Mol. Biol. Cell 21(4):630-638(2010)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)

PAB3709 - NUP50
Western blot analysis of NUP50 ( arrow ) usingNUP50 polyclonal antibody ( Cat # PAB3709 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the NUP50 gene ( Lane 2 ) ( Origene Technologies ) .
PAB3709 - NUP50
Western blot analysis of NUP50 polyclonal antibody ( Cat # PAB3709 ) in mouse brain tissue lysate. NUP50 ( arrow ) was detected using the purified polyclonal antibody.
PAB6369 - NUP50
NUP50 polyclonal antibody ( Cat # PAB6369 ) staining ( 0.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
H00010762-B01 - NUP50
Immunofluorescence of purified MaxPab antibody to NUP50 on HeLa cell. [antibody concentration 10 ug/ml]
H00010762-B01 - NUP50
NUP50 MaxPab polyclonal antibody. Western Blot analysis of NUP50 expression in human liver.
H00010762-B01 - NUP50
Western Blot analysis of NUP50 expression in transfected 293T cell line (H00010762-T01) by NUP50 MaxPab polyclonal antibody.Lane 1: NUP50 transfected lysate(51.48 KDa).Lane 2: Non-transfected lysate.
H00010762-B01P - NUP50
Immunofluorescence of purified MaxPab antibody to NUP50 on HeLa cell. [antibody concentration 10 ug/ml]
H00010762-B01P - NUP50
NUP50 MaxPab polyclonal antibody. Western Blot analysis of NUP50 expression in human liver.
H00010762-B01P - NUP50
Western Blot analysis of NUP50 expression in transfected 293T cell line by NUP50 MaxPab polyclonal antibody.Lane 1: NUP50 transfected lysate(51.48 KDa).Lane 2: Non-transfected lysate.
ARP52288_P050 - NUP50
Human HeLa; . Sample Type : Hela cell lysate .; NUP50 antibody - C-terminal region (ARP52288_P050) in Human HeLa cells using Immunofluorescence
ARP52288_P050 - NUP50
Human Hela; Host: Rabbit . Target Name: NUP50 . Sample Tissue: Hela . Antibody Dilution: 1.0ug/ml. NUP50 is supported by BioGPS gene expression data to be expressed in HeLa.; NUP50 antibody - C-terminal region (ARP52288_P050) in Human Hela cells using Western Blot
ARP52288_P050 - NUP50
Human HepG2; WB Suggested Anti-NUP50 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that NUP50 is expressed in HepG2.; NUP50 antibody - C-terminal region (ARP52288_P050) in Human HepG2 cells using Western Blot
ARP52288_P050 - NUP50
Human HepG2; Host: Rabbit . Target Name: NUP50 . Sample Tissue: HepG2 . Antibody Dilution: 1.0ug/ml. There is BioGPS gene expression data showing that NUP50 is expressed in HepG2.; NUP50 antibody - C-terminal region (ARP52288_P050) in Human HepG2 cells using Western Blot
AP11702PU-N - NUP50
Western blot analysis of NUP50 (arrow) using rabbit polyclonal NUP50 Antibody (C-term) (AP11702PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies).
AP11702PU-N - NUP50
Western blot analysis of anti-NUP50 (AP11702PU-N) in mouse brain tissue lysate. NUP50 (arrow) was detected using the purified Pab.
AP52974PU-N - NUP50
Western blot analysis of NUP50 Antibody (Center) Cat.-No AP52974PU-N in WiDr cell line lysates (35ug/lane). This demonstrates the NUP50 antibody detected the NUP50 protein (arrow).
TP307001 - NUP50
TP309975 - NUP50
TA302862 - NUP50
TA302862 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA340153 - NUP50
Host: Rabbit; Target Name: Npap60; Sample Tissue: Rat Brain lysates; Antibody Dilution: 1.0 ug/ml
TA340154 - NUP50
Host: Rabbit; Target Name: NUP50; Sample Tissue: HepG2; Antibody Dilution: 1.0 ug/mlThere is BioGPS gene expression data showing that NUP50 is expressed in HepG2
TA340154 - NUP50
WB Suggested Anti-NUP50 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that NUP50 is expressed in HepG2
TA340154 - NUP50
Host: Rabbit; Target Name: NUP50; Sample Tissue: Hela; Antibody Dilution: 1.0 ug/mlNUP50 is supported by BioGPS gene expression data to be expressed in HeLa
TA340154 - NUP50
Sample Type: Hela cell lysate;
TA350245 - NUP50
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-5: Hela cells, 293T cells, A549 cells, HT29 cells, K562 cells, Primary antibody: (NUP50 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
TA350245 - NUP50
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using (NUP50 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA350245 - NUP50
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (NUP50 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NUP50 (C-term) antibody
  Goat Polyclonal Antibody against NUP50  
TA302862 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

NUP50 antibody
  Rabbit Polyclonal Anti-NUP50 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-5: Hela cells, 293T cells, A549 cells, HT29 cells, K562 cells, Primary antibody: (NUP50 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

NUP50 (C-term) antibody
Western blot analysis of NUP50 (arrow) using rabbit polyclonal NUP50 Antibody (C-term) (AP11702PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

NUP50 (Center) antibody
Western blot analysis of NUP50 Antibody (Center) Cat.-No AP52974PU-N in WiDr cell line lysates (35ug/lane). This demonstrates the NUP50 antibody detected the NUP50 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NUP50 (transcript variant 3)
  Recombinant protein of human nucleoporin 50kDa (NUP50), transcript variant 3  
NUP50 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

NUP50 (transcript variant 2)
  Recombinant protein of human nucleoporin 50kDa (NUP50), transcript variant 2  
NUP50 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

NUP50 overexpression lysate
NUP50 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

NUP50 overexpression lysate
NUP50 overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Npap60 (GFP-tagged ORF) - Rat nuclear pore associated protein (Npap60), 200 uL, >10^7 TU/mL
Lenti ORF particles, Npap60 (GFP-tagged ORF) - Rat nuclear pore associated protein (Npap60), 200 uL, >10^7 TU/mL  
200 µl / €1,150.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Npap60 (Myc-DDK-tagged ORF) - Rat nuclear pore associated protein (Npap60), 200 uL, >10^7 TU/mL
Lenti ORF particles, Npap60 (Myc-DDK-tagged ORF) - Rat nuclear pore associated protein (Npap60), 200 uL, >10^7 TU/mL  
200 µl / €1,020.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. Lysate (2)
  2. Primary Antibody (4)
  3. Protein | Growth Factor (2)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
Target
  1. NUP50 (6)
Property
  1. C-term (2)
  2. Center (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (2)
  3. Bovine (Bov) (1)
  4. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Paraffin Sections (P) (2)
  3. Enzyme Immunoassay (E) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (2)
Host
  1. Rabbit (3)
  2. Goat (1)
Images
  1. 1 image (6)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. IHC (1)
Species (Protein)
  1. Human (2)
Source
  1. HEK293 cells (2)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
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