Occludin antibody
Principal name
Occludin antibody
Alternative names for Occludin antibody
OCLN
SwissProt ID
Q16625 (Human), Q28269 (Canfa), Q61146 (Mouse), Q6P6T5 (Rat), Q91049 (Chick), Q9PUN1 (Xenla)
Ncbi ID
9606, NP_002529, NP_001192183.1, NP_002529.1, NM_002538, AAH29886, NP_032782.1, NM_008756, NM_031329
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)
Background of Occludin antibody
- Kit Component:
- - KN311431G1, Ocln gRNA vector 1 in pCas-Guide vector
- KN311431G2, Ocln gRNA vector 2 in pCas-Guide vector
- KN311431D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Shen,L., (2008) J. Cell Biol. 181 (4), 683-695
Immunofluorescence of monoclonal antibody to OCLN on HeLa cell. [antibody concentration 10 ug/ml]
OCLN monoclonal antibody (M01), clone 1G7 Western Blot analysis of OCLN expression in HL-60 ( Cat # L014V1 ).
Detection limit for recombinant GST tagged OCLN is approximately 0.1ng/ml as a capture antibody.
OCLN polyclonal antibody (A01), Lot # 051207JC01 Western Blot analysis of OCLN expression in Jurkat ( Cat # L017V1 ).
Proximity Ligation Analysis (PLA) of protein-protein interactions between OCLN and CSNK1E. HeLa cells were stained with anti-OCLN rabbit purified polyclonal 1:1200 and anti-CSNK1E mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
OCLN MaxPab rabbit polyclonal antibody. Western Blot analysis of OCLN expression in mouse liver.
OCLN MaxPab rabbit polyclonal antibody. Western Blot analysis of OCLN expression in human liver.
Western Blot analysis of OCLN expression in transfected 293T cell line (H00004950-T01) by OCLN MaxPab polyclonal antibody.Lane 1: OCLN transfected lysate(59.10 KDa).Lane 2: Non-transfected lysate.
Transfected 293T; WB Suggested Anti-OCLN Antibody Titration: 2.5ug/ml. Positive Control: Transfected 293T; OCLN antibody - N-terminal region (ARP42888_T100) in Transfected 293T cells using Western Blot
Human Lung stained with Anti-Occludin antibody Cat.-No AP15690PU
Western Blot analysis of kidney cell lysate with occluding antibody Cat.-No AP15690PU
Western Blot analysis of OCLN expression in transfected 293T cell line (H00004950-T01) by OCLN MaxPab polyclonal antibody.Lane 1: OCLN transfected lysate(57.42 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of OCLN on formalin fixed, paraffin embedded human lung with OCLN polyclonal antibody.
Human COLO205; WB Suggested Anti-OCLN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate; OCLN antibody - N-terminal region (ARP42889_P050) in Human COLO205 cells using Western Blot
Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 ug/ml.
Immunohistochemistry of OCLN in human liver tissue with OCLN antibody at 2.5 ug/ml.
Immunofluorescence of OCLN in Human Liver tissue with OCLN antibody at 20 ug/mL.
Predicted band size: 59 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)
Predicted cell location: Cytoplasm . Positive control: Human liver cancer tissue . Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using OCLN antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Cytoplasm . Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using OCLN antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm . Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using OCLN antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306787 | Occludin antibody |
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| Rabbit Polyclonal OCLN Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA324148 | Occludin antibody |
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| Anti-OCLN Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA324149 | Occludin antibody |
||||||
| Anti-OCLN Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP26410PU-N | Occludin (358-504) antibody |
||||||
 |
Guinea Pig | Ig | Purified | Can, Hu, Ms, Rt | C, ICC/IF, WB |
0.1 mg /
€390.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306468 | Occludin |
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| Recombinant protein of human occludin (OCLN) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419249 | OCLN overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209068L2V | Lenti ORF particles, Ocln (GFP-tagged ORF) - Rat occludin (Ocln), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,220.00
|
|
| OriGene Technologies, Inc. | ||
| RR209068L1V | Lenti ORF particles, Ocln (Myc-DDK-tagged ORF) - Rat occludin (Ocln), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,090.00
|
|
| OriGene Technologies, Inc. | ||