OGG1 antibody
Principal name
OGG1 antibody
Alternative names for OGG1 antibody
MMH, MUTM, OGH1, N-glycosylase/DNA lyase
Ncbi ID
NP_110497.1, NP_002533, 9606, 10090, NP_002533.1, NM_016819, NP_058214, NP_035087.3, NM_010957, NM_030870
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)
Background of OGG1 antibody
The Ogg1 gene encodes a DNA repair enzyme (N glycosylase/DNA lyase) that incises DNA at 8 oxoG residues. N glycosylase/DNA lyase excises 7,8 dihydro 8 oxoguanine and 2,6 diamino 4 hydroxy 5 N methylformamidopyrimidine (FAPY) from damaged DNA. N glycosylase/DNA lyase has a beta lyase activity that nicks DNA 3' to the lesion.
Alternative splicing of the C terminal region of the Ogg1 gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N terminal region in common. Many alternative splice variants for this gene have been described, but the full length nature for every variant has not been determined. The N terminus of this gene contains a mitochondrial targetting signal, essential for mitochondrial localization.
General readings
Seeberg E., et al. (2002). Nucleic Acids Res. 30(11):2349-57.
Hodges NJ., et al (2010). DNA Repair (Amst). 9(2):144-52.
Figure 1. Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded Kidney tissue using AP08789PU-N at 2.5 µg/ml.
Figure 2. Antibody (AP08789PU-N at 0.1 µg/ml) staining of Rat Spleen lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by Chemiluminescence.
Western blot analysis of OGG1 Antibody (C-term) (AP17606PU-N) in Hela, NIH-3T3 cell line lysates (35ug/lane). OGG1 (arrow) was detected using the purified Pab.
Recombinant human OGG1, 1-345aa, His-tagged
Immunofluorescence of purified MaxPab antibody to OGG1 on HeLa cell. [antibody concentration 10 ug/ml]
OGG1 MaxPab polyclonal antibody. Western Blot analysis of OGG1 expression in human kidney.
Western Blot analysis of OGG1 expression in transfected 293T cell line (H00004968-T01) by OGG1 MaxPab polyclonal antibody.Lane 1: OGG1 transfected lysate(35.64 KDa).Lane 2: Non-transfected lysate.
OGG1 MaxPab rabbit polyclonal antibody. Western Blot analysis of OGG1 expression in Jurkat.
Western Blot analysis of OGG1 expression in transfected 293T cell line (H00004968-T01) by OGG1 MaxPab polyclonal antibody.Lane 1: OGG1 transfected lysate(36.40 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of OGG1 transfected lysate using anti-OGG1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with OGG1 MaxPab mouse polyclonal antibody (B01) (H00004968-B01).
Western Blot analysis of OGG1 expression in transfected 293T cell line (H00004968-T01) by OGG1 MaxPab polyclonal antibody.Lane 1: OGG1 transfected lysate(36.40 KDa).Lane 2: Non-transfected lysate.
OGG1 MaxPab rabbit polyclonal antibody. Western Blot analysis of OGG1 expression in Jurkat.
Immunohistochemical staining of OGG1 on formaldehyde fixed frozen sections of the substantia nigra from a Rhesus macaque with OGG1 polyclonal antibody ( Cat # PAB12085 ). Photo courtesy of Glen Kisby, Oregon Health Sciences University. The tissue was a generous gift of Dr. Steven Kohama, Oregon National Primate Research Center.
Western blot analysis of OGG1 of 1, 5, 10 ng titration of human recombinant Ogg1 protein with OGG1 polyclonal antibody ( Cat# PAB12085 ).
Western blot analysis of OGG1 in HeLa and Jurkat cell lysate with OGG1 polyclonal antibody ( Cat# PAB12085 ).
The Ogg1 polyclonal antibody ( Cat # PAB7311 ) ( 0.1 µg/ml ) staining of rat spleen lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
TA305839 (0.1ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Ogg1 antibody - 1, 5, 10 ng titration of human recombinant Ogg1 protein, detected by anti-Ogg1.
Western Blot: Ogg1 antibody
Figure from citation: Western blot analysis of OGG1 protein expression by using anti-OGG1 antibody in oak-initiated Beas-2B cells. View Citation
Immunohistochemistry-frozen: Ogg1 antibody - Detection of OGG1 in formaldehyde fixed frozen sections of the substantia nigra from a Rhesus macaque (Macaca mulatta) using anti-OGG1 at 10 ug/ml. Photo courtesy of Glen Kisby, Oregon Health Sciences University. The tissue was a generous gift of Dr. Steven Kohama, Oregon National Primate Research Center.
Immunohistochemistry: Ogg1 antibody - Staining of tonsil, germinal center and mantle zone using anti-Ogg1.
Predicted band size: 39 kDa . Positive control: Human heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309779 | OGG1 antibody |
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| Rabbit Polyclonal Ogg1 Antibody | |||||||
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Rabbit | Purified | Hu, Prim, Rt | C, ICC/IF, WB |
0.1 ml /
€475.00
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA305839 | OGG1 antibody |
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| Goat Anti-Ogg1 (mouse) Antibody | |||||||
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Goat | Aff - Purified | Ms, Rt | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA321496 | OGG1 antibody |
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| Anti-OGG1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| AP08789PU-N | OGG1 antibody |
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Goat | IgG | Aff - Purified | Hu, Ms | E, P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP17606PU-N | OGG1 (C-term) antibody |
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Rabbit | Hu, Ms | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP720109 | OGG1 (transcript variant 1a) |
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| Recombinant protein of human 8-oxoguanine DNA glycosylase (OGG1), nuclear gene encoding mitochondrial protein, transcript variant 1a | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
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| OriGene Technologies, Inc. | |||||
| TP760967 | OGG1 (full length, N-term HIS tag, transcript variant 2a) |
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| Purified recombinant protein of Human 8-oxoguanine DNA glycosylase (OGG1), nuclear gene encoding mitochondrial protein, transcript variant 2a, full length, with N-terminal HIS tag, expressed in E. coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY413835 | OGG1 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. | ||
| LY429520 | OGG1 overexpression lysate |
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0.1 mg /
€315.00
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| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR208594L2V | Lenti ORF particles, Ogg1 (GFP-tagged ORF) - Rat 8-oxoguanine DNA glycosylase (Ogg1), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| RR208594L1V | Lenti ORF particles, Ogg1 (Myc-DDK-tagged ORF) - Rat 8-oxoguanine DNA glycosylase (Ogg1), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||