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ORAI1 antibody

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Principal name

ORAI1 antibody

Alternative names for ORAI1 antibody

ORAI-1, CRACM1, TMEM142A, Protein orai-1, Transmembrane protein 142A, Calcium release-activated calcium channel protein 1

SwissProt ID

Q5M848 (Rat), Q5ZL05 (Chick), Q8BWG9 (Mouse), Q96D31 (Human)

Gene ID

84876 (ORAI1), 109305 (Orai1), 304496

Ncbi ID

NP_116179.2, NP_116179, 9606, Q96D31, NP_780632.1, BC023149, NM_175423, NM_001013982, NM_032790

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Bt (Bat), Eq (Equine), Hst (Hamster), Mky (Monkey), Op (Opossum), Prim (Primate), Rb (Rabbit), Bear, Ye (Yeast)

Available hosts

Rabbit, Goat, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of ORAI1 antibody

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including syptic transmission, muscle contraction, hormol secretion, cell growth and proliferation. Intracellular Ca2+ levels are controlled by either the influx of Ca2+ through the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores which gained much attention. Recently, several key players of the store operated complex have been identified. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor. The formation of functiol channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit. Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai3. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes.

General readings

Vig M, DeHaven WI, Bird GS, Billingsley JM, Wang H, Rao PE, Hutchings AB, Jouvin MH, Putney JW, Kinet JP.
Defective mast cell effector functions in mice lacking the CRACM1 pore subunit of store-operated calcium release-activated calcium channels.
Nat Immunol. 2008 Jan;9(1):89-96. Epub 2007 Dec 2.
PMID: 18059270

AP05736PU-N - ORAI1
Immunohistochemical staining of human spleen with Rabbit anti Human ORAI1 antibody Cat.-No AP05736PU-N
AP05736PU-N - ORAI1
Western blot analysis of human ovary probed with Rabbit anti Human ORAI1 antibody Cat.-No AP05736PU-N at 0.5(A), 1(B) and 2(C) µg/ml
ARP50117_P050 - ORAI1
Human HepG2; WB Suggested Anti-ORAI1 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ORAI1 antibody - N-terminal region (ARP50117_P050) in Human HepG2 cells using Western Blot
ARP50118_P050 - ORAI1
Human HepG2; WB Suggested Anti-ORAI1 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ORAI1 antibody - middle region (ARP50118_P050) in Human HepG2 cells using Western Blot
PAB11461 - ORAI1
ORAI1 polyclonal antibody ( Cat # PAB11461 ) ( 0.03 µg/ml ) staining of human placenta lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
PAB13248 - ORAI1
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 polyclonal antibody ( Cat # PAB13248 ) at 10 µg/ml.
PAB13248 - ORAI1
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 polyclonal antibody ( Cat # PAB13248 ) at 1 µg/ml in the ( A ) absence or ( B ) presence of blocking peptide.
PAB13282 - ORAI1
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 polyclonal antibody ( Cat # PAB13282 ) at 2.5 µg/ml.
PAB13282 - ORAI1
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 polyclonal antibody ( Cat # PAB13282 ) at ( A ) 0.5, ( B ) 1 and ( C ) 2 µg/ml.
AP23057PU-N - ORAI1
Immunohistochemistry: Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE).
AP53115PU-N - ORAI1
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ORAI1 Antibody (Center) Cat.-No AP53115PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP53115PU-N - ORAI1
Western blot analysis of ORAI1 Antibody (Center) Cat.-No AP53115PU-N in A375 cell line and mouse testis tissue lysates (35ug/lane). ORAI1 (arrow) was detected using the purified Pab.
TA305897 - ORAI1
TA305897 (0.03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA306394 - ORAI1
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.
TA306394 - ORAI1
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.
TA306394 - ORAI1
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/mL.
TA306452 - ORAI1
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
TA306452 - ORAI1
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 ug/ml.
TA320059 - ORAI1
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL.
TA320059 - ORAI1
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 ug/mL.
TA320059 - ORAI1
Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 ug/mL.
TA320060 - ORAI1
Western blot analysis of ORAI1 in human spleen tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL.
TA320060 - ORAI1
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 ug/mL.
TA320060 - ORAI1
Immunofluorescence of ORAI1 in Human Spleen tissue with ORAI1 antibody at 20 ug/mL.
TA328653 - ORAI1
Western blot analysis of Jurkat (lanes 1,2) and HL-60 (lanes 3,4) cell lysates: 1, 3. Anti-Human Orai1 (extracellular) antibody, (1:200).2, 4. Anti-Human Orai1 (extracellular) antibody, preincubated with the control peptide antigen.
TA328653 - ORAI1
Indirect flow cytometry analysis of intact HL-60 (A) and Jurkat cells (B ).black line, Unstained cells.green line, Cells + Anti-Human Orai1 (extracellular) antibody, (5-10 µg antibody/0.5-1 x 106 cells).
TA328752 - ORAI1
Western blot analysis of rat spleen (lanes 1 and4), rat pancreas (lanes 2 and 5) and mouse B-cell lymphoma (WEHI) (lanes 3 and 6) lysates: 1-3. Anti-Orai1 (extracellular) antibody, (1:200). 4-6. Anti-Orai1 (extracellular) antibody, preincubated with the control peptide antigen.
TA328752 - ORAI1
Expression of Orai1 in rat pancreas.Immunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Orai1 (extracellular) antibody, (1:50). Orai1 (brown) is expressed in acinar cells. Hematoxilin is used as the counterstain.
TA328752 - ORAI1
Expression of Orai1 in rat RBL cells.Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-Orai1 (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Merge of A and Live view of the cells.
TA328752 - ORAI1
Indirect flow cytometry analysis of WEHI living cells: black line, Unstained cells. green line, Cells + Anti-Orai1 (extracellular) antibody, (10 µg/0.5 x 106 cells).
TA339627 - ORAI1
WB Suggested Anti-ORAI1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
TA339628 - ORAI1
WB Suggested Anti-ORAI1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate

12 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ORAI1 antibody
  Goat Anti-ORAI1 / CRACM1 Antibody  
TA305897 (0.03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

ORAI1 (N-term) antibody
  Rabbit Polyclonal ORAI1 Antibody  
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

ORAI1 (C-term) antibody
  Rabbit Polyclonal ORAI1 Antibody  
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

ORAI1 antibody
  Mouse ORAI1 Monoclonal Antibody  
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL. Mouse IgG 3F6H5 Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

ORAI1 antibody
  Mouse ORAI1 Monoclonal Antibody  
Western blot analysis of ORAI1 in human spleen tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL. Mouse IgG 6D11A11 Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

ORAI1 antibody
  Rabbit Polyclonal Anti-Human Orai1 (extracellular)  
Western blot analysis of Jurkat (lanes 1,2) and HL-60 (lanes 3,4) cell lysates: 1, 3. Anti-Human Orai1 (extracellular) antibody, (1:200).2, 4. Anti-Human Orai1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu WB
0.2 ml / €585.00
  OriGene Technologies, Inc.
+1 additional image

ORAI1 antibody
  Rabbit Polyclonal Anti-Orai1 (extracellular)  
Western blot analysis of rat spleen (lanes 1 and4), rat pancreas (lanes 2 and 5) and mouse B-cell lymphoma (WEHI) (lanes 3 and 6) lysates: 1-3. Anti-Orai1 (extracellular) antibody, (1:200). 4-6. Anti-Orai1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €585.00
  OriGene Technologies, Inc.
+3 additional images

ORAI1 (Center) antibody
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ORAI1 Antibody (Center) Cat.-No AP53115PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Tmem142a (GFP-tagged) - Mouse transmembrane protein 142A (cDNA clone MGC:28390 IMAGE:4022503), 200 uL, >10^7 TU/mL
Lenti ORF particles, Tmem142a (GFP-tagged) - Mouse transmembrane protein 142A (cDNA clone MGC:28390 IMAGE:4022503), 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Tmem142a (Myc-DDK-tagged) - Mouse transmembrane protein 142A (cDNA clone MGC:28390 IMAGE:4022503), 200 uL, >10^7 TU/mL
Lenti ORF particles, Tmem142a (Myc-DDK-tagged) - Mouse transmembrane protein 142A (cDNA clone MGC:28390 IMAGE:4022503), 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Orai1 (GFP-tagged ORF) - Rat ORAI calcium release-activated calcium modulator 1 (Orai1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Orai1 (GFP-tagged ORF) - Rat ORAI calcium release-activated calcium modulator 1 (Orai1), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Orai1 (Myc-DDK-tagged ORF) - Rat ORAI calcium release-activated calcium modulator 1 (Orai1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Orai1 (Myc-DDK-tagged ORF) - Rat ORAI calcium release-activated calcium modulator 1 (Orai1), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

12 Item(s)

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12 products - FILTER YOUR RESULTS!

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  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (8)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (6)
  2. Monoclonal (2)
Target
  1. ORAI1 (8)
Property
  1. C-term (1)
  2. Center (1)
  3. N-term (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (7)
  3. Rat (Rt) (4)
Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Paraffin Sections (P) (5)
  3. Enzyme Immunoassay (E) (4)
Presentation
  1. Aff - Purified (6)
  2. Purified (2)
Host
  1. Rabbit (5)
  2. Mouse (2)
  3. Goat (1)
Images
  1. 1 image (8)
  2. 3+ images (4)
Images: Applications
  1. Western blot / Immunoblot (WB) (8)
  2. IHC (5)
  3. Immunofluorescence (IF) (4)
  4. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (12)
Service
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