ORAOV1 antibody
Principal name
ORAOV1 antibody
Alternative names for ORAOV1 antibody
TAOS1, Oral cancer-overexpressed protein 1, Tumor-amplified and overexpressed sequence 1
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of ORAOV1 antibody
- Kit Component:
- - KN312623G1, Oraov1 gRNA vector 1 in pCas-Guide vector
- KN312623G2, Oraov1 gRNA vector 2 in pCas-Guide vector
- KN312623D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Xavier F C., et al. (2011) J Oral Pathol Med. 28:10.Jiang L., et al. (2010) Mol Cancer. 28: 20.
Immunohistochemical analysis of ORAOV1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ORAOV1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ORAOV1 expression in HeLa (A) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ORAOV1 Rabbit Polyclonal antibody. (TA590385)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ORAOV1 (RC208992, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ORAOV1.
ELISA: ORAOV1 Antibody
Western blot analysis of extracts from 293 cells and HeLa cells, using ORAV1 antibody.The lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA316247 | ORAOV1 antibody |
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| Rabbit polyclonal anti-ORAV1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201082L2V | Lenti ORF particles, Oraov1 (GFP-tagged) - Mouse oral cancer overexpressed 1 (Oraov1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201082L1V | Lenti ORF particles, Oraov1 (Myc-DDK-tagged) - Mouse oral cancer overexpressed 1 (Oraov1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||