OSBPL11 antibody
Principal name
OSBPL11 antibody
Alternative names for OSBPL11 antibody
OSBP-related protein 11, ORP-11, ORP11, OSBP12, Oxysterol-binding protein-related protein 11
Gene ID
114885 (OSBPL11)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of OSBPL11 antibody
- Kit Component:
- - KN209418G1, OSBPL11 gRNA vector 1 in pCas-Guide vector
- KN209418G2, OSBPL11 gRNA vector 2 in pCas-Guide vector
- KN209418D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Peptide ELISA: Limit dilution 1:16000.
Western blot: 1-2 µg/ml. Approx 85-90kDa band observed in Human Brain lysates (predicted MWs of 86kDa according to NP_073613). A minor band of unknown identity was also consistently observed at 75kDa.
OSBPL11 polyclonal antibody ( Cat # PAB6521 ) staining ( 1 µg/ml ) of human brain lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Human MCF-7; WB Suggested Anti-OSBPL11 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate. OSBPL11 is supported by BioGPS gene expression data to be expressed in MCF7.; OSBPL11 antibody - C-terminal region (ARP45658_P050) in Human MCF-7 cells using Western Blot
Anti-OSBPL11 mouse monoclonal antibody (TA501938) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBPL11(RC209418).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (RC209418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11.
Anti-OSBPL11 mouse monoclonal antibody (TA501939) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBPL11(RC209418).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (RC209418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OSBPL11 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-OSBPL11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501940)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (RC209418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11.
HEK293T cells transfected with either RC209418 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-OSBPL11 antibody(TA501940), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (RC209418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OSBPL11 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (RC209418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11.
Anti-OSBPL11 mouse monoclonal antibody (TA502067) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBPL11(RC209418).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (RC209418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11.
Anti-OSBPL11 mouse monoclonal antibody (TA502068) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBPL11(RC209418).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (RC209418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OSBPL11 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-OSBPL11 antibody(TA502068),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-OSBPL11 antibody(TA502068),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
TA302906 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502068 | OSBPL11 antibody |
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| OSBPL11 mouse monoclonal antibody, clone OTI6A6 (formerly 6A6) | |||||||
 |
Mouse | IgG1 | OTI6A6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA302906 | OSBPL11 (C-term) antibody |
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| Goat Polyclonal Antibody against OSBPL11 | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA501938 | OSBPL11 antibody |
||||||
| OSBPL11 mouse monoclonal antibody, clone OTI2F6 (formerly 2F6) | |||||||
 |
Mouse | IgG1 | OTI2F6 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA501939 | OSBPL11 antibody |
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| OSBPL11 mouse monoclonal antibody, clone OTI6H9 (formerly 6H9) | |||||||
 |
Mouse | IgG2b | OTI6H9 | Purified | Can, Hu, Mky | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA501940 | OSBPL11 antibody |
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| OSBPL11 mouse monoclonal antibody, clone OTI5A10 (formerly 5A10) | |||||||
 |
Mouse | IgG1 | OTI5A10 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA501976 | OSBPL11 antibody |
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| OSBPL11 mouse monoclonal antibody, clone OTI7B3 (formerly 7B3) | |||||||
 |
Mouse | IgG2a | OTI7B3 | Purified | Can, Hu, Mky, Rt | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA501977 | OSBPL11 antibody |
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| OSBPL11 mouse monoclonal antibody, clone OTI2C8 (formerly 2C8) | |||||||
 |
Mouse | IgG1 | OTI2C8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA502067 | OSBPL11 antibody |
||||||
| OSBPL11 mouse monoclonal antibody, clone OTI10F6 (formerly 10F6) | |||||||
 |
Mouse | IgG1 | OTI10F6 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309418 | OSBPL11 |
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| Recombinant protein of human oxysterol binding protein-like 11 (OSBPL11) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411565 | OSBPL11 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |