PACSIN2 antibody
Principal name
PACSIN2 antibody
Alternative names for PACSIN2 antibody
Protein kinase C and casein kinase substrate in neurons protein 2
Ncbi ID
9606, NP_001171899.1, NM_007229, NP_009160, NP_001152981.1, NM_011862, NM_001159510, NM_130740, NM_001184971, NM_001184970
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PACSIN2 antibody
- Kit Component:
- - KN312743G1, Pacsin2 gRNA vector 1 in pCas-Guide vector
- KN312743G2, Pacsin2 gRNA vector 2 in pCas-Guide vector
- KN312743D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
IHC analysis of human cerebellum using PACSIN2 polyclonal antibody ( Cat # PAB0311 ) at 10 µg/ml.
Western blot analysis of PACSIN2 in human brain lysate in the 1 ) absence and 2 ) presence of immunizing peptide, 3 ) mouse brain lysate and 4 ) rat brain lysate. Using PACSIN2 polyclonal antibody ( Cat # PAB0311 ) at 0.05 µg/ml.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PACSIN2 polyclonal antibody ( Cat # PAB3301 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The PACSIN2 polyclonal antibody ( Cat # PAB3301 ) is used in Western blot to detect PACSIN2 in T-47D cell lysate ( Lane 1 ) and mouse brain tissue lysate ( Lane 2 ) .
Immunoperoxidase of monoclonal antibody to PACSIN2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Western Blot analysis of PACSIN2 expression in transfected 293T cell line (H00011252-T01) by PACSIN2 MaxPab polyclonal antibody.Lane 1: PACSIN2 transfected lysate(53.46 KDa).Lane 2: Non-transfected lysate.
Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE)
PACSIN2 Antibody (S357) (Cat.# TA325022) western blot analysis in Daudi,HL-60 cell line and mouse brain,rat heart lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).
PACSIN2 Antibody (S357) (Cat.# TA325022) western blot analysis in Daudi cell line and mouse brain lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Immunofluorescent analysis of Hela cells, using PACSIN2 Antibody (C-term) (Cat. #TA325022). TA325022 was diluted at 1:100 dilution. Alexa Fluor® 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (PACSIN2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (PACSIN2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA325022 | PACSIN2 (C-term) antibody |
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| Rabbit polyclonal PACSIN2 Antibody (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu, Ms | ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA349612 | PACSIN2 antibody |
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| Rabbit Polyclonal Anti-PACSIN2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP08269PU-N | PACSIN2 (400-450) antibody |
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 |
Rabbit | IgG | Aff - Purified | Bov, Can, Chk, Hu, Ms, Rt, Xen | P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300237 | PACSIN2 |
||||
| Recombinant protein of human protein kinase C and casein kinase substrate in neurons 2 (PACSIN2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416115 | PACSIN2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207795L2V | Lenti ORF particles, Pacsin2 (GFP-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,170.00
|
|
| OriGene Technologies, Inc. | ||
| MR222568L2V | Lenti ORF particles, Pacsin2 (GFP-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,170.00
|
|
| OriGene Technologies, Inc. | ||
| MR207795L1V | Lenti ORF particles, Pacsin2 (Myc-DDK-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,040.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201533L2V | Lenti ORF particles, Pacsin2 (GFP-tagged ORF) - Rat protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,170.00
|
|
| OriGene Technologies, Inc. | ||
| RR201533L1V | Lenti ORF particles, Pacsin2 (Myc-DDK-tagged ORF) - Rat protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,040.00
|
|
| OriGene Technologies, Inc. | ||