PACSIN3 antibody
Principal name
PACSIN3 antibody
Alternative names for PACSIN3 antibody
Endophilin I, Protein kinase C and casein kinase substrate in neurons protein 3, SH3 domain-containing protein 6511
Ncbi ID
9606, NP_001171903.1, NM_016223, NP_057307, NP_057307.2, NP_083009.1, NM_028733, NM_001184975, NM_001184974
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of PACSIN3 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
General readings
Cuajungco MP, Grimm C, Oshima K, D'hoedt D, Nilius B, Mensenkamp AR, Bindels RJ, Plomann M, Heller S.
PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4.
J Biol Chem. 2006 Jul 7;281(27):18753-62. Epub 2006 Apr 20.
PMID: 16627472
PACSIN3 polyclonal antibody (A01), Lot # 061101JCS1 Western Blot analysis of PACSIN3 expression in HeLa ( Cat # L013V1 ).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PACSIN3 polyclonal antibody ( Cat # PAB3303 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The PACSIN3 polyclonal antibody ( Cat # PAB3303 ) is used in Western blot to detect PACSIN3 in mouse brain tissue lysate.
PACSIN3 polyclonal antibody ( Cat # PAB6090 ) staining ( 1 µg/ml ) of NCI-H460 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
The anti-PACSIN3 Pab is used in Western blot to detect PACSIN3 in mouse brain tissue lysate.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PACSIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502212)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PACSIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502212)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502212), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502213), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502214), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502215), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502240), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PACSIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502249)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PACSIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502249)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502249), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502250), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502251), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502260), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502274), and then analyzed by flow cytometry.
TA302752 staining (1µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502212 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI2E4 (formerly 2E4) | |||||||
 |
Mouse | IgG1 | OTI2E4 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
||||||
| TA502249 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI4F8 (formerly 4F8) | |||||||
 |
Mouse | IgG1 | OTI4F8 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA302752 | PACSIN3 (N-term) antibody |
||||||
| Goat Polyclonal Antibody against PACSIN3 | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA502213 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI2A6 (formerly 2A6) | |||||||
 |
Mouse | IgG2a | OTI2A6 | Purified | Can, Hu, Mky, Rt | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA502214 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI4G7 (formerly 4G7) | |||||||
 |
Mouse | IgG2a | OTI4G7 | Purified | Can, Hu, Mky, Rt | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA502215 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI4A7 (formerly 4A7) | |||||||
 |
Mouse | IgG2b | OTI4A7 | Purified | Can, Hu, Mky | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA502240 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI4C5 (formerly 4C5) | |||||||
 |
Mouse | IgG1 | OTI4C5 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA502250 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI2D7 (formerly 2D7) | |||||||
 |
Mouse | IgG2b | OTI2D7 | Purified | Can, Hu, Mky, Rt | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA502251 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3) | |||||||
 |
Mouse | IgG1 | OTI2A3 | Purified | Can, Hu, Mky, Rt | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA502260 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1) | |||||||
 |
Mouse | IgG1 | OTI1E1 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA502274 | PACSIN3 antibody |
||||||
| PACSIN3 mouse monoclonal antibody, clone OTI4A5 (formerly 4A5) | |||||||
 |
Mouse | IgG1 | OTI4A5 | Purified | Can, Hu, Mky, Rt | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| AP15041PU-N | PACSIN3 (N-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304103 | PACSIN3 |
||||
| Recombinant protein of human protein kinase C and casein kinase substrate in neurons 3 (PACSIN3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414084 | PACSIN3 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206762L2V | Lenti ORF particles, Pacsin3 (GFP-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 3 (Pacsin3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206762L1V | Lenti ORF particles, Pacsin3 (Myc-DDK-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 3 (Pacsin3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
