PAFAH1B3 antibody
Principal name
PAFAH1B3 antibody
Alternative names for PAFAH1B3 antibody
PAFAHG, PAFAH subunit gamma, Platelet-activating factor acetylhydrolase IB subunit gamma, PAF acetylhydrolase 29 kDa subunit
Ncbi ID
9606, NP_002564, NP_001139411.1, NP_001139411, NP_001139412, NP_032802.1, NM_053654, NM_002573, NM_001145940
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of PAFAH1B3 antibody
- Kit Component:
- - KN312753G1, Pafah1b3 gRNA vector 1 in pCas-Guide vector
- KN312753G2, Pafah1b3 gRNA vector 2 in pCas-Guide vector
- KN312753D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Moro F, et al. (1998) Genomics 51 (1): 157–9TW, Li J, et al. (2001) Protein Eng. 14 (7): 513–9.
Immunofluorescence of monoclonal antibody to PAFAH1B3 on HeLa cell. [antibody concentration 10 ug/ml]
PAFAH1B3 monoclonal antibody (M02), clone 8C11. Western Blot analysis of PAFAH1B3 expression in PC-12 ( Cat # L012V1 ).
PAFAH1B3 monoclonal antibody (M02), clone 8C11. Western Blot analysis of PAFAH1B3 expression in Jurkat ( Cat # L017V1 ).
PAFAH1B3 monoclonal antibody (M02), clone 8C11 Western Blot analysis of PAFAH1B3 expression in IMR-32 ( Cat # L008V1 ).
Detection limit for recombinant GST tagged PAFAH1B3 is approximately 0.1ng/ml as a capture antibody.
PAFAH1B3 MaxPab polyclonal antibody. Western Blot analysis of PAFAH1B3 expression in human kidney.
Western Blot analysis of PAFAH1B3 expression in transfected 293T cell line (H00005050-T01) by PAFAH1B3 MaxPab polyclonal antibody.Lane 1: PAFAH1B3 transfected lysate(25.41 KDa).Lane 2: Non-transfected lysate.
PAFAH1B3 monoclonal antibody (M08), clone 3G6. Western Blot analysis of PAFAH1B3 expression in NIH/3T3 ( Cat # L018V1 ).
PAFAH1B3 monoclonal antibody (M08), clone 3G6. Western Blot analysis of PAFAH1B3 expression in PC-12 ( Cat # L012V1 ).
PAFAH1B3 monoclonal antibody (M08), clone 3G6. Western Blot analysis of PAFAH1B3 expression in Raw 264.7 ( Cat # L024V1 ).
PAFAH1B3 monoclonal antibody (M08), clone 3G6. Western Blot analysis of PAFAH1B3 expression in IMR-32 ( Cat # L008V1 ).
Detection limit for recombinant GST tagged PAFAH1B3 is approximately 3ng/ml as a capture antibody.
PAFAH1B3 MaxPab polyclonal antibody. Western Blot analysis of PAFAH1B3 expression in human kidney.
Western Blot analysis of PAFAH1B3 expression in transfected 293T cell line (H00005050-T01) by PAFAH1B3 MaxPab polyclonal antibody.Lane 1: PAFAH1B3 transfected lysate(25.41 KDa).Lane 2: Non-transfected lysate.
Human 293T; WB Suggested Anti-PAFAH1B3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate; PAFAH1B3 antibody - middle region (ARP56374_P050) in Human 293T cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAFAH1B3 (RC227227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAFAH1B3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAFAH1B3 (RC227227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAFAH1B3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAFAH1B3 (RC227227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAFAH1B3.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PAFAH1B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804470)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PAFAH1B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804470)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAFAH1B3 (RC227227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAFAH1B3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAFAH1B3 (RC227227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAFAH1B3.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PAFAH1B3 mouse monoclonal antibody. (TA804488) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PAFAH1B3 mouse monoclonal antibody. (TA804488) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PAFAH1B3 mouse monoclonal antibody. (TA804488) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PAFAH1B3 mouse monoclonal antibody. (TA804488) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PAFAH1B3 mouse monoclonal antibody. (TA804488) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAFAH1B3 mouse monoclonal antibody. (TA804488) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PAFAH1B3 mouse monoclonal antibody. (TA804488) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAFAH1B3 (RC227227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAFAH1B3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAFAH1B3 (RC227227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAFAH1B3.
Western blot analysis of SGC cells (Lane 1: 50ug lysate) and ECL cells (Lane 2: 70ug lysate) with anti-PAF Acetylhydrolase antibody diluated at 1:2000. (Predicted and observed size: 50kDa)
WB Suggested Anti-PAFAH1B3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 293T cell lysate
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA804488 | PAFAH1B3 antibody |
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| Purified PAFAH1B3 mouse monoclonal antibody, clone OTI5D2 (formerly 5D2) | |||||||
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Mouse | IgG1 | OTI5D2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
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| TA310074 | PAFAH1B3 antibody |
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| Rabbit anti-PAFAH1B3 polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€195.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA804356 | PAFAH1B3 antibody |
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| Purified PAFAH1B3 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) | |||||||
 |
Mouse | IgG1 | OTI2A5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804392 | PAFAH1B3 antibody |
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| PAFAH1B3 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3) | |||||||
 |
Mouse | IgG1 | OTI2D3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804457 | PAFAH1B3 antibody |
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| PAFAH1B3 mouse monoclonal antibody, clone OTI2E8 (formerly 2E8) | |||||||
 |
Mouse | IgG1 | OTI2E8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804470 | PAFAH1B3 antibody |
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| PAFAH1B3 mouse monoclonal antibody, clone OTI4A9 (formerly 4A9) | |||||||
 |
Mouse | IgG1 | OTI4A9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA804471 | PAFAH1B3 antibody |
||||||
| PAFAH1B3 mouse monoclonal antibody, clone OTI5B3 (formerly 5B3) | |||||||
 |
Mouse | IgG1 | OTI5B3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804553 | PAFAH1B3 antibody |
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| Purified PAFAH1B3 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2) | |||||||
 |
Mouse | IgG1 | OTI4B2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301268 | PAFAH1B3 (transcript variant 2) |
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| Recombinant protein of human platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa (PAFAH1B3), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP327227 | PAFAH1B3 (transcript variant 1) |
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| Recombinant protein of human platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa (PAFAH1B3), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP327237 | PAFAH1B3 (transcript variant 3) |
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| Recombinant protein of human platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa (PAFAH1B3), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR208741L2V | Lenti ORF particles, Pafah1b3 (GFP-tagged ORF) - Rat platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit (Pafah1b3), 200 uL, >10^7 TU/mL |
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 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR208741L1V | Lenti ORF particles, Pafah1b3 (Myc-DDK-tagged ORF) - Rat platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit (Pafah1b3), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||