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PAG1 / Csk-binding protein antibody

Principal name

PAG1 / Csk-binding protein antibody

Alternative names for PAG1 / Csk-binding protein antibody

PAG, CBP, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp

SwissProt ID

Q28755 (Sheep), Q29078 (Pig), Q29432 (Bovin), Q3U1F9 (Mouse), Q9JM80 (Rat), Q9NWQ8 (Human)

Gene ID

55824 (PAG1), 94212 (Pag1), 64019 (Cbp)

Ncbi ID

NP_060910.3, 9606, NP_001181960.1, NM_022253, NM_018440

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Background of PAG1 / Csk-binding protein antibody

CBP (also known as CREB-binding protein and CBP/p300) is a widely expressed nuclear protein that contains a bromodomain, a ZZ-type zinc domain, and TAZ-type zinc fingers. This 265 kD acetyltransferase enzyme, acetylates histones and non-histone proteins like NCOA3 coactivator and mediates c-AMP gene regulating by binding to phosphorylated CREB. CBP and phosphorylated CREB activates transcription of c-AMP responsive genes; defects in CBP result in Rubinstein-Taybi syndrome (mental retardation, craniofacial defects, heart defects). CBP is activated by TCR sigling and is involved in TNF-α gene expression and plays a central role in regulating gene responses to hypoxia. This protein is acetylated at multiple sites. CBP has been shown to interact with NCOA6, androgen receptor, STAT6, SRC1, Csk, FKHR, and c-jun and to form a complex with NCOA2, NCOA3, IKKA, IKKB, and IKBKG. In addition, CBP probably forms a complex with HIF1A and EP300. The Poly6064 antibody has been shown to be useful for Western blotting of the human and mouse CBP protein.

General readings

1. Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
2. Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604.
3. Brdickova N, Brdicka T, Andera L, Spicka J, Angelisova P, Milgram SL, Horejsi V: Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett. 2001 Oct 26;507(2):133-6.
Semac I, Palomba C, Kulangara K, Klages N, van Echten-Deckert G, Borisch B, Hoessli DC: Anti-CD20 therapeutic antibody rituximab modifies the functional organization of rafts/microdomains of B lymphoma cells. Cancer Res. 2003 Jan 15;63(2):534-40.
4. Davidson D, Bakinowski M, Thomas ML, Horejsi V, Veillette A: Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor. Mol Cell Biol. 2003 Mar;23(6):2017-28.
5. Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21. Epub 2005 Sep 13.
6. Svec A, Velenská Z, Horejsí V: Expression pattern of adaptor protein PAG: correlation between secondary lymphatic follicle and histogenetically related malignant lymphomas. Immunol Lett. 2005 Aug 15;100(1):94-7.
7. Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11.

Immunohistochemistry staining (paraffin-embedded sections) using anti-Cbp/PAG (MEM-255). Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.

SM3069P

Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human PAG. Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-PAG (PAG-C1) Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody)

SM3106P

Immunoprecipitation of human Cbp/PAG from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human Cbp/PAG (MEM-255; SM3069P). Note: Csk-binding protein (Cbp/PAG) is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-Cbp/PAG (PAG-C1). Lane 3: immunoprecipitation with anti-Cbp/PAG (polyclonal antibody; SP3003P).

SM3106P

Immunoprecipitation of human PAG1 from the lysate of Raji human Burkitt lymphoma cell line. Western blot was immunostained with human PAG1 monoclonal antibody, clone PAG - C1 ( Cat # MAB0934 ) . PAG1 is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1 , 2 : Immunoprecipitation with PAG1 monoclonal antibody, clone PAG - C1 ( Cat # MAB0934 ) . Lane 3 : Immunoprecipitation with PAG1 polyclonal antibody.

MAB0934

Immunohistochemistry staining ( paraffin - embedded sections ) using PAG1 monoclonal antibody, clone MEM - 255 ( Cat # MAB0935 ) . PAG1 is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma ( it is absent from mantle zone ) . PAG1 is also expressed more weakly in T cells in tonsil and the thymic medulla.

MAB0935

AP08997PU-N - PAG1 / Csk-binding protein

Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP08997PU-N

MCF-7 cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against CBP. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

TA328032

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA328032	PAG1 / Csk-binding protein antibody
	Rabbit Polyclonal anti-CBP Antibody
	Rabbit	IgG	Poly6064	Purified	Hu, Ms	WB	0.2 ml / €255.00
							OriGene Technologies, Inc.
AP08997PU-N	PAG1 / Csk-binding protein (97-432) antibody
	Rabbit			Aff - Purified	Hu, Ms	IP, P, WB	50 µg / €425.00
							OriGene Technologies GmbH
SM3069P	PAG1 / Csk-binding protein antibody
	Mouse	IgG2a	MEM-255	Purified	Hu	F, P, WB	0.1 mg / €260.00
							OriGene Technologies GmbH
SM3106P	PAG1 / Csk-binding protein antibody
	Mouse	IgG2b	PAG-C1	Purified	Bov, Hu, Ms, Rt	IP, P	0.1 mg / €260.00
							OriGene Technologies GmbH
+1 additional image
SP3003P	PAG1 / Csk-binding protein (97-432) antibody
	Rabbit			Purified	Hu, Ms	IP, P, WB	0.1 mg / €260.00
							OriGene Technologies GmbH

Rat Lenti ORF Particles

Catalog No.
RR210798L2V	Lenti ORF particles, Cbp (GFP-tagged ORF) - Rat Csk binding protein (Cbp), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR210798L1V	Lenti ORF particles, Cbp (Myc-DDK-tagged ORF) - Rat Csk binding protein (Cbp), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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