PAK1 antibody
Principal name
PAK1 antibody
Alternative names for PAK1 antibody
PAK 1, PAK-1, Alpha-PAK, PAK alpha, p21-activated kinase 1, p65-PAK, Serine/threonine-protein kinase PAK 1
SwissProt ID
O88643 (Mouse), P35465 (Rat), P38990 (Yeast), Q08E52 (Bovin), Q13153 (Human), Q17850 (Caeel)
Ncbi ID
NP_001122092.1, NP_001122092, 9606, NP_058894, 10116, NM_002576, NP_002567.3, NM_001128620, NM_017198, NP_058894.1
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), ELISA (detection) (E(detection)), Frozen Sections (C)
Background of PAK1 antibody
A brain serine threonine protein kinase is a target for p21 ras related proteins Cdc42 and Rac1. p21-activated kinases (PAKs) bind to and are activated by Rho family GTPases such as Cdc42 and Rac. These GTPases play key roles in regulating cell polarity, stress responses, and cell cycle progression (1). Stiochiometeric analyses using purified Rac and PAK suggest that Rac binds to PAK with high affinity in a 1:1 ratio. The kinase complexes specifically with aGTP bound activated p21, inhibiting the p21 activity and leading to activation and auto-phosphorylation of p65pak (2). Three isoforms of p21-activated serine/threonine kinases, PAK68, PAK65 and PAK62, were found in both cytosolic & membrane fraction, & their contents were significantly increased during induced retinoic acid differentiation of human promyelocytic leukemia HL60 cell (3). The content of PAK65 or Rac is not evident in plasma membrane fraction of cells form un-differentiated cells (2).
Constitutively activated Pak1 also suppress the activity of scavenger receptor class B, type 1 (SB-B1) in macrophage cell line RAW264.7, an action similar to LPS (4). Mutational studies with PAK1 also suggest that SR-B1 receptor down regulation involves binding to Rac and Cdc42. In mammalian cells, hyper osmotic shocks can induce apoptotic cell death. It is suggested that hyper osmotic shock can induce cleavage for PAK2 to produce a 36kDa kinase, which is the result of DNA fragmentation and activation of ICE/CED-3 family cysteine proteases termed caspases-3.
General readings
1、 Brown JL, Stowers L, Baer M, Trejo J, Coughlin S, Chant J (Mar 1997). "Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway". Curr Biol 6 (5): 598–605.
2、 Bekri S, Adelaide J, Merscher S, Grosgeorge J, Caroli-Bosc F, Perucca-Lostanlen D, Kelley PM, Pebusque MJ, Theillet C, Birnbaum D, Gaudray P (Apr 1998). "Detailed map of a region commonly amplified at 11q13-->q14 in human breast carcinoma". Cytogenet Cell Genet 79 (1–2): 125–31.
3、"Entrez Gene: PAK1 p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)".
4、 Bekri, S., Adelaide, J., Merscher, S., Grosgeorge, J., Caroli-Bosc, F., Perucca-Lostanlen, D., Kelley, P. M., Pebusque, M.-J., Theillet, C., Birnbaum, D., Gaudray, P. Detailed map of a region commonly amplified at 11q13-q14 in human breast carcinoma. Cytogenet. Cell Genet. 79: 125-131, 1997.
Immunoperoxidase of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged PAK1 is approximately 0.03ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to PAK1 on HeLa cell. [antibody concentration 35 ug/ml]
PAK1 monoclonal antibody (M02), clone 4D1 Western Blot analysis of PAK1 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged PAK1 is approximately 0.1ng/ml as a capture antibody.
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1 (phospho-Thr212) antibody (AP02438PU).
Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using PAK1 (phospho-Thr212) antibody (#AP02438PU, Red).
Figure 3. Western blot analysis of extracts from 293 cells untreated or treated with forskolin using PAK1 antibody (#AP02681PU)
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) antibody AP02447PU.
Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) antibody (#AP02447PU, Red).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1 antibody AP02681PU.
Immunofluorescence staining of methanol-fixed HeLa cells using PAK1 antibody AP02681PU (Red).
Western Blot analysis of extracts from 293 and 3T3 cells using PAK1 antibody
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1/PAK2/PAK3 antibody AP02688PU.
Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using PAK1/PAK2/PAK3 antibody AP02688PU (Red).
Immunohistochemistry (IHC) analyzes of PAK1 antibody (Cat.-No: AP06555PU-N) in paraffin-embedded human colon carcinoma tissue.
Western blot (WB) analysis of PAK1 antibody (Cat.-No.: AP06555PU-N) in extracts from 293 cells treated with Etoposide 25uM 60'.
Immunohistochemistry (IHC) analyzes of PAK1/2/3 antibody (Cat.-No.: AP06556PU-N) in paraffin-embedded human brain tissue.
Dot blot analysis of PAK1 (phospho T212) polyclonal antibody ( Cat # PAB0569 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 polyclonal antibody ( Cat # PAB2110 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of PAK1 ( arrow ) using PAK1 polyclonal antibody ( Cat # PAB2110 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PAK1 gene ( Lane 2 ) ( Origene Technologies ) .
PAK1 polyclonal antibody ( Cat # PAB7082 ) ( 1 µg/ml ) staining of rat brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of PAK1 expression in transfected 293T cell line (H00005058-T01) by PAK1 MaxPab polyclonal antibody.Lane1:PAK1 transfected lysate(49.17 KDa).Lane2:Non-transfected lysate.
Dot blot analysis of Phospho-PAK1-T212 Pab (Cat#AP12780PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Dot blot analysis of anti-PAK1-pThr423 Phospho-specific Pab (Cat.#AP12836PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PAK1 (arrow) using rabbit polyclonal PAK1 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2)
AP15710PU alpha PAK antibody staining of Human Breast Carcinoma.
Immunohistochemical staining of Pak1 on formalin fixed, paraffin embedded human breast cancer with Pak1 polyclonal antibody.
Dot blot analysis of PAK1 (phospho T423) polyclonal antibody ( Cat # PAB8092 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
Western blot analysis of PAK1 expression in transfected 293 cells.
Figure 1. Western blot of rat Hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabeling of PAK is completely eliminated by treatment with lambda-Ptase.
Immunohistochemistry analyzes of PAK1/2/3 antibody (AP20324PU-N) in paraffin-embedded human brain tissue.
Immunohistochemistry (IHC) analyzes of p-PAK1/2 (pThr423/402) antibody (Cat.-No.: AP20899PU-N) in paraffin-embedded human brain tissue.
Immunohistochemistry (IHC) analyzes of PAK1/2 antibody (Cat.-No.: AP20990PU-N) in paraffin-embedded human brain tissue.
Western blot (WB)analyzes of PAK1/2 antibody (Cat.-No.: AP20990PU-N) in extracts from CV-1 cells.
Immunohistochemistry (IHC) analyzes of PAKalpha antibody (Cat.-No.: AP21056PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of PAKalpha antibody (Cat.-No.: AP21056PU-N) in extracts from K562 cells.
Immunohistochemistry (IHC) analyzes of PAKalpha antibody (Cat.-No.: AP21120PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of PAKalpha antibody (Cat.-No.: AP21120PU-N) in extracts from 3T3 cells.
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Sample (30 ug of whole cell lysate). A: A431. 7.5% SDS PAGE. PAK1 antibody diluted at 1/1000
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged PAK1 is approximately 0.03ng/ml as a capture antibody.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody AP55728PU-N (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from LOVO cells treated with starved using PAK1/2 (Phospho-Ser199) Antibody AP55728PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody AP55769PU-N (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 3T3 cells treated with UV using PAK1 (Phospho-Ser204) Antibody AP55769PU-N.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of PAK1/2/3 (pS144/141/139) expression in HepG2 (A); mouse brain (B) whole cell lysates.
Immunohistochemical analysis of PAK1/2/3 (pS144/141/139) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
TA303131 (1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 61 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using PAK1 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted band size: 61 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using PAK1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of PAK1 expression in Etoposide treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Figure from citation: Western blot analysis of PAK1 protein level by using anti-PAK1 antibody in PC-3 and DU145 cells after infection with miR-203. View Citation
Western blot analysis of extracts from NIH-3T3 cells, using PAK1/2/3 (Ab-423/402/421) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells (Lane 2), using PAK1/2/3 (Ab-423/402/421) Antibody. The lane on the left is treated with synthesized peptide.
Western blot analysis of extracts from 3T3 cells, treated with UV (15mins), using PAK1 (Ab-204) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from cos-7 cells (Lane 2), using PAK1 (Ab-204) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PAK1 (Ab-204) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from K562 cells, using PAK1/2 (Ab-199) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using PAK1/2 (Ab-199) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts of mouse kidney cell line, using PAK1 antibody.
Western blot analysis of extracts from LOVO cells, treated with starved (24hours), using PAK1/2 (Phospho-Ser199) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody.The picture on the right is treated with the synthesized peptide.
Predicted band size: 68 kDa . Positive control: 293 cells untreated or treated with forskolin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with forskolin lysate Lane2: 293 cells treated with forskolin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm; Cell membrane. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using PAK1 (Phospho-Thr212) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm; Cell membrane. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using PAK1 (Phospho-Thr212) antibody at dilution 1/100. (Original magnification:×200)
Lysates
| Catalog No. | ||
|---|---|---|
| LY426982 | PAK1 overexpression lysate |
|
 |
0.1 mg /
€315.00
|
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| OriGene Technologies, Inc. |