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pan Actin antibody

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Principal name

pan Actin antibody

Available reactivities

All Species, Bov (Bovine), Can (Canine), Carp, GP (Guinea Pig), Gt (Goat), Hst (Hamster), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Sh (Sheep), Ze (Zebrafish), Chk (Chicken), Pl (Plant), Pr (Protozoa), Xen (Xenopus), Dictyostelium, Physarum, Mam (Mammalia), Mky (Monkey), Fe (Feline), Macaque, Amph (Amphibian), Re (Reptiles), Dros (Drosophila), Ye (Yeast), Snake, Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of pan Actin antibody

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis.1-3 Four of them represent the differentiation markers of muscle tissues and two are found in nearly all cells. There are three a-actins (a-skeletal, a-cardiac and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues.4 The N-terminal region of actin appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin.5 The actin in cells of various species and tissues are very similar in their immunological and physical properties. As a consequence, it is difficult to produce antisera to this protein. The availability of monoclonal antibody to actin provides a specific and useful tool in studying actin structure and function and in probing binding sites of actin-binding proteins.

General readings

1. Lessard J. (1988) Two Monoclonal antibodies to actin:
One muscle reactive and one generally reactive. Cell
Motility and Cytoskeleton 10: 349-362.

SM5137 - pan Actin
Western blot detection of actin in MDCK cell extract using SM5137
GTX11003 - pan Actin
Immunohistochemical analysis of Agarose-embedded zebrafish embryo tail section , using Actin(GTX11003) antibody at 1:200 dilution.
GTX11003 - pan Actin
ICC/IF analysis of Rat2 cells using Actin antibody [AC-40] at a dilution of 1:250 (red) and DAPI (blue).
GTX11003 - pan Actin
WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK, and (9) MDCK lysates using Actin antibody [AC-40] at a dilution of 1:500.
GTX11003 - pan Actin
Tissue stained with conserved C-terminus specific Monoclonal Anti-Actin (GTX11003). Indirect immunofluorescence staining of human tongue frozen section with Monoclonal Anti-Actin, Clone No. AC-40 (GTX11003).
NB600-535 - pan Actin
Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Immunoprecipitation and Western Blot analaysis on pan Actin from Human Cells.
GTX27813 - pan Actin
Formalin fixed paraffin embedded human leiomyoma stained with Muscle Actin antibody (cat. # GTX27813).
NB120-11003 - pan Actin
immunofluorescence: Actin Antibody (AC-40)[NB120-11003]-conserved C-terminus specific Monoclonal staining of human tongue frozen section with Monoclonal Anti-Actin
AP06000PU-N - pan Actin
Immunohistochemistry (IHC) analyzes of Actin antibody (Cat.-No.. AP06000PU-N) in paraffin-embedded human skeleta 1 muscle tissue.
AP06000PU-N - pan Actin
Western blot (WB) analysis of Actin antibody (Cat.-No.: AP06000PU-N) at 1/500 dilution
Lane1:Hela cell lysate
Lane2:Raw264.7 cell lysate
Lane3:PC12 cell lysate treated with UV
AP06487PU-N - pan Actin
Immunohistochemistry (IHC) analyzes of Actin-pan (alpha/beta/gamma) antibody (Cat.-No.: AP06487PU-N) in paraffin-embedded human skeletal muscle tissue.
AP06487PU-N - pan Actin
Western blot (WB) analysis of Actin-alpha/gamma antibody (Cat.-No.: AP06487PU-N) in extracts from mouse heart.
NBP1-41294 - pan Actin
Immunohistochemistry-Frozen: Actin Antibody (AC-40) [NBP1-41294]
NBP1-41294 - pan Actin
Western Blot: Actin Antibody (AC-40) [NBP1-41294]
AP23240PU-N - pan Actin
Actin Polyclonal Antibody
AP23240PU-N - pan Actin
Actin Polyclonal Antibody
AM26032PU-N - pan Actin
Western blotting analysis of Muscle-Specific Actin using the monoclonal antibody Cat.-No. AM26032PU-N (Clone HHF35). Lane 1: Murine femoral muscle. Lane 2: Murine heart. Lane 3: HeLa. Lane 4: HUVEC.
AP23463PU-N - pan Actin
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23463PU-N - pan Actin
Western blot analysis of extracts from HeLa cells, using pan Actin Antibody. The lane on the right is treated with the synthesized peptide.
DB 001-IHC-1 - pan Actin
Formalin-fixed and paraffin-embedded human stomach tissue(4 μm)stained with anti-actin (DB 001)antibody, shows strong positive immunostaining of smooth muscle cells.
DB 001-IHC-0.5 - pan Actin
Formalin-fixed and paraffin-embedded human stomach tissue(4 μm)stained with anti-actin (DB 001)antibody, shows strong positive immunostaining of smooth muscle cells.
DB 001-0.1 - pan Actin
Anti-actin (DB 001). Western blot of actin in mouse brain crude lysate (50μg of protein loaded).
DB 001-0.05 - pan Actin
Anti-actin (DB 001). Western blot of actin in mouse brain crude lysate (50μg of protein loaded).
DB 001-IHC-0.2 - pan Actin
Formalin-fixed and paraffin-embedded human stomach tissue(4 μm)stained with anti-actin (DB 001)antibody, shows strong positive immunostaining of smooth muscle cells.
DB 001-IHC-0.1 - pan Actin
Formalin-fixed and paraffin-embedded human stomach tissue(4 μm)stained with anti-actin (DB 001)antibody, shows strong positive immunostaining of smooth muscle cells.
AM26032SU-N - pan Actin
Formalin-Fixed, Paraffin-Embedded Human uterus stained with Actin antibody Cat.-No. AM26032SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of smooth muscle fibers.

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

pan Actin antibody
pan Actin Mouse IgG2a AC-40 Aff - Purified Chk, Hu, Ms, Rt, Snake C, ICC/IF, WB
0.1 mg / €390.00
  OriGene Technologies GmbH

pan Actin (pan alpha / gamma 2) antibody
Western blotting analysis of Muscle-Specific Actin using the monoclonal antibody Cat.-No. AM26032PU-N (Clone HHF35). Lane 1: Murine femoral muscle. Lane 2: Murine heart. Lane 3: HeLa. Lane 4: HUVEC. Mouse IgG1 HHF35 Aff - Purified Can, Chk, Fe, Hu, Ms, Prim, Rb, Rt C, P, WB
0.1 mg / €260.00
  OriGene Technologies GmbH

pan Actin (pan alpha / gamma 2) antibody
Formalin-Fixed, Paraffin-Embedded Human uterus stained with Actin antibody Cat.-No. AM26032SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of smooth muscle fibers. Mouse IgG1 HHF35 Supernatant Can, Chk, Fe, Hu, Ms, Prim, Rb, Rt C, P, WB
1 ml / €400.00
  OriGene Technologies GmbH

pan Actin antibody
Immunohistochemistry (IHC) analyzes of Actin antibody (Cat.-No.. AP06000PU-N) in paraffin-embedded human skeleta 1 muscle tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

pan Actin antibody
Immunohistochemistry (IHC) analyzes of Actin-pan (alpha/beta/gamma) antibody (Cat.-No.: AP06487PU-N) in paraffin-embedded human skeletal muscle tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

pan Actin (367-377) antibody
Actin Polyclonal Antibody Rabbit IgG Aff - Purified Hu, Rt P, WB
0.1 mg / €390.00
  OriGene Technologies GmbH
+1 additional image

pan Actin (C-term) antibody
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µl / €425.00
  OriGene Technologies GmbH
+1 additional image

7 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (7)
Clonality
  1. Polyclonal (4)
  2. Monoclonal (3)
Target
  1. pan Actin (7)
Property
  1. 367-377 (1)
  2. C-term (1)
  3. pan alpha / gamma 2 (2)
Reactivity
  1. Human (Hu) (7)
  2. Rat (Rt) (7)
  3. Mouse (Ms) (6)
  4. Chicken (Chk) (3)
  5. Canine (Can) (2)
  6. Feline (Fe) (2)
  7. Primate (Prim) (2)
  8. Rabbit (Rb) (2)
  9. Snake (1)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (6)
  3. Frozen sections (C) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Enzyme Immunoassay (E) (1)
Presentation
  1. Aff - Purified (6)
  2. Supernatant (1)
Host
  1. Rabbit (4)
  2. Mouse (3)
Images
  1. 1 image (6)
Images: Applications
  1. Paraffin Sections (P) (5)
  2. Western blot / Immunoblot (WB) (5)
Manufacturer
  1. OriGene Technologies (7)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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