PARG antibody
Principal name
PARG antibody
Alternative names for PARG antibody
PARG99, Poly(ADP-ribose) glycohydrolase
Ncbi ID
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of PARG antibody
- Kit Component:
- - KN208530G1, PARG gRNA vector 1 in pCas-Guide vector
- KN208530G2, PARG gRNA vector 2 in pCas-Guide vector
- KN208530D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Whatcott, C.J., et al. Exp. Cell Res. 315(20):3477-3485(2009)
Frizzell, K.M., et al. J. Biol. Chem. 284(49):33926-33938(2009)
Erdelyi, K., et al. FASEB J. 23(10):3553-3563(2009)
Ame, J.C., et al. J. Cell. Sci. 122 (PT 12), 1990-2002 (2009)
Uchiumi, F., et al. Genes Cells 13(12):1229-1247(2008)
Immunofluorescence of purified MaxPab antibody to PARG on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of PARG expression in transfected 293T cell line (H00008505-T01) by PARG MaxPab polyclonal antibody.Lane 1: PARG transfected lysate(107.36 KDa).Lane 2: Non-transfected lysate.
PARG MaxPab polyclonal antibody. Western Blot analysis of PARG expression in Hela NE.
Western blot analysis of PARG Antibody (C-term) Cat.-No AP53163PU-N in NCI-H292 cell line lysates (35ug/lane). This demonstrates the PARG antibody detected the PARG protein (arrow).
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PARG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808508)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARG (RC208530, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARG.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARG (RC208530, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARG.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARG (RC208530, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARG.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808614)(1:150)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808614)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808614)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PARG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808614)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PARG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808614)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PARG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808614)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PARG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808614)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARG (RC208530, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARG.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARG (RC208530, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARG.(1:2000)
Western blot analysis of Parg (arrow) using rabbit polyclonal Parg Antibody (C-term) (Cat.#TA302286).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Parg gene (Lane 2) (Origene Technologies).
Western blot analysis of Parg Antibody (C-term) in 293 cell line lysates (35ug/lane). Parg (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Parg antibody (C-term) (Cat.#TA302286), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808614 | PARG antibody |
||||||
| PARG mouse monoclonal antibody, clone OTI6F4 (formerly 6F4) | |||||||
 |
Mouse | IgG2a | OTI6F4 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
|
||||||
| TA302286 | PARG (C-term) antibody |
||||||
| Rabbit Polyclonal Antibody against PARG (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA808508 | PARG antibody |
||||||
| PARG mouse monoclonal antibody, clone OTI1B5 (formerly 1B5) | |||||||
 |
Mouse | IgG1 | OTI1B5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA808515 | PARG antibody |
||||||
| PARG mouse monoclonal antibody, clone OTI7F4 (formerly 7F4) | |||||||
 |
Mouse | IgG2b | OTI7F4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA808567 | PARG antibody |
||||||
| PARG mouse monoclonal antibody, clone OTI12G3 (formerly 12G3) | |||||||
 |
Mouse | IgG2b | OTI12G3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA808712 | PARG antibody |
||||||
| PARG mouse monoclonal antibody, clone OTI9F6 (formerly 9F6) | |||||||
 |
Mouse | IgG1 | OTI9F6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| AP53163PU-N | PARG (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR202387L2V | Lenti ORF particles, Parg (GFP-tagged ORF) - Rat poly (ADP-ribose) glycohydrolase (Parg), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,850.00
|
|
| OriGene Technologies, Inc. | ||
| RR202387L1V | Lenti ORF particles, Parg (Myc-DDK-tagged ORF) - Rat poly (ADP-ribose) glycohydrolase (Parg), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,660.00
|
|
| OriGene Technologies, Inc. | ||