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PARP16 antibody

Principal name

PARP16 antibody

Alternative names for PARP16 antibody

PARP-16, C15orf30, Poly [ADP-ribose] polymerase 16

SwissProt ID

Q5U2Q4 (Rat), Q7TMM8 (Mouse), Q8N5Y8 (Human), Q8N5Y8-3

Gene ID

54956 (PARP16), 214424 (Parp16), 315760

Ncbi ID

NP_060321, 9606, NP_060321.3, NM_017851, NP_803411.3, NM_001014093

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of PARP16 antibody

Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. PARP16 is a member of poly(ADP-ribose) polymerases (PARPs) family that is encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.

General readings

Strausberg,R.L., et al., (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903

Human kidney; WB Suggested Anti-PARP16 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human kidney; PARP16 antibody - N-terminal region (ARP33751_P050) in Human kidney cells using Western Blot

ARP33751_P050

Human 293T; Host: Rabbit . Target Name: PARP16 . Sample Tissue: 293T . Antibody Dilution: 1.0ug/ml. PARP16 is supported by BioGPS gene expression data to be expressed in HEK293T.; PARP16 antibody - middle region (ARP33752_P050) in Human 293T cells using Western Blot

ARP33752_P050

Human 293T; WB Suggested Anti-PARP16 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. PARP16 is supported by BioGPS gene expression data to be expressed in HEK293T.; PARP16 antibody - middle region (ARP33752_P050) in Human 293T cells using Western Blot

ARP33752_P050

HEK293T cells transfected with either RC202846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PARP16 antibody(TA500452), and then analyzed by flow cytometry.

TA500452

HEK293T cells transfected with either RC202846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PARP16 antibody(TA500453), and then analyzed by flow cytometry.

TA500453

TP302846

WB Suggested Anti-PARP16 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human kidney

TA334256

WB Suggested Anti-PARP16 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysatePARP16 is supported by BioGPS gene expression data to be expressed in HEK293T

TA334257

Host: Rabbit; Target Name: PARP16; Sample Tissue: 293T; Antibody Dilution: 1.0 ug/mlPARP16 is supported by BioGPS gene expression data to be expressed in HEK293T

TA334257

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA500452	PARP16 antibody
	anti-PARP16 mouse monoclonal antibody, clone OTI2C8 (formerly 2C8)
	Mouse	IgG1	OTI2C8	Purified	Hu	F	0.1 ml / €379.00
							OriGene Technologies, Inc.

TA500453	PARP16 antibody
	anti-PARP16 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2)
	Mouse	IgG1	OTI4E2	Purified	Hu	F	0.1 ml / €360.00
							OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP302846	PARP16
	Recombinant protein of human poly (ADP-ribose) polymerase family, member 16 (PARP16)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY402632	PARP16 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR203347L1V	Lenti ORF particles, Parp16 (Myc-DDK-tagged ORF) - Rat poly (ADP-ribose) polymerase family, member 16 (Parp16), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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