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PAX6 antibody

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Principal name

PAX6 antibody

Alternative names for PAX6 antibody

Pax-6, Aniridia type II protein, AN2, Oculorhombin, Paired box protein Pax-6

SwissProt ID

O18381 (Drome), P26367 (Human), P26630 (Danre), P47237 (Chick), P55864 (Xenla), P63015 (Mouse), P63016 (Rat), Q1LZF1 (Bovin)

Gene ID

5080 (PAX6), 18508 (Pax6), 25509

Ncbi ID

NP_001595, 9606, NP_000271, NP_000271.1, NP_038655.1, NM_013627, NM_013001, NM_000280, NM_001604

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C)

Background of PAX6 antibody

Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells .PAX6 is the most researched of the PAX genes and appears throughout the literature as a "master control" gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. This transcription factor is most famous for its use in the interspecifically induced expression of ectopic eyes and is of medical importance because heterozygous mutants produce a wide spectrum of ocular defects such as Aniridia in humans. This gene encodes paired box gene 6, one of many human homologues of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause aniridia as well as Peter's anomaly, both ocular diseases.

General readings

Kim,E.A., et al., (2006) J. Biol. Chem. 281 (11), 7489-7497

ARP38062_P050 - PAX6
Human HepG2; WB Suggested Anti-PAX6 Antibody Titration: 0.0625ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; PAX6 antibody - N-terminal region (ARP38062_P050) in Human HepG2 cells using Western Blot
PAB7271 - PAX6
PAX6 polyclonal antibody ( Cat # PAB7271 ) ( 0.5 µg/ml ) staining of mouse eye lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
ARP32741_P050 - PAX6
Human Jurkat; WB Suggested Anti-PAX6 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; PAX6 antibody - N-terminal region (ARP32741_P050) in Human Jurkat cells using Western Blot
H00005080-B01P - PAX6
PAX6 MaxPab polyclonal antibody. Western Blot analysis of PAX6 expression in human pancreas.
H00005080-B01P - PAX6
Western Blot analysis of PAX6 expression in transfected 293T cell line (H00005080-T01) by PAX6 MaxPab polyclonal antibody.Lane 1: PAX6 transfected lysate(46.42 KDa).Lane 2: Non-transfected lysate.
H00005080-D01P - PAX6
PAX6 MaxPab rabbit polyclonal antibody. Western Blot analysis of PAX6 expression in mouse spleen.
H00005080-D01P - PAX6
PAX6 MaxPab rabbit polyclonal antibody. Western Blot analysis of PAX6 expression in human kidney.
H00005080-D01P - PAX6
Western Blot analysis of PAX6 expression in transfected 293T cell line (H00005080-T02) by PAX6 MaxPab polyclonal antibody.Lane 1: PAX6 transfected lysate(46.70 KDa).Lane 2: Non-transfected lysate.
AP17622PU-N - PAX6
Western blot analysis of PAX6 Antibody (Center) (AP17622PU-N) in Hela cell line lysates (35ug/lane). PAX6 (arrow) was detected using the purified Pab.
H00005080-D01 - PAX6
PAX6 MaxPab rabbit polyclonal antibody. Western Blot analysis of PAX6 expression in human kidney.
H00005080-D01 - PAX6
PAX6 MaxPab rabbit polyclonal antibody. Western Blot analysis of PAX6 expression in mouse spleen.
H00005080-D01 - PAX6
Immunoprecipitation of PAX6 transfected lysate using anti-PAX6 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PAX6 MaxPab mouse polyclonal antibody (B01) (H00005080-B01).
AP53180PU-N - PAX6
PAX6 Antibody (Cat. #AP53180PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAX6 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
AP53180PU-N - PAX6
Confocal immunofluorescent analysis of PAX6 Antibody(Cat#AP53180PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
AP53180PU-N - PAX6
Western blot analysis of PAX6 (arrow) using rabbit polyclonal PAX6 Antibody (Cat. #AP53180PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.
AM06472SU-N - PAX6
Western blot analysis using PAX6 mAb against human PAX6 (AA: 1-223) recombinant protein. (Expected MW is 50 kDa)
AM06472SU-N - PAX6
Flow cytometric analysis of 3T3-L1 cells using PAX6 mouse mAb (green) and negative control (purple).
AM50305PU-S - PAX6
Western blot analysis of PAX6 in human cerebellum lysate using PAX6 Antibody Cat.-No AM50305PU-N (Clone PAX6/498).
TP760453 - PAX6
TA303232 - PAX6
TA303232 (0.5µg/ml) staining of Mouse Eye lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA318938 - PAX6
Western blot analysis of PAX6 with rat kidney tissue lysate.
TA331135 - PAX6
WB Suggested Anti-PAX6 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
TA335202 - PAX6
WB Suggested Anti-PAX6 Antibody Titration: 0.0625 ug/ml; ELISA Titer: 1: 12500; Positive Control: HepG2 cell lysate

11 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAX6 antibody
  Goat Polyclonal Antibody against PAX6  
TA303232 (0.5µg/ml) staining of Mouse Eye lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

PAX6 antibody
  Rabbit polyclonal anti-PAX6 antibody  
Western blot analysis of PAX6 with rat kidney tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €355.00
  OriGene Technologies, Inc.

PAX6 antibody
Western blot analysis using PAX6 mAb against human PAX6 (AA: 1-223) recombinant protein. (Expected MW is 50 kDa) Mouse IgG1 1C8 Ascites Hu E, F, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+1 additional image

PAX6 antibody
Western blot analysis of PAX6 in human cerebellum lysate using PAX6 Antibody Cat.-No AM50305PU-N (Clone PAX6/498). Mouse IgG1 PAX6/496 Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, WB
0.1 mg / €430.00
  OriGene Technologies GmbH

PAX6 antibody
Western blot analysis of PAX6 in human cerebellum lysate using PAX6 Antibody Cat.-No AM50305PU-N (Clone PAX6/498). Mouse IgG1 PAX6/496 Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, WB
20 µg / €240.00
  OriGene Technologies GmbH

PAX6 (Center) antibody
Western blot analysis of PAX6 Antibody (Center) (AP17622PU-N) in Hela cell line lysates (35ug/lane). PAX6 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

PAX6 antibody
PAX6 Antibody (Cat. #AP53180PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAX6 Antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, P ICC/IF, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+2 additional images

PAX6 antibody
PAX6 Rabbit Purified Hu, Ms, Rt, Xen, Ze E, WB
0.1 mg / €450.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PAX6 (full length, N-term HIS tag, transcript variant 2)
  Purified recombinant protein of Human paired box 6 (PAX6), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
PAX6 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pax6 (GFP-tagged ORF) - Rat paired box 6 (Pax6), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pax6 (GFP-tagged ORF) - Rat paired box 6 (Pax6), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Pax6 (Myc-DDK-tagged ORF) - Rat paired box 6 (Pax6), 200 uL, >10^7 TU/mL
Lenti ORF particles, Pax6 (Myc-DDK-tagged ORF) - Rat paired box 6 (Pax6), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

11 Item(s)

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11 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (8)
  2. Protein | Growth Factor (1)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (5)
  2. Monoclonal (3)
Target
  1. PAX6 (9)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (8)
  2. Mouse (Ms) (5)
  3. Rat (Rt) (5)
  4. Chicken (Chk) (2)
  5. P (1)
  6. Xenopus (Xen) (1)
  7. Zebrafish (Ze) (1)
Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Flow cytometry (F) (3)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  4. Frozen sections (C) (2)
  5. Enzyme Immunoassay (E) (2)
  6. Immunoprecipitation (IP) (2)
Presentation
  1. Aff - Purified (3)
  2. Purified (3)
  3. Ascites (1)
Host
  1. Rabbit (4)
  2. Mouse (3)
  3. Goat (1)
Images
  1. 1 image (8)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Flow cytometry (F) (1)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. E. coli (1)
Manufacturer
  1. OriGene Technologies (11)
Service
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  • Germany
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