PAX7 antibody
Principal name
PAX7 antibody
Alternative names for PAX7 antibody
Pax-7, HUP1, Paired box protein Pax-7
Ncbi ID
NP_002575, NP_001128726.1, 9606, NP_001128726, NM_013945, NM_002584, NM_001135254, NP_035169.1, NM_011039
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Flow Cytometry (F), Immunoprecipitation (IP)
Background of PAX7 antibody
PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box gene 7 is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown. This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.
General readings
Moller,E., (2007) Cancer Genet. Cytogenet. 178 (2), 114-119
Human kidney; Rabbit Anti-PAX7 Antibody. Catalog Number: ARP32393. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; PAX7 antibody - N-terminal region (ARP32393_P050) in Human kidney cells using Immunohistochemistry
Human Skin; Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-PAX7 antibody (ARP32393_P050); PAX7 antibody - N-terminal region (ARP32393_P050) in Human Skin cells using Immunohistochemistry
Jurkat; Host: Rabbit. Target Name:PAX7. Sample Tissue:Jurkat. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:0.0625ug/mL. Peptide Concentration: 8.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%.; PAX7 antibody - N-terminal region (ARP32393_P050) in Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-PAX7 Antibody Titration: 0.05ug/ml. Positive Control: Jurkat cell lysate; PAX7 antibody - N-terminal region (ARP32393_P050) in Human Jurkat cells using Western Blot
Mouse skeletal muscle; . Lanes: 1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate, 3: Mouse skeletal muscle lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:10000. Gene Name: PAX7. Submitted by: Anonymous.; PAX7 antibody - N-terminal region (ARP32393_P050) in Mouse skeletal muscle cells using Western Blot
PAX7 monoclonal antibody (M03), clone 3C9. Western Blot analysis of PAX7 expression in Raw 264.7 ( Cat # L024V1 ).
PAX7 monoclonal antibody (M03), clone 3C9. Western Blot analysis of PAX7 expression in PC-12 ( Cat # L012V1 ).
PAX7 monoclonal antibody (M03), clone 3C9 Western Blot analysis of PAX7 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged PAX7 is approximately 3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to PAX7 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 0.5 ug/ml]
PAX7 monoclonal antibody (M05), clone 1E12 Western Blot analysis of PAX7 expression in NIH/3T3 ( Cat # L018V1 ).
Detection limit for recombinant GST tagged PAX7 is approximately 3ng/ml as a capture antibody.
PAX7 monoclonal antibody (M06), clone 3H1 Western Blot analysis of PAX7 expression in HeLa ( Cat # L013V1 ).
PAX7 monoclonal antibody (M06), clone 3H1. Western Blot analysis of PAX7 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged PAX7 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PAX7 is approximately 0.3ng/ml as a capture antibody.
Human Fetal heart; Host: Rabbit. Target Name:PAX7. Sample Tissue:Fetal heart lysates. Antibody Dilution: 1.0ug/ml.; PAX7 Antibody - C-terminal region (ARP32742_P050) in Human Fetal heart cells using Western Blot
Immunofluorescence of monoclonal antibody to PAX7 on HeLa cell. [antibody concentration 10 ug/ml]
PAX7 monoclonal antibody (M07), clone 1G11 Western Blot analysis of PAX7 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged PAX7 is approximately 1ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-PAX7 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; PAX7 antibody - N-terminal region (ARP32392_P050) in Human HepG2 cells using Western Blot
Hum. Fetal Muscle; Host: Rabbit. Target Name: NOP56 . Sample Tissue: Human Fetal Muscle . Antibody Dilution: 1.0ug/ml.; PAX7 antibody - middle region (ARP32068_P050) in Hum. Fetal Muscle cells using Western Blot
Hum. Adult Placenta; Host: Rabbit. Target Name: SERPINA3 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; PAX7 antibody - middle region (ARP32068_P050) in Hum. Adult Placenta cells using Western Blot
Human HT1080; WB Suggested Anti-PAX7 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate; PAX7 antibody - middle region (ARP32068_P050) in Human HT1080 cells using Western Blot
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
PAX7 monoclonal antibody clone 1E12 Western Blot analysis of PAX7 expression in NIH/3T3.
Detection limit for recombinant GST tagged PAX7 is approximately 3 ng/ml as a capture antibody.
PAX7 Antibody (Center) (Cat. #AP53181PU-N) western blot analysis in CEM cell line lysates (35µg/lane).This demonstrates the PAX7 antibody detected the PAX7 protein (arrow).
PAX7 Antibody (C-term) (Cat. #AP53182PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAX7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
PAX7 Antibody (C-term) (Cat. #AP53182PU-N) western blot analysis in mouse spleen tissue lysates (35µg/lane).This demonstrates the PAX7 antibody detected the PAX7 protein (arrow).
Western blot analysis of PAX7 in human HeLa Cell lysate using PAX7 Antibody Cat.-No AM50306PU-N (Clone PAX7/497).
Anti-PAX7 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PAX7 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-PAX7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HT1080 cell lysate
2Hum. Adult Placenta; Host: Rabbit; Target Name: SERPINA3; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0ug/ml
3Hum. Fetal Muscle; Host: Rabbit; Target Name: NOP56; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0ug/ml
WB Suggested Anti-PAX7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate
Lanes:1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate, 3: Mouse skeletal muscle lysate; Primary Antibody Dilution:1:1000; Secondary Antibody:Anti-rabbit HRP; Secondary Antibody Dilution:1:10000; Gene Name:PAX7; Submitted by:Anonymous;
WB Suggested Anti-PAX7 Antibody Titration: 0.05ug/ml; Positive Control: Jurkat cell lysate
Host: Rabbit; Target Name:PAX7; Sample Tissue:Jurkat; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:0.0625ug/mL; Peptide Concentration: 8.0ug/mL; Lysate Quantity: 25ug/lane; Gel Concentration: 12%
Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-PAX7 antibody
Rabbit Anti-PAX7 Antibody; Paraffin Embedded Tissue: Human Kidney; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Host: Rabbit; Target Name:PAX7; Sample Tissue:Fetal heart lysates; Antibody Dilution: 1.0ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323861 | PAX7 antibody |
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| Anti-PAX7 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM20925PU-N | PAX7 (411-521) antibody |
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 |
Mouse | IgG2a | 1E12 | Purified | Hu, Ms | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
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| AM50306PU-S | PAX7 antibody |
||||||
 |
Mouse | IgG1 | PAX7/497 | Purified | Hu, Ms, Ze | C, F, ICC/IF, IP, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
| AM50306PU-T | PAX7 antibody |
||||||
|
Mouse | IgG1 | PAX7/497 | Purified | Hu, Ms, Ze | C, F, ICC/IF, IP, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
| AP53181PU-N | PAX7 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP53182PU-N | PAX7 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| MO15020-100 | PAX7 antibody |
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 |
Mouse | IgG1 | PAX7 | Purified | Chk, Hu, Ms, Rt | C, ICC/IF |
0.1 mg /
€340.00
|
| Neuromics Antibodies | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY415548 | PAX7 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY419235 | PAX7 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY427610 | PAX7 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY429110 | PAX7 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
