PAX8 antibody
Principal name
PAX8 antibody
Alternative names for PAX8 antibody
Pax-8, Paired box protein Pax-8
Ncbi ID
9606, NP_003457, NP_003457.1, NP_039247, NM_011040, NP_006149, NP_054698, AAH01060.1, NM_013992, NP_035170.1, NM_031141, NM_003466, NM_013952, NM_013953
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of PAX8 antibody
This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas.Tissue specificity: Expressed in the excretory system, thyroid gland and Wilms tumors.ABCAM: This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described. Pax8 is also a marker of otic progenitor cells.
General readings
Castro P, Rebocho AP, Soares RJ, Magalhaes J, Roque L, Trovisco V, Vieira de Castro I, Cardoso-de-Oliveira M, Fonseca E, Soares P, Sobrinho-Simoes M.
PAX8-PPAR{gamma} rearrangement is frequently detected in the follicular variant of papillary thyroid carcinoma.
J Clin Endocrinol Metab. 2005 Oct 11; [Epub ahead of print]
PMID: 16219715
PAX8 monoclonal antibody (M03), clone 3B11. Western Blot analysis of PAX8 expression in RIN-m5F. (60kD,Endocrinology Vol. 140, No. 10 4651-4658,http://endo.endojournals.org/cgi/content/full/140/10/4651)
Detection limit for recombinant GST tagged PAX8 is approximately 3ng/ml as a capture antibody.
Western Blot analysis of PAX8 expression in transfected 293T cell line by PAX8 monoclonal antibody (M04), clone 2D6.Lane 1: PAX8 transfected lysate(48.119 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged PAX8 is approximately 1ng/ml as a capture antibody.
Western Blot analysis of PAX8 expression in transfected 293T cell line by PAX8 monoclonal antibody (M05), clone 3E9.Lane 1: PAX8 transfected lysate(48.2 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged PAX8 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PAX8 is approximately 1ng/ml as a capture antibody.
Western Blot analysis of PAX8 expression in transfected 293T cell line by PAX8 monoclonal antibody (M07), clone 1A6.Lane 1: PAX8 transfected lysate(48.119 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged PAX8 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PAX8 is approximately 1ng/ml as a capture antibody.
PAX8 monoclonal antibody (M09), clone 3H7. Western Blot analysis of PAX8 expression in human liver.
PAX8 monoclonal antibody (M09), clone 3H7. Western Blot analysis of PAX8 expression in Hs 181.Tes.
PAX8 monoclonal antibody (M09), clone 3H7 Western Blot analysis of PAX8 expression in LNCaP ( Cat # L004V1 ).
Detection limit for recombinant GST tagged PAX8 is approximately 3ng/ml as a capture antibody.
PAX8 monoclonal antibody (M10), clone 3G11. Western Blot analysis of PAX8 expression in human ovary cancer.
PAX8 monoclonal antibody (M10), clone 3G11. Western Blot analysis of PAX8 expression in human parotid gland.
Detection limit for recombinant GST tagged PAX8 is approximately 1ng/ml as a capture antibody.
Immunohistochemical staining of Formalin-fixed, Paraffin embedded human kidney tissue (cortex) using anti-PAX8 antibody cat.no. AP07520PU-N
PAX8 polyclonal antibody ( Cat # PAB6201 ) staining ( 3 µg/ml ) of paraffin embedded human thyroid gland. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
PAX8 polyclonal antibody ( Cat # PAB6919 ) ( 0.5 µg/ml ) staining of 293 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Human HepG2; WB Suggested Anti-PAX8 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; PAX8 antibody - N-terminal region (ARP48088_P050) in Human HepG2 cells using Western Blot
Staining of paraffin embedded Human Thyroid Gland using PAX8 Antibody Cat.-No AP16042PU-N at 4 µg/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Western Blot analysis of PAX8 expression in transfected 293T cell line by PAX8 monoclonal antibody (M23), clone 2F1.Lane 1: PAX8 transfected lysate(48.2 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of PAX8 Antibody (Center) (AP17623PU-N) in HL-60 cell line lysates (35ug/lane). PAX8 (arrow) was detected using the purified Pab.
Human Thyroid; Thyroid; PAX8 antibody - middle region (ARP34685_P050) in Human Thyroid cells using Immunohistochemistry
Human NCI-H226; WB Suggested Anti-PAX8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: NCI-H226 cell lysate; PAX8 antibody - middle region (ARP34685_P050) in Human NCI-H226 cells using Western Blot
Immunohistochemistry (IHC) analyzes of Pax-8 antibody (Cat.-No.: AP20584PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of Pax-8 antibody (Cat.-No.: AP20584PU-N) in extracts from HT-29 cells.
Mouse Pancreas; WB Suggested Anti-Pax8 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Pancreas; Pax8 antibody - C-terminal region (ARP34180_P050) in Mouse Pancreas cells using Western Blot
Formalin-Fixed, Paraffin-Embedded endometrial clear cell carcinoma stained with PAX8 antibody Cat.-No. AM10131SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of tumor cells.
Western blot analysis using PAX8 antibody Cat.-No AM06532SU-N against Hela (Lane 1), HEK293 (Lane 2) and Raji (Lane 3) cell lysate.
Immunohistochemical analysis of PAX8 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PAX8 expression in HeLa (A); HEK293T (B) whole cell lysates.
TA302560 (0.5µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample(30 ug whole cell lysate). A:293T. 10% SDS PAGE. TA308698 diluted at 1:1000
PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of PAX8 antibody (TA308698) The precipitated DNA was detected by PCR with primer set targeting to E2F1
PAX8 antibody detects PAX8 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. PAX8 antibody (TA308698) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8(TA308698) antibody at 1:200 dilution.
PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8
Predicted band size: 48 kDa . Positive control: MCF7 cell and mouse brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: MCF7 cell lysate Lane2: Mouse brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Predicted cell location: Nucleus. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using PAX8 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: 48 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Predicted cell location: Nucleus. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using PAX8 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
Immunohistochemistry staining of Paraffin Ovarian carcinoma (non-mucinous carcinoma) tissue by Pax-8 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear)
Host: Rabbit; Target Name: PAX8; Sample Tissue: Pancreas Tumor lysates; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-PAX8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: NCI-H226 cell lysate
Thyroid
WB Suggested Anti-PAX8 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
Host: Rabbit; Target Name: PAX8; Sample Tissue: THP-1 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308698 | PAX8 antibody |
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| Rabbit polyclonal antibody to PAX8CC (paired box 8) | |||||||
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Rabbit | IgG | Aff - Purified | Can, Hu, Ms, Rt, Xen | ChIP, ICC/IF, IP, P, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| AP16042PU-N | PAX8 (N-term) antibody |
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Goat | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
OriGene recommends this product for Western blotting. |
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| TA302560 | PAX8 antibody |
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| Goat Polyclonal Antibody against PAX8 (internal) | |||||||
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Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA321579 | PAX8 antibody |
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| Anti-PAX8 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321580 | PAX8 antibody |
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| Anti-PAX8 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA327724 | PAX8 antibody |
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| Mouse Monoclonal Pax-8 Antibody | |||||||
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Mouse | IgG | MRQ-50 | Purified | Hu | P |
1 ml /
€1,280.00
|
| OriGene Technologies, Inc. | |||||||
| AM06532SU-N | PAX8 antibody |
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 |
Mouse | IgG1 | 2D4 | Ascites | Hu | E, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
| AM10131SU-N | PAX8 antibody |
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Rabbit | IgG | ZR-1 | Supernatant | Hu | P |
1 ml /
€740.00
|
| OriGene Technologies GmbH | |||||||
| AP07520PU-N | PAX8 (N-term, all isoforms) antibody |
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 |
Goat | Aff - Purified | Can, Elephant, Hu, Mky, Ms, Platypus, Rb, Rt | E, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP17623PU-N | PAX8 (Center) antibody |
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Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| AP20584PU-N | PAX8 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300651 | PAX8 (transcript variant PAX8A) |
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| Recombinant protein of human paired box 8 (PAX8), transcript variant PAX8A | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP314188 | PAX8 (transcript variant PAX8D) |
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| Purified recombinant protein of Homo sapiens paired box 8 (PAX8), transcript variant PAX8D | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP315690 | PAX8 (transcript variant PAX8E) |
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| Recombinant protein of human paired box 8 (PAX8), transcript variant PAX8E | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY415574 | PAX8 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. | ||
| LY429402 | PAX8 overexpression lysate |
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0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207296L2V | Lenti ORF particles, Pax8 (GFP-tagged) - Mouse paired box gene 8 (Pax8), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,120.00
|
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| OriGene Technologies, Inc. | ||
| MR207296L1V | Lenti ORF particles, Pax8 (Myc-DDK-tagged) - Mouse paired box gene 8 (Pax8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,010.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR206306L2V | Lenti ORF particles, Pax8 (GFP-tagged ORF) - Rat paired box 8 (Pax8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||
| RR206306L1V | Lenti ORF particles, Pax8 (Myc-DDK-tagged ORF) - Rat paired box 8 (Pax8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,010.00
|
|
| OriGene Technologies, Inc. | ||