PBX1 antibody
Principal name
PBX1 antibody
Alternative names for PBX1 antibody
Homeobox protein PBX1, Homeobox protein PRL, Pre-B-cell leukemia transcription factor 1
Ncbi ID
9606, NP_002576, NP_002576.1, NP_001191892.1, NP_032809.1, BC002244, NM_183355, NM_002585
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of PBX1 antibody
- Kit Component:
- - KN312861G1, Pbx1 gRNA vector 1 in pCas-Guide vector
- KN312861G2, Pbx1 gRNA vector 2 in pCas-Guide vector
- KN312861D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Horman SR, Velu CS, Chaubey A, Bourdeau T, Zhu J, Paul WE, Gebelein B, Grimes HL. Gfi1 integrates progenitor versus granulocytic transcriptional programming.
Blood 2009 May 113 (22): 5466-75.
PMID: 19346496
Immunoperoxidase of monoclonal antibody to PBX1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to PBX1 on HeLa cell. [antibody concentration 10 ug/ml]
PBX1 monoclonal antibody (M01), clone 4A2 Western Blot analysis of PBX1 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged PBX1 is approximately 0.03ng/ml as a capture antibody.
PBX1 monoclonal antibody (M02), clone 3F7 Western Blot analysis of PBX1 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged PBX1 is approximately 0.3ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-PBX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; PBX1 antibody - N-terminal region (ARP30948_P050) in Human Jurkat cells using Western Blot
Western blot analysis: AP20176PU-N PBX1 antibody staining of K562 lysate at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-PBX1 mouse monoclonal antibody (TA502941) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PBX1(RC210944).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (RC210944, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PBX1.
Anti-PBX1 mouse monoclonal antibody (TA502971) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PBX1(RC210944).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (RC210944, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PBX1.
HEK293T cells transfected with either RC210944 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PBX1 antibody(TA502971), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PBX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503065)
Anti-PBX1 mouse monoclonal antibody (TA503065) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PBX1(RC210944).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (RC210944, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PBX1.
HEK293T cells transfected with either RC210944 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PBX1 antibody(TA503065), and then analyzed by flow cytometry.
Anti-PBX1 mouse monoclonal antibody (TA503230) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PBX1(RC210944).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (RC210944, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PBX1.
HEK293T cells transfected with either RC210944 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PBX1 antibody(TA503230), and then analyzed by flow cytometry.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PBX1 mouse monoclonal antibody (UM500077). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PBX1 mouse monoclonal antibody (UM500078). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
WB Suggested Anti-PBX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP20176PU-N | PBX1 antibody |
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Goat | Aff - Purified | Can, Hu, Ms, Rt | E, WB |
0.1 mg /
€380.00
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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