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PCDHGA8 antibody

Principal name

PCDHGA8 antibody

Alternative names for PCDHGA8 antibody

Protocadherin gamma-A8, PCDH-gamma-A8, KIAA0327

SwissProt ID

Q9Y5G5 (Human)

Gene ID

9708 (PCDHGA8)

Ncbi ID

9606, NP_054723.1, NM_032088, NP_054723

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PCDHGA8 antibody

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

General readings

Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
Wu, Q., et al. Cell 97(6):779-790(1999)

Western Blot analysis of PCDHGA8 expression in transfected 293T cell line by PCDHGA8 monoclonal antibody (M01), clone 1C11.Lane 1: PCDHGA8 transfected lysate(101.48 KDa).Lane 2: Non-transfected lysate.

H00009708-M01

Western blot analysis of PCDHGA8 over-expressed 293 cell line, cotransfected with PCDHGA8 Validated Chimera RNAi ( Cat # H00009708-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDHGA8 monoclonal antibody (M01), clone 1C11 (Cat # H00009708-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

H00009708-M01

Detection limit for recombinant GST tagged PCDHGA8 is approximately 0.3ng/ml as a capture antibody.

H00009708-M01

Western Blot analysis of PCDHGA8 expression in transfected 293T cell line (H00009708-T02) by PCDHGA8 MaxPab polyclonal antibody.Lane 1: PCDHGA8 transfected lysate(89.90 KDa).Lane 2: Non-transfected lysate.

H00009708-B01

PCDHGA8 Antibody (C-term) (Cat. #TA324456) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCDHGA8 antibody detected the PCDHGA8 protein (arrow).

TA324456

PCDHGA8 Antibody (C-term) (Cat. #TA324456)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHGA8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

TA324456

Confocal immunofluorescent analysis of PCDHGA8 Antibody (C-term)(Cat#TA324456) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

TA324456

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA324456	PCDHGA8 (C-term) antibody
	Rabbit polyclonal PCDHGA8 Antibody (C-term)
	Rabbit	Ig	Aff - Purified	Hu	ICC/IF, P, WB	0.4 ml / €415.00
						OriGene Technologies, Inc.
+2 additional images

Lysates

Catalog No.
LY410371	PCDHGA8 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC224312L2V	Lenti ORF particles, PCDHGA8 (mGFP-tagged) - Human protocadherin gamma subfamily A, 8 (PCDHGA8), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €2,050.00
		OriGene Technologies, Inc.

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