PCK2 antibody
Principal name
PCK2 antibody
Alternative names for PCK2 antibody
PCK-2, PEPCK2, Phosphoenolpyruvate carboxykinase, PEPCK-M, EC=4.1.1.32, Phosphoenolpyruvate carboxylase
Ncbi ID
5874, 9606, NP_001018083.1, NM_004563, NP_083270.1, BC010318, NM_028994, NM_001018073
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Enzyme Immunoassay (E)
Background of PCK2 antibody
- Kit Component:
- - KN200354G1, PCK2 gRNA vector 1 in pCas-Guide vector
- KN200354G2, PCK2 gRNA vector 2 in pCas-Guide vector
- KN200354D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Stark R, Pasquel F, Turcu A, Pongratz RL, Roden M, Cline GW, Shulman GI, Kibbey RG. Phosphoenolpyruvate cycling via mitochondrial phosphoenolpyruvate carboxykinase links anaplerosis and mitochondrial GTP with insulin secretion. J Biol Chem. 2009 Sep 25;284(39):26578-90. PMID: 19635791
2. Samuel VT, Beddow SA, Iwasaki T, Zhang XM, Chu X, Still CD, Gerhard GS, Shulman GI. Fasting hyperglycemia is not associated with increased expression of PEPCK or G6Pc in
patients with Type 2 Diabetes. Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):12121-6. PMID: 19587243.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot analysis of anti-PCK2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.
PCK2 MaxPab polyclonal antibody. Western Blot analysis of PCK2 expression in human liver.
Western Blot analysis of PCK2 expression in transfected 293T cell line (H00005106-T01) by PCK2 MaxPab polyclonal antibody.Lane 1: PCK2 transfected lysate(70.4 KDa).Lane 2: Non-transfected lysate.
PCK2 MaxPab polyclonal antibody. Western Blot analysis of PCK2 expression in human liver.
Western Blot analysis of PCK2 expression in transfected 293T cell line (H00005106-T01) by PCK2 MaxPab polyclonal antibody.Lane 1: PCK2 transfected lysate(70.4 KDa).Lane 2: Non-transfected lysate.
PCK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in human liver.
Western Blot analysis of PCK2 expression in transfected 293T cell line (H00005106-T02) by PCK2 MaxPab polyclonal antibody.Lane 1: PCK2 transfected lysate(70.70 KDa).Lane 2: Non-transfected lysate.
PCK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in HepG2.
PCK2 monoclonal antibody (M05), clone 2C9. Western Blot analysis of PCK2 expression in A-431 ( Cat # L015V1 ).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PCK2 polyclonal antibody ( Cat # PAB3311 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of PCK2 polyclonal antibody ( Cat # PAB3311 ) in A-375 cell lysate. PCK2 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
PCK2 polyclonal antibody ( Cat # PAB6875 ) ( 0.5 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
TA302563 (2 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302563 | PCK2 antibody |
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| Goat Polyclonal Antibody against PCK2 / PEPCK-M | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
Published recently! |
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| AP15051PU-N | PCK2 (N-term) antibody |
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY401446 | PCK2 overexpression lysate |
|
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0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218939L2V | Lenti ORF particles, Pck2 (GFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (Pck2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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200 µl /
€1,420.00
|
|
| OriGene Technologies, Inc. | ||
| MR209685L1V | Lenti ORF particles, Pck2 (Myc-DDK-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (cDNA clone MGC:11639 IMAGE:3596227), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR218939L1V | Lenti ORF particles, Pck2 (Myc-DDK-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (Pck2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,270.00
|
|
| OriGene Technologies, Inc. | ||