PCLO antibody
Principal name
PCLO antibody
Alternative names for PCLO antibody
Protein piccolo, Aczonin, ACZ, KIAA0559
Gene ID
27445 (PCLO)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F)
Background of PCLO antibody
- Kit Component:
- - KN212762G1, PCLO gRNA vector 1 in pCas-Guide vector
- KN212762G2, PCLO gRNA vector 2 in pCas-Guide vector
- KN212762D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual

Western Blot analysis of PCLO expression in transfected 293T cell line (H00027445-T01) by PCLO MaxPab polyclonal antibody.Lane 1: PCLO transfected lysate(39.16 KDa).Lane 2: Non-transfected lysate.

Western blot of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.

Western blot of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.

Western blot analysis of Piccolo in mouse and rat brain lysate, using a 1:1000 dilution of the antibody

Piccolo BTX. Neuromuscular junction whole musle samples from adult mouse, with 1:400 dilution of the antibody,secondary (Alexa 488 goat anti-Rabbit from invitrogen). It shows bright staining of the NMJ very selectively as well as some terminals, very brig
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA326449 | PCLO antibody |
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| Rabbit Polyclonal Anti-Piccolo Antibody | |||||||
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Rabbit | Purified | Hu, Ms | WB |
0.1 mg /
€410.00
|
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| OriGene Technologies, Inc. | |||||||
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| TA326497 | PCLO antibody |
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| Mouse Monoclonal Anti-Piccolo Antibody | |||||||
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Mouse | IgG2b | 6H9-B6 | Purified | Hu | WB |
0.1 mg /
€405.00
|
| OriGene Technologies, Inc. | |||||||
| AM26707PU-N | PCLO antibody |
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Mouse | IgG1 | PCLO-01 | Aff - Purified | Hu | F, WB |
0.1 mg /
€260.00
|
| OriGene Technologies GmbH | |||||||
| AM26707RP-N | PCLO antibody |
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Mouse | IgG1 | PCLO-01 | PE | Hu | F |
0.1 mg /
€290.00
|
| OriGene Technologies GmbH | |||||||



+1 additional image

